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1.
J Steroid Biochem Mol Biol ; 154: 267-73, 2015 Nov.
Article in English | MEDLINE | ID: mdl-26344639

ABSTRACT

The harderian gland (HG) is an orbital gland of the vast majority of land vertebrates. In the Syrian hamster these glands display a marked sexual dimorphism. Here we present data on a male specific clone named MHG30. The MHG30 cDNA (1470 bp) has significant sequence homologies with human #15µ10#Δ6-desaturase enzymes. The expression of MHG30 has been found in male HG and in the liver of both sexes, no other tissue showing the presence of MHG30 mRNA. Castration brings the MHG30 levels below detectable level in about 7 days. In in vitro cultures of male hamster HG cells, androgens (A) determine an enhancement of MHG30 expression in a time-dependent manner. Conversely, a continuous decrement has been observed in control cells and in cells treated with A plus flutamide (F) or with A and cycloheximide (Cy). Incubation of cells in cultures supplemented with desamethason (Dex) or thyroid hormone (T3) also increases MHG30 expression while 17ß-estradiol prevents the stimulatory effect exerted by A, Dex and T3. Findings strongly suggest that the MHG30 gene could be involved in supporting the sexual dimorphism and its expression is likely triggered by a series of hormonal interactions.


Subject(s)
Fatty Acid Desaturases/genetics , Gene Expression Regulation, Enzymologic/physiology , Harderian Gland/enzymology , Hormones/physiology , Animals , Base Sequence , Castration , Cricetinae , DNA, Complementary , Male , Mesocricetus , Molecular Sequence Data
2.
J Steroid Biochem Mol Biol ; 116(1-2): 56-60, 2009 Aug.
Article in English | MEDLINE | ID: mdl-19406238

ABSTRACT

Idiopathic scoliosis (IS) is a largely diffused disease in human population but its pathogenesis is still unknown. There is a relationship between scoliotic phenotype and the patient age, since in the early stage the pathology shows a ratio of 50% between male and female teenagers. During puberty the sex ratio is 8.4/1 (female/male), suggesting a sex-conditioned manifestation of the disease. Genetic inheritance of idiopathic scoliosis is still unclear although some authors claim for its X-linked dominant inheritance. There is large agreement in considering the IS as a sex-conditioned disease, in terms of steroid content and their receptor activity, although no evidence has been found yet. The blood content of 17beta-estradiol in teenagers with IS shows lower levels than teenagers of the same age without IS. Also testosterone and progesterone content are lower in IS girls with respect to the control girls. Furthermore, we extracted DNA from white blood cells of IS patients and their relatives until the third generation in order to examine estrogen receptor alpha polymorphisms, considering this tool a plausible molecular marker for IS prognosis. In this respect, we identified four polymorphisms in the exons encoding for the steroid binding domain and two other in the trans-activation domain. Our results show a clear relationship with clinical manifestation of IS.


Subject(s)
Estrogen Receptor alpha/genetics , Genetic Linkage/genetics , Polymorphism, Genetic , Scoliosis/genetics , Adolescent , Estradiol/blood , Estrogen Receptor alpha/metabolism , Exons , Female , Genetic Predisposition to Disease , Genotype , Humans , Phenotype , Progesterone/blood , Testosterone/blood , Young Adult
3.
Behav Brain Res ; 184(1): 91-100, 2007 Nov 22.
Article in English | MEDLINE | ID: mdl-17706799

ABSTRACT

The molecular hypothesis of learning and memory processes is based on changes in synaptic weights in neural networks. Aim of this study was to map neural traces of exposure to a spatial novelty were mapped by (i) the transcription factors (TFs) c-fos, c-jun and jun-B using Northern blot and immunocytochemistry (ICC), (ii) RNA synthesis by (3)H-uridine autoradiography and RNA level, (iii) NADPH-diaphorase (NADPH-d) expression by histochemistry. Thus, adult male albino rats were exposed to a Làt-maze and sacrificed at different times. Non-exposed rats served as controls. The latter showed a low constitutive expression of TF, RNA synthesis and NADPH-d across the brain. Northern blots showed a three-fold increase in TFs in exposed versus non-exposed rats in the cerebral cortex. ICC showed in exposed rats several TFs positive cells in the granular and pyramidal layers of the hippocampus and later in all layers of the somatosensory cortex, in the granular layer of the cerebellar cortex. The TF-positivity was stronger in rats exposed for the first time, and was time and NMDA-dependent. Autoradiography for RNA synthesis showed positive cells in the ependyma, hippocampus and cerebellum 6h after testing, and in the somatosensory cortex 24h later. In addition, exposure to novelty induced NADPH-d in the dorsal hippocampus, the caudate-putamen, all the layers of the somatosensory cortex. and the cerebellum. The positivity was absent immediately after exposure, appeared within 2h and disappeared 24h later. A strong neuronal discharge by the convulsant pentylenetetrazol, strongly induced TFs but not din not affect NADPH-d 2h later. Thus, data suggest that the processing of spatial and emotional components of experience activates neural networks across different organization levels of the CNS.


Subject(s)
Brain/metabolism , Exploratory Behavior/physiology , Gene Expression Regulation/physiology , NADPH Dehydrogenase/metabolism , RNA/metabolism , Spatial Behavior/physiology , Transcription Factors/metabolism , Analysis of Variance , Animals , Autoradiography/methods , Behavior, Animal , Emotions/physiology , Gene Expression Regulation/drug effects , Male , Maze Learning , Rats , Rats, Sprague-Dawley , Time Factors , Transcription Factors/genetics , Tritium/metabolism , Uridine/metabolism
4.
Comp Biochem Physiol B Biochem Mol Biol ; 132(1): 97-105, 2002 May.
Article in English | MEDLINE | ID: mdl-11997213

ABSTRACT

The androgen receptor (AR) must be considered a transcription factor belonging to the steroid-thyroid hormones receptor superfamily. Previous results gained from the Harderian gland, a tubulo-alveolar gland located in the orbital cavity of the golden hamster, indicate that Harderian gland cells express mRNAs encoding for androgen, glucocorticoid, thyroid hormone (T(3)), and estrogen receptors, respectively. Since in other systems, these receptors have been related to the expression of the androgen receptor, we have studied the regulation of AR expression in primary cultures of the male hamster Harderian gland. Our in vitro experiments show that androgen, and thyroid hormones increase the expression of AR. Retinoic acids also show a positive effect on AR expression, while exposure to glucocorticoid or estrogen blocks AR expression. Since these steroids differently modulate AR expression, our results must be considered in the context of multi-hormonal control of gene expression that could act through cross-talk between members of the steroid-thyroid hormones.


Subject(s)
Gene Expression Regulation , Harderian Gland/cytology , RNA, Messenger/metabolism , Receptors, Androgen/biosynthesis , Steroids/metabolism , Animals , Blotting, Northern , Cells, Cultured , Cricetinae , Cycloheximide/pharmacology , Dexamethasone/pharmacology , Estrogens/metabolism , Female , Glucocorticoids/metabolism , Male , Mesocricetus , Protein Synthesis Inhibitors/pharmacology , Receptors, Androgen/genetics , Thyroid Hormones/pharmacology , Time Factors , Tretinoin/metabolism , Tretinoin/pharmacology
5.
Gen Comp Endocrinol ; 119(2): 132-9, 2000 Aug.
Article in English | MEDLINE | ID: mdl-10936033

ABSTRACT

The hamster Harderian gland (HG), a compound tubuloalveolar gland located in the orbital cavity, displays sex dimorphism. The present study focuses on the sequence analysis of a cDNA clone named MHG07 and on the regulation of its expression by steroid hormones. MHG07 mRNA (5.0 kb) is expressed in male HG only. The MHG07 cDNA (1.74 kb) shows an ORF of 94 amino acids and has no significant homologies with other polypeptides/genes. Castration leads to the disappearance of MHG07 mRNA after 4 days, whereas treatment with testosterone impairs the effect of castration. No MHG07 mRNA has been found in either rat or murine HGs. Androgen (A) administration to female hamsters induces the appearance of MHG07 mRNA. In primary culture of male hamster HG, androgens increase the MHG07 expression and this effect is blocked by both flutamide and cycloheximide. Dose-response experiments show that, at low A concentration (10(-12) M), the MHG07 was higher than that of the control (2-fold). This effect reaches its zenith at 10(-8) M (10-fold). This picture is paralleled by androgen receptor mRNA expression. It is argued that the expression of MHG07 is under androgenic control.


Subject(s)
Androgens/pharmacology , Gene Expression Regulation/drug effects , Harderian Gland/metabolism , RNA, Messenger/metabolism , Sequence Analysis, DNA , Amino Acid Sequence , Androgens/physiology , Animals , Base Sequence , Blotting, Northern , Cricetinae , Culture Techniques , DNA, Complementary/chemistry , Female , Harderian Gland/chemistry , Male , Mesocricetus , Molecular Sequence Data , Orchiectomy , RNA, Messenger/analysis , Receptors, Androgen/genetics , Sex Characteristics , Testosterone/pharmacology
6.
J Steroid Biochem Mol Biol ; 72(3-4): 133-41, 2000 Mar.
Article in English | MEDLINE | ID: mdl-10775804

ABSTRACT

The network of hormonal and non-hormonal signals required for testicular activity during the reproductive cycle of the seasonal breeding lizard, Podarcis sicula, are not yet well understood. Androgens are significantly involved in meiosis and spermiogenesis, and such an effect is mediated through their receptor (AR). Estrogens also affect the testicular activity down-regulating the expression of AR mRNA. Since over the last few years, extensive works have reported, in mammals, a clear influence of tri-iodothyronine (T(3)), the biologically active thyroid hormone, on Sertoli cell activities, we carried out a study to shead light on the effect/s exerted by T(3) in lizard testis. A thyroid hormone receptor mRNA (TR mRNA) has been found in the testis indicating that T(3) might be involved in the regulation of gonadal activity. In in vivo experiments, injection of T(3) to male lizards, captured during the recrudescence period (March) and maintained under experimental photothermal conditions (24 degrees C and 15 h daylight), increased the expression of AR mRNA. The in vitro results confirmed the stimulatory effect of T(3) on AR mRNA levels. Thus, in testosterone (T) exposed cells, the highest values of AR mRNA were observed in T(3)-primed animals, indicating that T and T(3) increase AR gene transcription independently. The present data suggest that, in lizards, the combined action of androgens, estrogen and T(3) might regulate testicular activity, modulating AR mRNA levels.


Subject(s)
Lizards/genetics , Lizards/metabolism , RNA, Messenger/genetics , RNA, Messenger/metabolism , Receptors, Androgen/genetics , Testis/drug effects , Testis/metabolism , Triiodothyronine/pharmacology , Actins/genetics , Animals , Cells, Cultured , Gene Expression/drug effects , Male , Receptors, Thyroid Hormone/genetics
7.
Gen Comp Endocrinol ; 110(3): 227-36, 1998 Jun.
Article in English | MEDLINE | ID: mdl-9593644

ABSTRACT

Steroid hormones regulate many developmental and physiological processes via specific receptors whose number can be up- or downregulated. The regulation of estrogen (ER) and androgen (AR) receptor mRNAs in primary cultures of lizard testis is described. The high degree of homology between the probes used and the receptor mRNAs in lizard testis was consistent with the high-stringency hybridisation conditions and the molecular size of ER mRNAs (7.4 and 4.5 kb) and AR mRNA (9.5 kb). Primary cultures of testis cells revealed a time- and drug-dependent relationship between ER and AR mRNAs. 17beta-oestradiol (E) autoregulated ER mRNA and downregulated AR mRNA. The antiestrogen ICI 164,384 reversed the latter effect. Cycloheximide (Cy), to inhibit protein synthesis, in combination with E, impaired the AR mRNA expression. Testosterone (T) autoregulated the expression of its own receptor mRNA whereas this effect was reversed by both flutamide (F) and Cy. Dose-response experiments showed that low concentrations of steroids (E or T 10(-12) M) increased ER or AR mRNA levels, respectively. These results suggest that both estrogen and androgen may autoregulate the expression of their own receptor mRNAs. Since in lizard testis androgens are significantly involved in meiosis and spermiogenesis and E dramatically impairs the AR mRNA expression, the latter effect may be key in regulating certain phases of reproduction.


Subject(s)
Down-Regulation/genetics , Estradiol/pharmacology , Homeostasis/genetics , Lizards/physiology , RNA, Messenger/drug effects , Receptors, Androgen/drug effects , Receptors, Estrogen/drug effects , Testis/metabolism , Animals , Blotting, Northern , Cell Separation , Cells, Cultured , Dose-Response Relationship, Drug , Down-Regulation/drug effects , Gene Expression Regulation/drug effects , Homeostasis/drug effects , Lizards/genetics , Lizards/metabolism , Male , RNA, Messenger/biosynthesis , Receptors, Androgen/genetics , Receptors, Estrogen/genetics , Testis/cytology , Testis/drug effects , Time Factors
8.
J Steroid Biochem Mol Biol ; 62(5-6): 455-60, 1997 Aug.
Article in English | MEDLINE | ID: mdl-9449249

ABSTRACT

Xenopus laevis shows a sexual dimorphism of the electrophoretic pattern of Harderian gland (HG) proteins. The male pattern displays three protein fractions whose molecular sizes are approx. 205, 180 and 78 kDa, respectively, and which are absent in the female pattern. Conversely, the female pattern displays two protein fractions of approx. 190 and 76 kDa, respectively. This sexual dimorphism led us to hypothesize a sex steroid control of the HG. Administration of 17beta-oestradiol to male Xenopus converts the male protein pattern into the female one, while the administration of testosterone to the female has no effect. In this respect neither Northern analysis nor the RNase-protection assay performed using a 213 bp encoding for the androgen-binding domain reveals the presence of an androgen receptor mRNA in Xenopus HG. Conversely, Northern analysis has shown an oestrogen receptor mRNA whose size is approx. 6.5 kb and the RNase-protection assay performed by using a 197 bp encoding for the oestrogen-binding domain has also displayed the presence of an oestrogen receptor mRNA in the female HG but not in the male one. In addition, the oestrogen administration to male Xenopus induces the appearance of an oestrogen receptor mRNA. Androgen administration to female toad is ineffective. Taken together, all these findings suggest that in Xenopus laevis oestrogens are involved into the HG physiology. The appearance of an oestrogen receptor mRNA in the oestradiol treated males supports the hypothesis of the occurrence of autoinduction of oestrogen receptor mRNA expression in the HG.


Subject(s)
Estradiol/pharmacology , Harderian Gland/drug effects , Harderian Gland/metabolism , Sex Characteristics , Xenopus laevis/metabolism , Animals , Female , Harderian Gland/growth & development , Male , Molecular Weight , Proteins/chemistry , Proteins/isolation & purification , Proteins/metabolism , RNA, Messenger/genetics , RNA, Messenger/metabolism , Receptors, Androgen/genetics , Receptors, Estrogen/genetics , Testosterone/pharmacology , Xenopus laevis/genetics , Xenopus laevis/growth & development
9.
Mol Cell Endocrinol ; 124(1-2): 87-96, 1996 Nov 29.
Article in English | MEDLINE | ID: mdl-9027328

ABSTRACT

The regulation of the FHG22 gene by sex steroids has been studied in Syrian hamster Harderian gland, an organ with sexual dimorphism in which the FHG22 mRNA is female-specific. Testosterone treatment of females caused irregular inhibitory effects on the FHG22 mRNA levels, whereas male castration originated transitory increases during less than 2 weeks. Treatment of 15 day-castrated males for 1 or 2 days with beta-estradiol-3-benzoate caused a marked stimulation in the FHG22 mRNA levels. The results found in vivo may be explained considering those found in female Harderian gland serum-free primary cell cultures. In the absence of hormones, the FHG22 mRNA levels decreased along the time and neither progesterone, testosterone, or 5 alpha-dihydrotestosterone affected the expression. However, estradiol stimulated the FHG22 mRNA expression in a time and dose-dependent manner: increasing effects were detected between 8-96 h of treatment and the EC50 was about 10(-9) M. The estradiol effect was reverted by the antiestrogen ICI 164,384 or by cycloheximide. We conclude that estradiol stimulates FHG22 mRNA expression in Harderian gland, although other agents may also control the expression in vivo.


Subject(s)
Dihydrotestosterone/pharmacology , Estradiol/pharmacology , Gene Expression Regulation/drug effects , Harderian Gland/physiology , Animals , Cells, Cultured , Cricetinae , Cycloheximide/pharmacology , Dose-Response Relationship, Drug , Estradiol/analogs & derivatives , Estrogen Antagonists/pharmacology , Female , Gene Expression Regulation/physiology , Male , Mesocricetus , Orchiectomy , Polyunsaturated Alkamides , Protein Synthesis Inhibitors/pharmacology , RNA, Messenger/analysis , Sex Factors
10.
Gen Comp Endocrinol ; 102(3): 386-93, 1996 Jun.
Article in English | MEDLINE | ID: mdl-8804569

ABSTRACT

The Harderian gland (hg) is a gland which occupies a large portion of the orbital cavity. In many species, a sexual dimorphism occurs, which suggests a gonadal steroid control of the hg. The present study examines, in primary cultures of hamster hg cells, the regulation of the androgen receptor mRNA (AR mRNA) expression. In dose-response experiments measuring the expression of AR mRNA, testosterone (T) (10(-12) M) induced a 1-fold increase of AR mRNA compared with unexposed cells, and this effect reached its zenith (6.2-fold) when cells were exposed to 10(-8) M T. In other experiments, cells were exposed or not to different drugs [T, T + flutamide (F), F, T + cycloheximide (Cy), Cy] for different times (up to 96 hr). These experiments showed a time-dependent increase of AR mRNA in the cells exposed to T, while in the cells exposed to F, T + F, T + Cy, Cy, and control (unexposed), a time-dependent decrease of AR mRNA was observed. The size of the hamster AR mRNA in these in vitro experiments was similar to that observed in other mammals (9.5 kb). It is concluded that primary cultures of hamster hg cells are a valuable model for studying hg cell activity and that in this system T autoinduces its own receptor.


Subject(s)
Gene Expression Regulation/drug effects , Harderian Gland/metabolism , RNA, Messenger/biosynthesis , Receptors, Androgen/genetics , Actins/genetics , Actins/standards , Androgen Antagonists/pharmacology , Animals , Autoradiography , Blotting, Northern , Cells, Cultured , Cricetinae , Cycloheximide/pharmacology , DNA, Complementary , Dose-Response Relationship, Drug , Flutamide/pharmacology , Harderian Gland/cytology , Harderian Gland/drug effects , Male , Mesocricetus , Phosphorus Radioisotopes , Protein Synthesis Inhibitors/pharmacology , Rats , Rats, Sprague-Dawley , Receptors, Androgen/drug effects , Reference Standards , Testosterone/pharmacology , Time Factors
11.
Microsc Res Tech ; 34(2): 97-103, 1996 Jun 01.
Article in English | MEDLINE | ID: mdl-8722702

ABSTRACT

In Rana esculenta the presence of an androgen receptor in both the male and female Harderian gland (HG) has been demonstrated. Hybridization analysis has evidenced a high degree of homology between the rat androgen receptor cDNA and the frog androgen receptor mRNA (fARmRNA). Correspondingly the molecular size of fARmRNA is similar to those described in mammals (9.4 kb). In in vivo experiments testosterone (T) increases the levels of fARmRNA. The use of the antiandrogen alone or in combination with T prevents the increase of fARmRNA. In the control animals a loss of fARmRNA has been observed. In primary cultures of HG cells, the steady-state levels of fARmRNA increase in the cells exposed to T. These results suggest that T exerts an autoinduction on its own receptor, increasing the levels of fARmRNA. In Xenopus laevis the HG shows a sexual dimorphism of the protein pattern. The female shows two major proteins (210 and 180 kDa). Administration of estradiol to the male shifts the protein pattern into the female one. In this respect an estrogen receptor mRNA (ERmRNA) has been found in the female gland and can be induced in the male one. No ARmRNA has been detected in either sexes. A similar sex dimorphism has been found in Gallus domesticus. The female pattern is characterised by a protein fraction of about 210 kDa, the male one by a protein fraction of about 180 kDa. In 4-day-old chicks no sex differences have been found. An ERmRNA is expressed in the female, while no ARmRNA has been detected in both sexes. Neither AR nor ER mRNAs have been detected in the chick HG. Among mammals the HG or the hamster (Mesocricetus auratus) shows an androgen-dependent sex dimorphism. In in vitro experiments T 10(-12) M induces a onefold increase of ARm-RNA with respect to unexposed cells. This effect reaches its maximum (4.4-fold) when cells are exposed to T 10(-8) M. The size of the hamster ARmRNA is similar to that observed in other mammals (9.5 kb). The above results suggest that in the HG the phenomenon of autoinduction occurs and that there is a relationship between the androgen or estrogen dependence of the HG and the digamety of the species.


Subject(s)
Harderian Gland/chemistry , RNA, Messenger/metabolism , Receptors, Androgen/genetics , Receptors, Estrogen/genetics , Animals , Chickens , Chromosome Mapping , Cricetinae , Drosophila , Female , Humans , Male , Mice , Phylogeny , Rana esculenta , Rats , Transcription, Genetic , Xenopus laevis
12.
Int Rev Cytol ; 168: 1-80, 1996.
Article in English | MEDLINE | ID: mdl-8843648

ABSTRACT

The harderian gland is an orbital gland of the majority of land vertebrates. It is the only orbital gland in anuran amphibians since the lacrimal gland develops later during phylogenesis in some reptilian species. Perhaps because it is not found in man, little interest was paid to this gland until about four decades ago. In recent years, however, the scientific community has shown new interest in analyzing the ontogenetic and morphofunctional aspects of the harderian gland, particularly in rodents, which are the preferred experimental model for physiologists and pathologists. One of the main characteristics of the gland is the extreme variety not only in its morphology, but also in its biochemical properties. This most likely reflects the versatility of functions related to different adaptations of the species considered. The complexity of the harderian gland is further shown in its control by many exogenous and endogenous factors, which vary from species to species. The information gained so far points to the following functions for the gland: (1) lubrication of the eye and nictitating membrane, (2) a site of immune response, particularly in birds, (3) a source of pheromones, (4) a source of saliva in some chelonians, (5) osmoregulation in some reptiles, (6) photoreception in rodents, (7) thermoregulation in some rodents, and (8) a source of growth factors.


Subject(s)
Harderian Gland , Animals , Harderian Gland/anatomy & histology , Harderian Gland/metabolism , Harderian Gland/pathology , Humans , Molecular Biology , Receptors, Cell Surface
13.
J Steroid Biochem Mol Biol ; 51(5-6): 259-65, 1994 Dec.
Article in English | MEDLINE | ID: mdl-7826887

ABSTRACT

Alpha 32P-labelled cDNA probe from plasmid containing rat androgen receptor (rAR) has been tested in hybridization experiments using RNAs from the Harderian gland and thumb pad of the edible frog, Rana esculenta. Northern blot analysis has shown a high degree of homology between the rAR cDNA and the frog androgen receptor mRNA (fAR mRNA); this has been supported by both the hybridization conditions (high stringency) and the molecular size of fAR mRNA which is quite similar to those described in mammals (9.4 kb). The role of androgens has been further investigated with respect to the kinetics of expression of fAR mRNA in in vivo experiments. In both the Harderian gland and thumb pad, testosterone has increased the levels of fAR mRNA as compared with the untreated groups. The use of cyproterone acetate (CPA) in combination with testosterone has resulted in a loss of the increase in fAR mRNA as compared to testosterone-treated groups, while CPA alone has resembled the control group. In primary cultures of frog Harderian gland and thumb pad cells, the steady-state levels of fAR mRNA have been increased in the cells exposed to testosterone as compared to those not exposed. These findings confirm that, in these androgen target tissues, testosterone exerts an up-regulation on its own receptors, increasing the accumulation of fAR mRNA in the same way as oestrogens up-regulate the expression of their own receptors in Xenopus liver and oviduct cells.


Subject(s)
Harderian Gland/metabolism , Rana esculenta/metabolism , Receptors, Androgen/genetics , Testosterone/physiology , Animals , Blotting, Northern , Cells, Cultured , Cyproterone Acetate/pharmacology , Male , Organ Specificity , RNA, Messenger/biosynthesis , Rana esculenta/genetics , Rats , Rats, Sprague-Dawley , Reproduction/physiology , Up-Regulation
14.
Cell Tissue Res ; 273(2): 201-8, 1993 Aug.
Article in English | MEDLINE | ID: mdl-8364965

ABSTRACT

The effects of gonadectomy and testosterone treatment on the fine structure of the Harderian gland in male and female green frogs were investigated in different periods of the year. Gonadectomy, carried out when the glands are in the lowest secretory phase (September), causes degenerative changes consisting of a reduction of the rough endoplasmic reticulum, the appearance of autolysosomes, and an increase of nuclear heterochromatin. These effects can be prevented by testosterone treatment. No castration effects are found during the recovery (November) and enhancement (April-May) phases of secretory activity. The results suggest that the frog Harderian gland's sensitivity to testosterone changes during the annual cycle. The androgen dependence of the Harderian gland is correlated with the presence of androgen receptors in both male and female frogs.


Subject(s)
Harderian Gland/drug effects , Rana esculenta/anatomy & histology , Testosterone/pharmacology , Animals , Female , Harderian Gland/ultrastructure , Male , Microscopy, Electron , Orchiectomy , Ovariectomy , Ovary/physiology , Seasons , Testis/physiology
15.
Comp Biochem Physiol B ; 102(3): 601-3, 1992 Jul.
Article in English | MEDLINE | ID: mdl-1499297

ABSTRACT

1. Sodium dodecyl sulphate 7-12% gradient polyacrylamide gel electrophoresis of male and female hamster Harderian gland whole homogenate shows a clear-cut sexual dimorphism, which consists of the presence of two male-specific glycoproteins (168 and 116 kDa) and two specific female proteins (210 and 190 kDa). 2. In the male, castration causes a significant decrease in the concentration of the two glycoprotein fractions. 3. Replacement therapy with testosterone propionate (T) restores the intact male pattern.


Subject(s)
Glycoproteins/metabolism , Harderian Gland/metabolism , Animals , Cricetinae , Electrophoresis, Polyacrylamide Gel , Female , Glycoproteins/isolation & purification , Harderian Gland/drug effects , Male , Mesocricetus , Orchiectomy , Sex Characteristics , Testis/physiology , Testosterone/pharmacology
16.
Mol Cell Endocrinol ; 84(3): R51-6, 1992 Apr.
Article in English | MEDLINE | ID: mdl-1375172

ABSTRACT

The role of androgens in the cyclic secretory activity of the Rana esculenta Harderian gland (HG) was studied. Total RNA showed a dramatic increase in October and May when the nuclear androgen receptors peak. During the resumption of the secretory activity a gradual increase of poly(A)(+)-RNA was detected; during the enhancement phase (May) a peak of the poly(A)(+)-RNA fraction was found. In in vitro experiments testosterone increased the incorporation of [3H]uridine into the poly(A)(+)-RNA fraction and also that of [35S]methionine into a newly synthesized protein fraction (100 kDa). The latter effect is prevented by the exposure of the cells to the antiandrogen, cyproterone acetate (CPA). These findings reveal that, besides hamsters, the HG is a target for androgens in the frog.


Subject(s)
Harderian Gland/metabolism , Poly A/biosynthesis , Protein Biosynthesis , RNA/biosynthesis , Testosterone/pharmacology , Animals , Cyproterone/analogs & derivatives , Cyproterone/pharmacology , Cyproterone Acetate , Male , Methionine/metabolism , RNA, Messenger , Rana esculenta , Uridine/metabolism
17.
Eur J Histochem ; 36(1): 81-90, 1992.
Article in English | MEDLINE | ID: mdl-1374668

ABSTRACT

When Mallory's trichrome stain is used, acinar nuclei of the Harderian gland of Rana esculenta display different affinities for the dye. Some of the orangiophilic nuclei show affinity for aniline blue (blue nuclei). In the Harderian gland of Rana esculenta their number and the intensity of staining with aniline blue may vary during the year. The affinity for aniline blue disappears following digestion of paraffin sections with RNAase, but not with DNAase or trypsin. Furthermore, in vitro incubation with [5, 6-3H]-Uridine shows a selective incorporation by the majority of blue nuclei. Therefore, the affinity for aniline blue is likely due to increased RNA synthesis. The increment of nuclear RNA shown by these methods is supported by the quantitative determination of total RNAs during the resumption (October) and enhancement (May) of secretory activity, when the percentage of blue nuclei of the acinar cells is at its highest levels of the year. The affinity of RNA-rich nuclei for aniline blue, while others are strictly orangiophil, is discussed on the basis of molecular structure of the dyes used in the staining mixture. Mallory's trichrome stain appears to be an useful tool for detecting changes in cell nuclear status.


Subject(s)
Harderian Gland/metabolism , RNA/biosynthesis , Staining and Labeling , Animals , Autoradiography , Cell Differentiation , Female , Harderian Gland/ultrastructure , Male , RNA/analysis , Rana esculenta
18.
J Endocrinol ; 129(2): 227-32, 1991 May.
Article in English | MEDLINE | ID: mdl-2040857

ABSTRACT

An androgen receptor has been identified in the cytosolic and nuclear extracts of the Harderian gland of the frog, Rana esculenta. A single class of high-affinity binding sites was found: Kd = 1.9 +/- 1.3 (S.D.) nmol/l (n = 26) for the cytosolic extract and Kd = 0.9 +/- 0.8 nmol/l (n = 15) for the nuclear extract. The presence of binding activity in both nuclear and cytosolic extracts and the low rate of ligand-receptor dissociation are characteristics that distinguish this receptor from a steroid-binding protein. The Kd did not show any sex difference and did not exhibit any secretory activity-related change. Binding in both cytosolic and nuclear extracts was specific for androgens (testosterone = 5 alpha-dihydrotestosterone); oestradiol-17 beta showed a 30% cross-reaction; moreover, specific binding of [3H]oestradiol-17 beta was not detectable. The binding capacity of the Harderian gland increased progressively in both fractions from October to December, reaching a peak in May, and decreased suddenly during July to August. The lack of any morphological sex-related difference in the Harderian gland of the green frog might be accounted for by the high amount of circulating androgens as well as a similar concentration of androgen receptor in both sexes.


Subject(s)
Harderian Gland/metabolism , Rana esculenta/metabolism , Receptors, Androgen/analysis , Animals , Binding, Competitive , Cell Nucleus/metabolism , Cytosol/metabolism , Female , Kinetics , Male , Seasons , Testosterone/metabolism
19.
Mol Cell Endocrinol ; 71(3): R25-31, 1990 Jul 09.
Article in English | MEDLINE | ID: mdl-2210028

ABSTRACT

The number of nuclear and cytosolic estrogen receptors (ER) per cell and the steady-state levels of the mRNA encoding a tissue-specific, estrogen-inducible protein (FOSP-1) were measured as a function of time following the addition of estradiol-17 beta (E2) to primary cultures of Xenopus oviduct cells. After a lag period of about 12 h, 10(-9) to 10(-7) M E2 caused a 10 to 15-fold increase in FOSP-1 mRNA by 60 h, whereas it was only 2-fold with 10(-7) M progesterone. Under the same conditions, E2 doubled its own total receptor content within the first 12 h, reaching a 4-fold increase in nuclear ER by 48 h. Cycloheximide treatment in the presence of 10(-7) M estradiol reduced the functional ER content by 75.90%. Treatment with the anti-estrogen ICI 164,384 of oviduct cells in which FOSP-1 mRNA was pre-induced to high levels with the hormones caused a drastic reduction in nuclear ER and a total loss of FOSP-1 mRNA in 72 h. The close correlation between the kinetics of autoinduction of ER and the induction of FOSP-1 mRNA, as was shown earlier for vitellogenin mRNA in hepatocytes (Perlman et al. (1984) Mol. Cell. Endocrinol. 38, 151-161), strongly suggests that Xenopus egg protein gene activation by estrogen requires the up-regulation of its own receptor by the hormone.


Subject(s)
Estradiol/pharmacology , Oviducts/metabolism , Protein Biosynthesis , Receptors, Estrogen/biosynthesis , Up-Regulation/drug effects , Animals , Cell Nucleus , Cells, Cultured , Cytosol , Female , Gene Expression Regulation/drug effects , Oviducts/drug effects , Progesterone/pharmacology , Proteins/genetics , RNA, Messenger/biosynthesis , Receptors, Estrogen/genetics , Transcriptional Activation , Xenopus Proteins , Xenopus laevis
20.
J Reprod Fertil ; 83(2): 825-8, 1988 Jul.
Article in English | MEDLINE | ID: mdl-3411572

ABSTRACT

Ile-de-France ewes had high plasma progesterone concentrations during early summer-late winter. Gentile-di-Puglia ewes had high progesterone values during the winter-spring-summer period but during autumn progesterone values were very low and oestrous behaviour was not displayed. The comparison with Ile-de-France ewes indicates that a phase shift occurs in the annual ovarian activity in ewes of the Gentile-di-Puglia breed.


Subject(s)
Progesterone/blood , Seasons , Sheep/blood , Animals , Female , Light , Ovary/physiology
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