ABSTRACT
BACKGROUND: Oxidative stress was implicated in the psoriasis disease development and may damage DNA leading to keratinocytes cell death. No serum biomarker was available for the oxidative DNA damage. OBJECTIVES: To evaluate the 8-OHdG (8-Hydroxy guanosine) as reliable biomarker for the oxidative stress in psoriatic patients with severity. METHODS: A total of 30 patients were considered for the study and graded according to the Psoriasis Area Severity Index (PASI) and 10 healthy controls. Blood was collected under aseptic condition, and serum was separated. Serum 8-OHdG and total antioxidant capacity was measured by competitive enzyme linked immunosorbent assay using `8-OHdG Check' and PAO kit (JaICA, Fukuroi City, Japan). RESULTS: The average serum 8-OHdG level in the control, mild, moderate and severe groups were 1.18 ± 0.93 ng/mL, 3.46 ± 0.82 ng/mL, 3.68 ± 0.67 ng/mL and 4.86 ± 1.7 ng/mL respectively. There was no significant difference in the average level of total antioxidant capacity of control, mild, moderate and severe groups, and the values presented were 295.88 ± 206 µmol/L, 1392.20 ± 225 µmol/L, 1199.57 ± 257 µmol/L and 1184.24 ± 207 µmol/L respectively. CONCLUSION: Serum 8-OHdG levels could be used as good biomarker for the early diagnosis of psoriasis and its management.
