ABSTRACT
A mismatch between a circadian rhythm of blood-methotrexate level and that of the drug concentration in rat transplanted lymphosarcoma tissue was established. The drug proved 3 times more effective when given at the time of the day when its concentration in tumor tissue was likely to be the highest.
Subject(s)
Circadian Rhythm/physiology , Lymphoma, Non-Hodgkin/metabolism , Methotrexate/administration & dosage , Animals , Drug Screening Assays, Antitumor , Female , Injections, Subcutaneous , Lymphoma, Non-Hodgkin/drug therapy , Male , Methotrexate/pharmacokinetics , Neoplasm Transplantation , Rats , Rats, Wistar , Remission Induction , Time Factors , Tissue DistributionABSTRACT
In a transplanted lymphosarcoma of rats by means of Cosinor method we discovered rhythm of P32 inclusions with ultradian and circadian periods. In the course of tumor growth changes of spectrum of periods, amplitudes and acrophases of rhythms take place. These shifts are explained by the progression and clonal structure of neoplasms.
Subject(s)
Circadian Rhythm , Phosphorus/metabolism , Sarcoma, Experimental/metabolism , Animals , Female , Lymphoma, Non-Hodgkin/metabolism , Lymphoma, Non-Hodgkin/pathology , Male , Phosphorus Radioisotopes , Rats , Sarcoma, Experimental/pathologyABSTRACT
Rats bearing Pliss' lymphosarcoma revealed diurnal variations of body temperature. Cyclophosphamide and methotrexate were significantly less toxic when injected at peak temperatures than at minimal ones.
Subject(s)
Circadian Rhythm/drug effects , Cyclophosphamide/administration & dosage , Methotrexate/administration & dosage , Animals , Body Temperature/drug effects , Body Weight/drug effects , Cyclophosphamide/adverse effects , Cyclophosphamide/toxicity , Dose-Response Relationship, Drug , Drug Evaluation, Preclinical , Lymphoma, Non-Hodgkin/drug therapy , Methotrexate/adverse effects , Methotrexate/toxicity , Neoplasm Transplantation , Rats , Time FactorsSubject(s)
Hyperthermia, Induced , Lipopolysaccharides/therapeutic use , Lymphoma, Non-Hodgkin/radiotherapy , Metronidazole/therapeutic use , Radiation-Sensitizing Agents/therapeutic use , Animals , Combined Modality Therapy , Female , Lymphoma, Non-Hodgkin/metabolism , Male , Metronidazole/metabolism , Neoplasm Transplantation , Radiation-Sensitizing Agents/metabolism , RatsSubject(s)
Light , Neoplasms, Experimental/radiotherapy , Radiation Tolerance , Animals , Darkness , Lymphoma, Non-Hodgkin/radiotherapy , Male , Neoplasm Transplantation , Periodicity , Rats , Time FactorsABSTRACT
The total drug hyperthermia in the presence of metronidazole administration improves radiotherapeutic results in Pliss lymphosarcoma: tumor growth inhibition increases by 12.7-18.5%, tumor regression by 30.6-38.9%. A dose of 100 mg/kg of metronidazole combined with irradiation in the presence of moderate hyperthermia produces even a more noticeable radiosensitizing effect than a dose of 700 mg/kg without hyperthermia. A possibility of quite a high level of tumor radiosensitization with lower doses of metronidazole without toxic manifestations of the body was shown.
Subject(s)
Hyperthermia, Induced , Lymphoma, Non-Hodgkin/radiotherapy , Metronidazole/therapeutic use , Sulfisoxazole/therapeutic use , Animals , Combined Modality Therapy , Lymphoma, Non-Hodgkin/drug therapy , Neoplasm Transplantation , RatsSubject(s)
Darkness , Light , Neoplasms, Experimental/radiotherapy , Animals , Male , Radiation Tolerance , Rats , Time FactorsABSTRACT
Within a daily period maximal activity of ATPases and content of 32P in Pliss lymphosarcoma tissue was found in the samples taken at 15 or 21 p.m. and 3 a.m. The radioactivity in the tumor was minimal between 9 and 12 a.m. and 18 or 24 p.m. The alterations in Mg2+-ATPase activity in their magnitude and circadian rhythm coincided completely with the daily variations in content of radiophosphorus. Activities of Na+, K+- and HCO(3-)-ATPases exhibited maximal values at 15 p.m. and, especially between 3 and 6 a.m., i.e. these alterations were of two-peak shape without the peak at 21 p.m.
Subject(s)
Adenosine Triphosphatases/metabolism , Circadian Rhythm , Lymphoma, Non-Hodgkin/enzymology , Phosphorus Radioisotopes/metabolism , Animals , Ca(2+) Mg(2+)-ATPase , Lymphoma, Non-Hodgkin/metabolism , Male , Phosphorylation , Rats , Sarcoma, Experimental/enzymology , Sarcoma, Experimental/metabolism , Sodium-Potassium-Exchanging ATPase/metabolismABSTRACT
A possibility of radiosensitization of Pliss' lymphosarcoma and tumor RS-1 by using general hyperthermia with sulfazin was studied. Subcutaneous transfusion of sulfazin to rats caused a rise of the body temperature by 2-2.5 degrees C on the 2nd day after injection with the upkeep of hyperthermia for 3 days. A combined effect of sulfazin and irradiation enhanced the retardation of growth rate of tumors and increased their regression. The lifetime of animals increased significantly. A more noticeable postradiation suppression of mitotic activity was observed.
Subject(s)
Hyperthermia, Induced , Neoplasms, Experimental/therapy , Pyrogens/therapeutic use , Sulfisoxazole/therapeutic use , Animals , Female , Fever/chemically induced , Liver Neoplasms/radiotherapy , Liver Neoplasms/therapy , Lymphoma, Non-Hodgkin/radiotherapy , Lymphoma, Non-Hodgkin/therapy , Male , Neoplasm Transplantation , RatsSubject(s)
Botulism/epidemiology , Adolescent , Adult , Aged , Botulism/diagnosis , Botulism/therapy , Child , Female , Humans , Immunization, Passive/methods , Male , Middle Aged , RussiaABSTRACT
Under study was the effect of hydrocortisone injection and x-ray irradiation in a recipient on the development of a transplanted ovarian tumor in rats. The irradiation was shown to have a greater stimulating effect on the neoplastic growth than hydrocortisone.