Changes in Microbiota Composition during the Anaerobic Digestion of Macroalgae in a Three-Stage Bioreactor.

The use of microalgae as a raw material for biogas production is promising. Macroalgae were mixed with cattle manure, wheat straw, and an inoculant from sewage sludge. Mixing macroalgae with co-substrates increased biogas and methane yield. The research was carried out using a three-stage bioreactor. During biogas production, the dynamics of the composition of the microbiota in the anaerobic chamber of the bioreactor was evaluated. The microbiota composition at different organic load rates (OLRs) of the bioreactor was evaluated. This study also demonstrated that in a three-stage bioreactor, a higher yield of methane in biogas was obtained compared to a single-stage bioreactor. It was found that the most active functional pathway of methane biosynthesis is PWY-6969, which proceeds via the TCA cycle V (2-oxoglutarate synthase). Microbiota composition and methane yield depended on added volatile solids (VSadded). During the research, it was found that after reducing the ORL from 2.44 to 1.09 kg VS/d, the methane yield increased from 175.2 L CH4/kg VSadded to 323.5 L CH4/kg VSadded.

Changes in the Bacterial Communities of Biocomposites with Different Flame Retardants.

In today's world, the use of environmentally friendly materials is strongly encouraged. These materials derive from primary raw materials of plant origin, like fibrous hemp, flax, and bamboo, or recycled materials, such as textiles or residual paper, making them suitable for the growth of microorganisms. Here, we investigate changes in bacterial communities in biocomposites made of hemp shives, corn starch, and either expandable graphite or a Flovan compound as flame retardants. Using Next Generation Sequencing (NGS), we found that after 12 months of incubation at 22 °C with a relative humidity of 65%, Proteobacteria accounted for >99.7% of the microbiome in composites with either flame retardant. By contrast, in the absence of flame retardants, the abundance of Proteobacteria decreased to 32.1%, while Bacteroidetes (36.6%), Actinobacteria (8.4%), and Saccharobacteria (TM7, 14.51%) appeared. Using the increasing concentrations of either expandable graphite or a Flovan compound in an LB medium, we were able to achieve up to a 5-log reduction in the viability of Bacillus subtilis, Pseudomonas aeruginosa, representatives of the Bacillus and Pseudomonas genera, the abundance of which varied in the biocomposites tested. Our results demonstrate that flame retardants act on both Gram-positive and Gram-negative bacteria and suggest that their antimicrobial activities also have to be tested when producing new compounds.

The Impact of Microorganisms on the Performance of Linseed Oil and Tung Tree Oil Impregnated Composites Made of Hemp Shives and Corn Starch.

In this study, the performance characteristics of hemp shives impregnated with linseed oil and tung tree oil (HS)- and corn starch (CS)-based biocomposites containing flame retardants were evaluated before and after treatment with the mixture of bacterium Pseudomonas putida and fungus Rhizopus oryzae. Enzymatic activities and physical-mechanical properties such as water absorption, thickness swelling, compressive strength, and thermal conductivity were tested to evaluate the suitability of selected composites for thermal insulation purposes. In addition, electron microscopy was used to investigate the impact of microorganisms on the microstructure of the material. It was determined that the type of oil used for impregnation significantly affects the properties of biocomposites after 6 months of incubation with mixture of bacterium P. putida and fungus Rh. oryzae. Biocomposites impregnated with linseed oil and after treatment with a mixture of microorganisms had cellulase activity of 25 U/mL, endo ß-1-4-glucanase activity of 26 U/mL, lipase activity of 101 U/mL, only a 10% decrease in compressive strength, 50% higher short-term water absorption, unchanged swelling in thickness, and slightly decreased thermal conductivity compared to control biocomposites. At the same time, biocomposites with tung tree oil had a much more pronounced deterioration of the properties tested, cellulase activity of 28 U/mL, endo ß-1-4-glucanase activity of 37 U/mL, lipase activity of 91 U/mL, two times lower compressive strength and two times higher short-term water absorption, 2.5 times greater thickness swelling, and a slightly increased thermal conductivity. We conclude that linseed oil provides better protection against the action of microorganisms compared to impregnation with tung tree oil.

Impact of Cellulolytic Fungi on Biodegradation of Hemp Shives and Corn Starch-Based Composites with Different Flame-Retardants.

Biocomposite boards (BcBs) composed of hemp shives and corn starch are known as thermal insulating or structural building materials. Therefore, they must be stable during exploitation. However, BcBs are exposed to microorganisms present in the environment, and it is of great interest to investigate the biodegradation behaviour of these materials. This work identified microorganisms growing on BcBs that contain either Flovan CGN or expandable graphite as flame retardants and selected fungi such as Rhizopus oryzae and Aspergillus fumigatus to test the way they affect the materials of interest. For this purpose, the enzymatic activity of cellulases and amylases produced by these organisms were determined. In addition, the apparent density as well as compressive strength of the affected boards were evaluated. The results showed that apparent density and compressive strength deteriorated in BcB composition with the Flovan CGN flame retardant. At the same time, the level of deterioration was lower when the expandable graphite was used, suggesting that it also acts as an antimicrobial agent. A scanning electronic microscopy analysis was employed to monitor the growth of microorganisms in the BcBs. Such analysis demonstrated that, regardless of BcB composition, fungi easily penetrate into the middle layers of the material.

Microbial community dynamics in the mesophilic and thermophilic phases of textile waste composting identified through next-generation sequencing.

Composting is a promising source of mesophilic and thermophilic microorganisms directly involved in the decay of organic matter. However, there is a paucity of information related to bacterial and fungal diversity in compost and their enzymatic activities during the composting process. In this work, bacterial and fungal diversity during the mesophilic and thermophilic phases of textile waste composting was investigated as a way to explain the physical-chemical results obtained during the composting process. This was accomplished using a next-generation sequencing approach that targets either the 16S rRNA or ITS genomic regions of bacteria and fungi, respectively. It was observed that Proteobacteria, Bacteroidetes, and Actinobacteria were the dominant bacterial phyla present at the mesophilic phase but not at the thermophilic one. Composting textile waste exhibits a sustained thermophilic profile (above 55 °C) that usually precludes fungal activity. Nonetheless, the presence of fungi at the thermophilic phase was observed. Rozellomycota, Basidiomycota, and Ascomycota were the most dominant phyla during both composting phases. Such thermophilic fungi with great ability to decay organic matter could be isolated as pure cultures and used for the bioaugmentation of textile waste composting to achieve an advanced maturity level of textile waste compost.

The Effect of Different Plant Oil Impregnation and Hardening Temperatures on Physical-Mechanical Properties of Modified Biocomposite Boards Made of Hemp Shives and Corn Starch.

In this study, tung tree and linseed drying oils, as well as semi-drying hempseed oil, were analyzed as the protective coatings for biocomposite boards (BcB) made of hemp shives, corn starch binder, and the performance-enhancing additives. The hydrophobization coatings were formed at 40, 90, and 120 °C temperatures, respectively. The physical-mechanical properties such as the compressive strength, thermal conductivity, dimensional stability, water absorption, and swelling were tested. In addition, scanning electron microscopy (SEM) was employed for the analysis of the board microstructure to visualize the oil fills and impregnation in pores and voids. It was demonstrated that the compressive strength of oil-modified BcBs compared to uncoated BcBs (at 10% of relative deformation) increased by up to 4.5-fold and could reach up to 14 MPa, water absorption decreased up to 4-fold (from 1.34 to 0.37 kg/m2), swelling decreased up to 48% (from 8.20% to 4.26%), whereas the thermal conductivity remained unchanged with the thermal conductivity coefficient of around 0.085 W/m·K. Significant performance-enhancing properties were obtained due to the formation of a protective oil film when the tung tree oil was used.