ABSTRACT
Swine eperythrozoonosis or porcine hemoplasmosis is an infectious disease caused mainly by Mycoplasma suis and is distributed worldwide. This study investigated the occurrence of porcine hemothropic mycoplasmas (PHMs) in fetuses and sows with reproductive failure. Two hundred and seventy-six samples (80 sows' blood and 196 fetal tissue samples) from 27 farms with reproductive disorders were evaluated. The PHMs DNA was detected in 15 out of 80 (18.7%) sows but it was not detected in the fetuses. The bacterial load ranged from 1.32â¯×â¯102 to 2.61â¯×â¯105 copies/µL. From the 27 tested herds, 11 (40.7%) showed at least one positive sow per farm. The majority of the reproductive problems observed in PMHs positive sows were stillborn fetuses (46.7%) and stillborn associated with fetal mummification (26.7%). So, we evidenced that porcine hemoplasmas circulate among sows in Brazilian herds, however, its real impact on reproductive problems remains unknown.
ABSTRACT
Yersinia enterocolitica is a foodborne pathogen and pigs are the main reservoir of it in their tonsils. As Brazil is a large producer and exporter of pork meat and information regarding this pathogen is still quite scarce, this study aimed at evaluating the direct detection of Y. enterocolitica followed by pathogenic Y. enterocolitica (PYE) determination in tonsils of slaughtered pigs. For this purpose, 400 pig tonsils were collected from 15 farms in four federally certified slaughterhouses in Southern Brazil. Initially, samples were screened using conventional PCR targeting of the 16sRNA gene, followed by multiplex PCR (mPCR) in order to detect three virulence genes (ail, yadA, and virF) and quantitative real-time PCR (qPCR) for the detection of the ail gene. One hundred and one (25.2%) of the samples tested positive for the 16sRNA gene. However, a PYE was detected in one out of the 101 Y. enterocolitica positive samples. The three virulence genes were determined by mPCR and confirmed by partial DNA sequencing. Thus, a significant occurrence of Y. enterocolitica was observed in pig tonsils from federally inspected slaughterhouses in Brazil, although the presence of pathogenic strains was quite low.
Subject(s)
Palatine Tonsil/microbiology , Red Meat/microbiology , Swine Diseases/microbiology , Swine/microbiology , Yersinia Infections/veterinary , Yersinia enterocolitica , Abattoirs , Animals , Brazil/epidemiology , Real-Time Polymerase Chain Reaction , Swine Diseases/epidemiology , Virulence , Yersinia Infections/epidemiologyABSTRACT
O presente estudo foi realizado em sete propriedades no Planalto Catarinense, os quais apresentavam algum histórico de transtorno reprodutivo. Foram coletadas 101 amostras de urinas de matrizes em produçao. Foi colhida uma amostra da primeira urina espontânea da manha e foi feito imunofluorescência indireta a partir de esfregaços do sedimento urinário. As lâminas foram avaliadas e classificadas como negativas (ausência de bastonete ou raros bastonetes com fluorescência leve) e positiva (presença de bastonetes com fluorescência celular ou nas bordas evidente e brilhante). Dos 101 esfregaços examinados, 17 (l6,8 porcento) foram positivos para a presença do Actinomyces suis (A. suis), observando-se uma variaçao de 14 a 80 porcento, sendo as mesmas procedentes de quatro propriedades. Estes resultados encontrados mostram que em granjas com problemas reprodutivos o A. Suis pode ser um dos agentes etiológicos envolvidos no desencadeamento do problema.
