Nitrogen removal in a shallow maturation pond with sludge accumulated during 10 years of operation in Brazil.

Accumulated sludge in polishing (maturation) ponds reduces the hydraulic retention time (smaller useful volume), and this could potentially lead to a decrease in performance. However, settled biomass, present in the sediments, can contribute to nitrogen removal by different mechanisms such as nitrification and denitrification. This study investigated the influence of the bottom sludge present in a shallow maturation pond treating the effluent from an anaerobic reactor on the nitrification and denitrification processes. Nitrification and denitrification rates were determined in sediment cores by applying ammonia pulses. Environmental conditions in the medium were measured and bacteria detected and quantified by real-time polymerase chain reaction (real-time PCR). The pond showed daily cycles of mixing and stratification and most of the bacteria involved in nitrogen removal decreased in concentration from the upper to the lower part of the sludge layer. The results indicate that denitrifiers, nitrifiers and anammox bacteria coexisted in the sludge, and thus different metabolic pathways were involved in ammonium removal in the system. Therefore, the sediment contributed to nitrogen removal, even with a decrease in the hydraulic retention time in the pond due to the volume occupied by the sludge.

Field application of a planted fixed bed reactor (PFR) for support media and rhizosphere investigation using undisturbed samples from full-scale constructed wetlands.

This study presents a novel method for investigations on undisturbed samples from full-scale horizontal subsurface-flow constructed wetlands (HSSFCW). The planted fixed bed reactor (PFR), developed at the Helmholtz Center for Environmental Research (UFZ), is a universal test unit for planted soil filters that reproduces the operational conditions of a constructed wetland (CW) system in laboratory scale. The present research proposes modifications on the PFR original configuration in order to allow its operation in field conditions. A mobile device to obtain undisturbed samples from real-scale HSSFCW was also developed. The experimental setting is presented with two possible operational configurations. The first allows the removal and replacement of undisturbed samples in the CW bed for laboratory investigations, guaranteeing sample integrity with a mobile device. The second allows the continuous operation of the PFR and undisturbed samples as a fraction of the support media, reproducing the same environmental conditions outside the real-scale system. Investigations on the hydrodynamics of the adapted PFR were carried out with saline tracer tests, validating the proposed adaptation. Six adapted PFR units were installed next to full-scale HSSFCW beds and fed with interstitial liquid pumped from two regions of planted and unplanted support media. Fourteen points were monitored along the system, covering carbon fractions, nitrogen and sulfate. The results indicate the method as a promising tool for investigations on CW support media, rhizosphere and open space for studies on CW modeling, respirometry, kinetic parameters, microbial communities, redox potential and plant influence on HSSFCW.

Performance comparison between two equal stabilization ponds operating with and without sludge layer.

Stabilization ponds are a highly appropriate system for treating sewage in small to medium size communities. However, sludge accumulation at the pond bottom occurs with the passage of time, reducing the net pond volume, which, in principle, could affect its performance. The objective of this paper is to compare the behaviour of two equal ponds in parallel treating the same flow of municipal wastewater from an upflow anaerobic sludge blanket reactor in Brazil. Each pond treated a population equivalent of around 125 inhabitants. One pond had approximately 40% of its net volume occupied by sludge after 11 years of operation, while the other pond had previously undergone complete desludging. The study covers the removal of biochemical oxygen demand (BOD), chemical oxygen demand (COD), suspended solids (SS), nitrogen fractions and coliforms. Owing to the presence of a sludge layer, the theoretical hydraulic retention time (HRT) was lower in the pond without sludge. For BOD, COD, SS and Escherichia coli there were no significant differences (Wilcoxon matched-pairs test) between both ponds. The pond without sludge had significantly better removal efficiencies in terms of total Kjeldahl nitrogen and ammonia-N. The sludge layer probably allowed the occurrence of removal mechanisms that compensated for the reduction caused in the HRT.