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1.
J Biosci Bioeng ; 133(1): 8-16, 2022 Jan.
Article in English | MEDLINE | ID: mdl-34629297

ABSTRACT

Bacterial laccases have proven to be a potential biocatalyst for various industrial applications due to their remarkable catalytic and stability properties. In this study, a novel thermostable laccase was produced from the bacterium Bacillus licheniformis VNQ by submerged fermentation. The specific activity of crude and purified laccase was found to be 13.17 U mg-1 and 83.47 U mg-1, respectively. The enzyme possessed a molecular mass of ∼48 kDa when characterized by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The optimum temperature and pH for enzyme activity was determined to be 55°C and 5.0, respectively. The enzyme was considered to be thermo-tolerant as it possessed a half-life of 4 h at 70°C. The enzyme was utilized for the oxidative biotransformation of in situ synthesized p-quinones to biologically active compounds, 1,4-naphthoquinone and its derivative. The obtained products were characterized using nuclear magnetic resonance (NMR) spectroscopy and gas chromatography-mass spectrometry (GC-MS) analysis. A high yield of naphthoquinones (74.93 ± 1.2%) with 1,4-naphthoquinone (60.61 ± 1.0%), and its derivative 2-hydroxy-1,4-naphthoquinone (14.32 ± 0.2%) was obtained at the optimized reaction conditions.


Subject(s)
Bacillus licheniformis , Naphthoquinones , Bacillus licheniformis/metabolism , Electrophoresis, Polyacrylamide Gel , Enzyme Stability , Hydrogen-Ion Concentration , Laccase/metabolism , Substrate Specificity , Temperature
2.
Data Brief ; 14: 524-530, 2017 Oct.
Article in English | MEDLINE | ID: mdl-28856183

ABSTRACT

The dataset depicted in this article related to our earlier article entitled "Phytofabrication and encapsulated of silver nanoparticles from Gloriosa Superba" (Saradha Devi et al., 2017) [1], which reports the characteristic features (UV Visible spectra, FTIR, SEM, TEM, DLS, Zeta potential and XRD analysis) of the Gloriosa superba biosynthesised silver nanoparticles (AgNPs). In this context, the present dataset was provided to identify the antioxidant, antitumor and apoptotic (in DLA cells) properties with the synthesized AgNPs. The result enlightens the AgNPs exhibits antitumor, apoptotic activity in DLA cells and antioxidant properties. The results of the in vivo experiments increased life span of liver cells in DLA induced tumour mice and not showed any histopathological variations between control and DLA induced mice animals. The HPTLC examination of the Gloriosa superba (L.) seed extract infers the presence of colchicines derivatives as a major alkaloid sources.

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