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1.
Nat Cell Biol ; 10(12): 1477-83, 2008 Dec.
Article in English | MEDLINE | ID: mdl-19029908

ABSTRACT

Neurons and cancer cells use glucose extensively, yet the precise advantage of this adaptation remains unclear. These two seemingly disparate cell types also show an increased regulation of the apoptotic pathway, which allows for their long-term survival. Here we show that both neurons and cancer cells strictly inhibit cytochrome c-mediated apoptosis by a mechanism dependent on glucose metabolism. We report that the pro-apoptotic activity of cytochrome c is influenced by its redox state and that increases in reactive oxygen species (ROS) following an apoptotic insult lead to the oxidation and activation of cytochrome c. In healthy neurons and cancer cells, however, cytochrome c is reduced and held inactive by intracellular glutathione (GSH), generated as a result of glucose metabolism by the pentose phosphate pathway. These results uncover a striking similarity in apoptosis regulation between neurons and cancer cells and provide insight into an adaptive advantage offered by the Warburg effect for cancer cell evasion of apoptosis and for long-term neuronal survival.


Subject(s)
Apoptosis , Cytochromes c/metabolism , Glucose/metabolism , Neurons/cytology , Neurons/metabolism , Animals , Apoptosis/drug effects , Cell Line, Tumor , Cytoprotection/drug effects , Humans , Mice , Mice, Inbred C57BL , Nerve Growth Factor/pharmacology , Oxidation-Reduction/drug effects , Pentose Phosphate Pathway/drug effects , Sympathetic Nervous System/cytology
2.
Proc Natl Acad Sci U S A ; 104(52): 20820-5, 2007 Dec 26.
Article in English | MEDLINE | ID: mdl-18093951

ABSTRACT

Brain tumors are typically resistant to conventional chemotherapeutics, most of which initiate apoptosis upstream of mitochondrial cytochrome c release. In this study, we demonstrate that directly activating apoptosis downstream of the mitochondria, with cytosolic cytochrome c, kills brain tumor cells but not normal brain tissue. Specifically, cytosolic cytochrome c is sufficient to induce apoptosis in glioblastoma and medulloblastoma cell lines. In contrast, primary neurons from the cerebellum and cortex are remarkably resistant to cytosolic cytochrome c. Importantly, tumor tissue from mouse models of both high-grade astrocytoma and medulloblastoma display hypersensitivity to cytochrome c when compared with surrounding brain tissue. This differential sensitivity to cytochrome c is attributed to high Apaf-1 levels in the tumor tissue compared with low Apaf-1 levels in the adjacent brain tissue. These differences in Apaf-1 abundance correlate with differences in the levels of E2F1, a previously identified activator of Apaf-1 transcription. ChIP assays reveal that E2F1 binds the Apaf-1 promoter specifically in tumor tissue, suggesting that E2F1 contributes to the expression of Apaf-1 in brain tumors. Together, these results demonstrate an unexpected sensitivity of brain tumors to postmitochondrial induction of apoptosis. Moreover, they raise the possibility that this phenomenon could be exploited therapeutically to selectively kill brain cancer cells while sparing the surrounding brain parenchyma.


Subject(s)
Apoptotic Protease-Activating Factor 1/metabolism , Brain Neoplasms/metabolism , Brain/metabolism , Cytochromes c/metabolism , Gene Expression Regulation, Neoplastic , Apoptosis , Astrocytoma/metabolism , Caspases/metabolism , Cytochromes c/chemistry , E2F1 Transcription Factor/chemistry , Humans , Medulloblastoma/metabolism , Neurons/metabolism , Oligonucleotide Array Sequence Analysis , Promoter Regions, Genetic , Transcription, Genetic
3.
J Cell Biol ; 171(6): 925-30, 2005 Dec 19.
Article in English | MEDLINE | ID: mdl-16344307

ABSTRACT

Overexpression studies have identified X-linked inhibitor of apoptosis protein (XIAP) as a potent inhibitor of caspases. However, the exact function of endogenous XIAP in regulating mammalian apoptosis is less clear. Endogenous XIAP strictly regulates cytochrome c-dependent caspase activation in sympathetic neurons but not in many mitotic cells. We report that postmitotic cardiomyocytes, unlike fibroblasts, are remarkably resistant to cytosolic microinjection of cytochrome c. The cardiomyocyte resistance to cytochrome c is mediated by endogenous XIAP, as XIAP-deficient cardiomyocytes die rapidly with cytosolic cytochrome c alone. Importantly, we found that cardiomyocytes, like neurons, have markedly reduced Apaf-1 levels and that this decrease in Apaf-1 is directly linked to the tight regulation of caspase activation by XIAP. These data identify an important function of XIAP in cardiomyocytes and point to a striking similarity in the regulation of apoptosis in postmitotic cells.


Subject(s)
Apoptosis/drug effects , Intracellular Signaling Peptides and Proteins/metabolism , Myocytes, Cardiac/metabolism , Proteins/metabolism , X-Linked Inhibitor of Apoptosis Protein/metabolism , Animals , Apoptotic Protease-Activating Factor 1 , Caspases/metabolism , Cells, Cultured , Cytochromes c/metabolism , Cytochromes c/pharmacology , Cytosol/metabolism , Mice , Microinjections , Microscopy, Fluorescence , Myocytes, Cardiac/drug effects , Myocytes, Cardiac/enzymology , Rats , Time Factors , Transfection , X-Linked Inhibitor of Apoptosis Protein/pharmacology
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