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1.
Int J Biol Macromol ; 115: 106-113, 2018 Aug.
Article in English | MEDLINE | ID: mdl-29649535

ABSTRACT

A fucomannogalactan from Rhizoctonia solani biomass was obtained after hot aqueous extraction and purified by freeze-thaw cycles and gel filtration chromatography on Sepharose CL-6B. The polysaccharide was homogeneous after HPSEC/RID analysis (Mw/Mn~1.1), displaying an average molecular weight of 15.4×103Da. Its chemical structure was determined by methylation analysis (GC/MS) and spectroscopy (FTIR, 1D and 2D NMR). The polysaccharide had a branched α-1,6-linked Galp backbone with 66% linear residues, a number of which were at O-3 methylated. Side chains (34%) were always linked at O-2 positions of the main chain and consisted of single, non-reducing ends of α-d-Manp (6%) and α-l-Fucp (28%). Analysis of its biological activity showed that the highly purified fucomannogalactan from R. solani inhibited the proliferation of colon cancer cells in vitro, but that it did not have the same activity against lung cancer cells.


Subject(s)
Antineoplastic Agents/chemistry , Antineoplastic Agents/pharmacology , Galactans/chemistry , Polysaccharides/chemistry , Polysaccharides/pharmacology , Rhizoctonia/chemistry , Antineoplastic Agents/isolation & purification , Cell Line, Tumor , Cell Proliferation/drug effects , Colonic Neoplasms/pathology , Humans , Molecular Weight , Polysaccharides/isolation & purification , Water/chemistry
2.
Carbohydr Polym ; 98(2): 1353-9, 2013 Nov 06.
Article in English | MEDLINE | ID: mdl-24053813

ABSTRACT

A water-soluble polysaccharide was extracted with alkali from the cell wall of Verticillium lecanii (also called Lecanicillium lecanii). After freezing and thawing, the water-soluble fraction was purified by gel filtration chromatography on Sepharose CL-6B and eluted as one peak by HPSEC/RID. Monosaccharide analysis showed galactose and glucose (1.1:1), with traces of mannose (<1%). The structural characteristics were determined by spectroscopic analysis, FT-IR and 1D and 2D (1)H and (13)C NMR, and methylation results. On the basis of the data obtained, the following structure of the polysaccharide (E3SIV fraction) was established: [Formula: see text] where n≈22 and m≈22.


Subject(s)
Cell Wall/chemistry , Galactans/chemistry , Verticillium/chemistry , Carbohydrate Sequence , Chromatography, Gel , Galactans/isolation & purification , Galactose/chemistry , Glucose/chemistry , Magnetic Resonance Spectroscopy , Mannose/chemistry , Molecular Sequence Data , Sepharose/analogs & derivatives , Solubility , Spectroscopy, Fourier Transform Infrared , Water
3.
Molecules ; 16(9): 7488-501, 2011 Sep 02.
Article in English | MEDLINE | ID: mdl-21892127

ABSTRACT

Differential scanning calorimetry (DSC), thermogravimetry (TG) and Fourier-transform infra-red spectroscopy (FT-IR) analyses were performed to investigate changes in the physico-chemical properties of botryosphaerans, a family of exopolysaccharides (EPS) produced by the fungus Botryosphaeria rhodina MAMB-05 grown on glucose (EPS(GLC)), sucrose (EPS(SUC)) and fructose (EPS(FRU)). A slight endothermic transition and small mass loss attributable to the removal of water of hydration were observed in the DSC and TG analyses, respectively, for the three EPS samples. The FT-IR spectra confirmed no structural changes occurred during thermal treatment. Viscometry was utilized to obtain information on the rheological behaviour of the EPS in aqueous solutions. The Power Law and Cross Equations determined the natural pseudoplastic characteristics of the EPS. Comparatively, results obtained for EPS produced when B. rhodina MAMB-05 was grown on each of the three carbohydrate sources demonstrated similar apparent viscosity values for EPS(GLC) and EPS(SUC), while EPS(FRU) displayed the lowest apparent viscosity of the three botryosphaerans, suggesting a higher degree of ramification and lower Mw. EPS(GLC) and EPS(SUC) possessed similar degrees of ramification. The slight differences found in their viscosities can be explained by the differences in the type of branching among the three botryosphaerans, thus varying the strength of intermolecular interactions and consequently, consistency and viscosity. The physico-chemical studies of botryosphaerans represent the originality of this work, and the knowledge of these properties is an important criterion for potential applications.


Subject(s)
Glucans/chemistry , Saccharomycetales/metabolism , Calorimetry, Differential Scanning , Culture Media, Conditioned , Fructose/metabolism , Glucans/isolation & purification , Glucans/metabolism , Glucose/metabolism , Molecular Weight , Rheology , Spectroscopy, Fourier Transform Infrared , Sucrose/metabolism , Thermogravimetry , Viscosity
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