Unravelling the effects of multiple stressors on diatom and macroinvertebrate communities in European river basins using structural and functional approaches.

Rivers suffer from more severe decreases in species diversity compared to other aquatic and terrestrial ecosystems due to a variety of pressures related to human activities. Species provide different roles in the functioning of the ecosystem, and their loss may reduce the capacity of the ecosystems to respond to multiple stressors. The effects on diversity will differ based on the type, combination and severity of stressors, as well as on the characteristics of the community composition and tolerance. Multiple trait-based approaches (MTBAs) can help to unravel the effects of multiple stressors on communities, providing a mechanistic interpretation, and, thus, complementing traditional biodiversity assessments using community structure. We studied the relationships between diversity indexes and trait composition of macroinvertebrate and diatom communities, as well as environmental variables that described the hydrological and geomorphological alterations and toxic pollution (pesticides and pharmaceuticals) of three different European river basins: the Adige, the Sava, and the Evrotas. These river basins can be considered representative cases of different situations in European freshwater systems. Hydrological variables were the main drivers determining the community structure and function in the rivers, for both diatoms and macroinvertebrates. For diatom communities, pharmaceutical active compound (PhAC) toxic units were also identified as a very important driver of diversity changes, explaining up to 57% of the variance in taxonomic richness. For macroinvertebrates, river geomorphology was an important driver of structural changes, particularly affecting Plecoptera richness. In addition, PhAC and pesticide toxic units were also identified as stressors for macroinvertebrate communities. MTBA provided a detailed picture of the effects of the stressors on the communities and confirmed the importance of hydrological variables in shaping the functional attributes of the communities.

Contribution to the improvement of diatom-based assessments of the ecological status of large rivers - The Sava River Case Study.

The Sava River Basin is a major drainage basin of southeastern Europe, significantly influenced by anthropogenic activities. Our study was focused on diatom communities as an indicator of the ecological status of running waters. We investigated over 937km of the Sava River at 19 sampling sites. Benthic diatom communities and 17 diatom indices were analyzed along with a large set of environmental parameters. CCA revealed that the most important elements along the spatial gradient were As and Si. Our results show that the species Navicula recens (Lange-Bert.) Lange-Bertalot and Eolimna minima (Grunow) Lange-Bertalot are very abundant at downstream localities where the highest concentrations of As were measured. The number of motile diatoms increased along the nutrient gradient, i.e. with Si availability. Correlations between diatom indices and selected environmental factors showed that temperature, As, Si and Fe are in significant negative correlation with most diatom indices. Analysis revealed the influence of As and metals in water on diatoms, although their concentrations did not exceed environmental standards. While our findings do not confirm that diatom indices reveal the intensity of pressures solely caused by nutrient and/or organic pollutants, they suggest that in moderately polluted large rivers benthic diatoms are good bioindicators of multiple pressures, and that diatom indices could serve as indicators of the level of overall degradation of an ecosystem.

Monitoring of DNA damage in haemocytes of freshwater mussel Sinanodonta woodiana sampled from the Velika Morava River in Serbia with the comet assay.

This study was undertaken to investigate the potential of the freshwater mussel Sinanodonta woodiana for detection of genotoxic pollution of the environment. Study was performed at two sites in the Velika Morava River, from May 2010 to February 2011. The alkaline comet assay on haemocytes was used, and the olive tail moment (OTM) was chosen as a measure of DNA damage. The specimens held on acclimation under controlled laboratory conditions for 10d were used as a control. Chemical analysis revealed the presence of phosphates and increased concentrations of zinc, copper and nickel at both sites during the entire sampling period. The values of OTM in mussels collected from the environment, significantly correlated with the concentration of zinc (r=0.6248), temperature (r=0.7006) and dissolved oxygen (r=0.7738). Seasonal variations in genotoxic response were observed, with the highest OTM values obtained during summer months. Preliminary results of the in vitro study indicated the effect of water temperature on genotoxic response to zinc and cadmium in S. woodiana suggesting that the presence of genotoxic pollutants during months with lower temperature could be under-estimated. Obtained results indicate that S. woodiana could be a valuable tool for active biomonitoring of aquatic environments and emphasizes the importance of seasonal genotoxic monitoring with this organism.