Subject(s)
Bacteria/classification , Bacteria/isolation & purification , Nickel/metabolism , Soil Microbiology , Acidithiobacillus/classification , Acidithiobacillus/genetics , Acidithiobacillus/isolation & purification , Bacteria/genetics , DNA, Ribosomal/classification , DNA, Ribosomal/genetics , Industrial Waste , Leptospiraceae/classification , Leptospiraceae/genetics , Leptospiraceae/isolation & purification , Mining , Nickel/chemistry , PhylogenyABSTRACT
The sulfate-reducing strain 343T was isolated from ancient permafrost deposits in Siberia, Russia. Based on 16S rRNA gene sequence analyses, this strain was closely related to Desulfosporosinus species, showing 97.9 % 16S rRNA gene sequence similarity to Desulfosporosinus meridiei DSM 13257T, 97.6 % similarity to Desulfosporosinus auripigmenti DSM 13351T, 97.2 % similarity to Desulfosporosinus lacus DSM 15449T and 96.2 % similarity to Desulfosporosinus orientis DSM 765T. The strain was found to contain b-type cytochromes and to reduce only sulfate and thiosulfate using lactate as an electron donor but not sulfite, elemental sulfur, fumarate, nitrate or Fe(III). These data, considered in conjunction with DNA-DNA hybridization data, cell-wall chemotaxonomy and data on physiology, support recognition of strain 343T as representing a distinct and novel species within the genus Desulfosporosinus, namely Desulfosporosinus hippei sp. nov., with the type strain 343T (=DSM 8344T =VKM B-2003T).
Subject(s)
Ice Cover/microbiology , Peptococcaceae/classification , Sulfates/metabolism , Bacterial Typing Techniques , DNA, Bacterial/analysis , DNA, Ribosomal/analysis , Genes, rRNA , Genomics , Genotype , Molecular Sequence Data , Nucleic Acid Hybridization , Oxidation-Reduction , Peptococcaceae/genetics , Peptococcaceae/isolation & purification , Peptococcaceae/metabolism , Phenotype , Phylogeny , RNA, Ribosomal, 16S/genetics , Sequence Analysis, DNA , Siberia , Species SpecificityABSTRACT
The kinetics of conversion of sulfur compounds by Halothiobacillus neapolitanus DSM 15147 bacteria was studied in the presence of steel samples. It was shown that the presence of steel altered the known pathway of sulfur compound oxidation by thiobacteria. Production of atomic hydrogen via the interaction between biogenic sulfuric acid and steel enhanced secondary production of intermediates and decreased the content of sulfate produced previously. The process was accompanied by pH elevation and continuation of intense growth of the thiobacterium culture. Thiobacteria formed a corrosive medium, which caused metal destruction. The protective properties of anticorrosive coatings 225 LS and 640 mk were tested. It was shown that these coatings protected steel from the destructive effect of biogenic sulfuric acid.
Subject(s)
Halothiobacillus/metabolism , Steel/chemistry , Sulfur Compounds/metabolism , Corrosion , Culture Media, Conditioned/metabolism , Culture Media, Conditioned/pharmacology , Halothiobacillus/growth & development , Hydrogen-Ion Concentration , Oxidation-Reduction , Sulfuric Acids/adverse effects , Sulfuric Acids/metabolism , Surface-Active AgentsABSTRACT
Bacteria belonging to different taxonomic and physiological groups (members of the genera Pseudomonas, Brevibacterium, Rhodopseudomonas, and Lactococcus) are able to form intracellular cobalt- and chromium-containing magnetic inclusions. The paper deals with the structure and the intracellular localization of these inclusions and their similarity to the known noncrystalline iron-containing magnetic inclusions. The possible biological role of the magnetic inclusions is discussed.
