ABSTRACT
This study reports on the effect of PAF on sperm motility, fertilization and early embryo development in the sea urchin Strongylocentrotus intermedius. PAF proved to be the amplifier of sperm motility and fertilizing capability at 10(-8)-10(-9) M and was toxic at higher concentrations. BN 52201 did not counteract the PAF action. The advantages of using sea urchins for PAF research in embryology are discussed.
Subject(s)
Diterpenes , Models, Biological , Platelet Activating Factor/pharmacology , Sea Urchins/drug effects , Animals , Fertilization/drug effects , Ginkgolides , Lactones/pharmacology , Male , Platelet Activating Factor/antagonists & inhibitors , Platelet Activating Factor/toxicity , Sea Urchins/embryology , Sea Urchins/physiology , Sperm Motility/drug effectsABSTRACT
As reported earlier (Gulaya, N.M., Vaskovsky, V.E., Vystosky, M.V., Volkov, G.L., Govseeva, N.N. and Artemenko, I.P. (1988) Ukr. Biochim. J. 60, 58-63), N-acylphosphatidylethanolamines (NAPE) and products of their catabolism, N-acylethanolamines (NAE), are present in the lipids of neuroblastoma C1300 N18 undifferentiated cells. The present paper describes the distribution of NAE added to culture medium of differentiated cells and its effect on the fast sodium channels and some other membrane characteristics. It is shown that NAE inhibits the destroying action of veratridine on membranes.
Subject(s)
Cell Membrane/drug effects , Ethanolamines/pharmacology , Lipid Metabolism , Neuroblastoma/metabolism , Biological Transport/drug effects , Cell Membrane/metabolism , Cell Membrane Permeability/drug effects , Lysophospholipids/metabolism , Phosphatidylethanolamines/pharmacology , Sodium Channels/drug effects , Tumor Cells, Cultured/drug effects , Veratridine/antagonists & inhibitorsSubject(s)
Adaptation, Physiological , Ethers/analysis , Glycerol/analysis , Invertebrates/analysis , Animals , Climate , Japan , Species Specificity , Tropical ClimateSubject(s)
Chromatography, Thin Layer , Phospholipids/analysis , Indicators and Reagents , Methods , MolybdenumSubject(s)
Chromatography, Thin Layer , Phospholipids/analysis , Plasmalogens/analysis , Animals , Chemical Phenomena , Chemistry , StarfishABSTRACT
A simple procedure for preparing a stable stock reagent an working reagents for the detection and determination of phospholipids is proposed, and a simple, rapid and accurate thin-layer chromatographic technique is suggested. The results obtained for egg yolk phospholipids are presented as an example.
Subject(s)
Indicators and Reagents , Molybdenum , Phospholipids/analysis , Chromatography, Thin Layer , Egg Yolk/analysis , Female , HydrazinesSubject(s)
Invertebrates/enzymology , Phospholipases/analysis , Animals , Annelida/enzymology , Arthropods/enzymology , Chordata, Nonvertebrate/enzymology , Chromatography , Cnidaria/enzymology , Echinodermata/enzymology , Gonads/enzymology , Intestines/enzymology , Liver/enzymology , Mollusca/enzymology , Pancreas/enzymology , Phosphatidylcholines , Phosphatidylethanolamines , SphingomyelinsSubject(s)
Chromatography, Thin Layer , Lipids/analysis , Animals , Autoanalysis , Brain Chemistry , Glycolipids/analysis , Methods , Microchemistry , Phospholipids/analysis , Rats , SolventsSubject(s)
Indicators and Reagents , Molybdenum , Phospholipids/analysis , Chromatography, Thin Layer , Hydrazines , MethodsSubject(s)
Formaldehyde/analysis , Periodic Acid , Hot Temperature , Iodine , Mannitol , Methods , Oxidation-Reduction , Time FactorsABSTRACT
A simplified method for the preparation of the Dittmer-Lester spray for the detection of phospholipids on thin-layer chromatograms is described.
