ABSTRACT
The objective of the present work was to study conditions for isolation of ketorolac and diclofenac from biological fluids. A method of their extraction with a mixture of organic solvents has been developed and the conditions for the identification of these compounds are proposed with the use of high performance liquid chromatography (HPLC), UV spectroscopy, and gas chromatography with electron capture detection (GC/ECD). The possibilities of using HPLC, UV spectrometry, and GC/ECD for quantitative determination of ketorolac and diclofenac are illustrated.
Subject(s)
Diclofenac/blood , Ketorolac/blood , Chromatography, Gas/methods , Chromatography, High Pressure Liquid/methods , Humans , Sensitivity and Specificity , Spectrophotometry, Ultraviolet/methodsABSTRACT
Optimal conditions for the separation of diclofenac and ketorolac in the presence of other pharmaceutical products and narcotic substances are described with the help of gas and high performance liquid chromatography. The detection limits for individual compounds were established and the characteristic ions were identified. The possibility of application of IR- and UV-spectroscopy for the analysis of selected narcotic and non-narcotic analgesics was evaluated.
Subject(s)
Anti-Inflammatory Agents/analysis , Forensic Sciences/methods , Anti-Inflammatory Agents/chemistry , Chromatography, Gas/methods , Chromatography, High Pressure Liquid/methods , Mass Spectrometry/methods , Narcotics/analysis , Narcotics/chemistryABSTRACT
This work was devoted to the elucidation of conditions for isolation of ketorolac and diclofenac from biological fluids. A method is proposed for the extraction of these compounds from solutions with organic solvents at different pH values. Other methods permit to optimize identification of analytes by thin layer chromatography while the densitometric technique may be used for qualitative and quantitative analysis of their composition in biological fluids.
Subject(s)
Anti-Inflammatory Agents, Non-Steroidal/analysis , Body Fluids/chemistry , Chromatography, Thin Layer/methods , Diclofenac/analysis , Forensic Medicine , Ketorolac/analysis , Animals , Anti-Inflammatory Agents, Non-Steroidal/blood , Anti-Inflammatory Agents, Non-Steroidal/urine , Diclofenac/blood , Diclofenac/urine , Humans , Ketorolac/blood , Ketorolac/urine , Mice , Rats , Sensitivity and SpecificityABSTRACT
The conditions of butorphanol isolation from biological fluids were studied. The method of its extraction with the mix of organic solvents by pH 12 was proposed. How to identify butorphanol with the methods of thin-layer chromatography, ultraviolet spectrometry, high-performance liquid chromatography, gas chromatography with a detector of electron capture, chromato-mass spectrometry was developed. Possibility of use ultraviolet spectrometry for quantitative assessment of butorphanol was shown.
