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1.
J Pharm Sci ; 112(7): 1749-1762, 2023 07.
Article in English | MEDLINE | ID: mdl-37142122

ABSTRACT

The workshop "Drug Permeability - Best Practices for Biopharmaceutics Classification System (BCS) Based Biowaivers" was held virtually on December 6, 2021, organized by the University of Maryland Center of Excellence in Regulatory Science and Innovation (M-CERSI), and the Food and Drug Administration (FDA). The workshop focused on the industrial, academic, and regulatory experiences in generating and evaluating permeability data, with the aim to further facilitate implementation of the BCS and efficient development of high-quality drug products globally. As the first international permeability workshop since the BCS based biowaivers was finalized as the ICH M9 guideline, the workshop included lectures, panel discussions, and breakout sessions. Lecture and panel discussion topics covered case studies at IND, NDA, and ANDA stages, typical deficiencies relating to permeability assessment supporting BCS biowaiver, types of evidence that are available to demonstrate high permeability, method suitability of a permeability assay, impact of excipients, importance of global acceptance of permeability methods, opportunities to expand the use of biowaivers (e.g. non-Caco-2 cell lines, totality-of-evidence approach to demonstrate high permeability) and future of permeability testing. Breakout sessions focused on 1) in vitro and in silico intestinal permeability methods; 2) potential excipient effects on permeability and; 3) use of label and literature data to designate permeability class.


Subject(s)
Biopharmaceutics , Research Report , Pharmaceutical Preparations , Biopharmaceutics/methods , Therapeutic Equivalency , Excipients , Permeability , Solubility
2.
Brain Res ; 876(1-2): 17-21, 2000 Sep 08.
Article in English | MEDLINE | ID: mdl-10973588

ABSTRACT

In normotensive Wistar rats, systemic administration of exogenous ouabain for 10 days or more induces hypertension, presumably through central mechanisms. To identify which neuronal populations may be involved, we assessed Fos-like immunoreactivity (FLI) using an antibody that recognizes the protein products of the fos family comprising Fos, Fos B, Fra 1 and Fra 2, thus enabling detection of chronic neuronal activation. Young Wistar rats received s.c. infusions of either ouabain (50 microg/day) or saline for 7 or 14 days. At the end of the experimental period, mean arterial pressure (MAP) was assessed. In a separate set of rats FLI was detected immunohistochemically and quantified in cardiovascular and osmo-regulating centers. Resting MAP in ouabain-treated rats was significantly higher than in control rats at 14 but not at 7 days (125+/-4 vs. 101+/-6, P<0.05 and 102+/-4 vs. 98+/-6 (not significant), respectively). Within the supraoptic nucleus, ouabain induced significant increases in FLI compared with control rats at 14 days (9+/-2 vs. 2+/-2, P<0.05) but not at 7 days. Within the locus ceruleus, FLI was only detectable in rats that received ouabain infusions for 14 days but not in other groups of rats. Ouabain treatment did not induce significant changes in FLI within other areas. These results demonstrate that chronic s.c. ouabain infusion only increases neuronal FLI in the supraoptic nucleus and locus ceruleus where increases in FLI parallel the increase in blood pressure.


Subject(s)
Hypertension/chemically induced , Hypertension/metabolism , Neurons/metabolism , Ouabain , Proto-Oncogene Proteins c-fos/metabolism , Animals , Blood Pressure/drug effects , Hypertension/physiopathology , Immunohistochemistry , Injections, Subcutaneous , Locus Coeruleus/metabolism , Male , Ouabain/pharmacology , Rats , Rats, Wistar , Reference Values , Supraoptic Nucleus/metabolism
3.
Am J Physiol ; 276(1): H63-70, 1999 01.
Article in English | MEDLINE | ID: mdl-9887018

ABSTRACT

To examine the role of the ventral anteroventral third ventricle (vAV3V) in the hypertension induced by chronic subcutaneous ouabain and intracerebroventricular hypertonic saline, neurons in this area were destroyed by microinjection of an excitotoxin, ibotenic acid. Sham-operated or lesioned Wistar rats were administered ouabain (50 microgram/day) or placebo for 3 wk from subcutaneously implanted controlled release pellets or artificial cerebrospinal fluid (CSF) or CSF containing 0.8 mol/l NaCl (5 microliter/h) infused intracerebroventricularly for 2 wk. At the end of the experiment, mean arterial pressure (MAP) and heart rate at rest and in response to ganglionic blockade by intravenous hexamethonium (30 mg/kg) were assessed. In rats infused with hypertonic saline, responses to air jet stress were also assessed. Baseline MAP in sham-operated rats receiving intracerebroventricular hypertonic saline or subcutaneous ouabain was significantly higher than in control rats (115 +/- 1 vs. 97 +/- 3 and 121 +/- 3 vs. 103 +/- 3 mmHg, respectively). vAV3V lesions abolished the increase in MAP elicited by chronic infusion of hypertonic saline or administration of ouabain. Sham-operated rats treated with hypertonic saline or ouabain exhibited significantly enhanced decreases in MAP to hexamethonium, but lesioned rats did not. Rats infused with hypertonic saline demonstrated enhanced responses to air jet stress that were similar in sham-operated and lesioned rats. These results demonstrate that neurons in the vAV3V are essential for the hypertension induced by intracerebroventricular hypertonic saline and subcutaneous ouabain, possibly by increasing sympathetic tone. Cardiovascular responses to air jet stress appear not to be mediated by the vAV3V.


Subject(s)
Cerebral Ventricles/physiology , Hypertension/chemically induced , Ouabain , Sodium , Animals , Cerebral Ventricles/drug effects , Cerebral Ventricles/pathology , Drug Implants , Excitatory Amino Acid Agonists/pharmacology , Ibotenic Acid/pharmacology , Injections, Intraventricular , Male , Microinjections , Ouabain/administration & dosage , Rats , Rats, Wistar , Saline Solution, Hypertonic/administration & dosage , Sodium/administration & dosage
4.
Am J Physiol ; 274(4): H1269-76, 1998 04.
Article in English | MEDLINE | ID: mdl-9575931

ABSTRACT

Both brain ouabain-like activity ("ouabain") and brain angiotensin II (ANG II) contribute to the sympathoexcitatory and pressor responses to high sodium intake in spontaneously hypertensive (SHR) and Dahl salt-sensitive (Dahl S) rats. To assess whether increases in cerebrospinal fluid (CSF) sodium can mimic this pattern of changes, Wistar rats were chronically infused with artificial CSF (aCSF) or sodium-rich aCSF (0.8 or 1.2 M sodium) intracerebroventricularly through osmotic minipumps for 14 days. Sodium-rich aCSF (0.8 M) was also infused intracerebroventricularly for 2 wk concomitantly with either antibody Fab fragments that bind ouabain and related steroids with high affinity, gamma-globulins as control (200 micrograms/day for both), or the AT1 blocker losartan (1 mg.kg-1.day-1). Sodium-rich aCSF increased CSF sodium from 146 +/- 2 to 152 +/- 2 (0.8 M) and 160 +/- 3 (1.2 M) mmol/l, and increased brain "ouabain" in the hypothalamus, pituitary, and pons. In conscious rats, sodium-rich aCSF increased baseline mean arterial pressure (MAP), enhanced MAP, heart rate (HR), and renal sympathetic nerve activity (RSNA) responses to intracerebroventricular alpha 2-adrenoceptor agonist guanabenz and air stress, and desensitized arterial and cardiopulmonary baroreflex control of HR and RSNA. These effects were largely prevented by intracerebroventricular Fab fragments or losartan. Thus, in Wistar rats, both brain "ouabain" and the brain renin-angiotensin system contribute to sympathoexcitation, impairment of baroreflexes, and hypertension caused by chronically increased CSF sodium. The similar patterns of changes caused by CSF sodium in Wistar rats and by high sodium intake in SHR and Dahl S rats indicate that if high sodium intake increases central sodium, such changes may contribute to sympathoexcitation and hypertension.


Subject(s)
Angiotensin II/metabolism , Brain/metabolism , Ouabain/metabolism , Sodium/metabolism , Sympathetic Nervous System/physiology , Adrenergic alpha-Agonists/pharmacology , Animals , Arteries/physiology , Baroreflex/physiology , Guanabenz/pharmacology , Heart/physiology , Hemodynamics/drug effects , Injections, Intraventricular , Kidney/blood supply , Kidney/innervation , Lung/physiology , Male , Rats , Rats, Wistar , Sodium/cerebrospinal fluid , Stress, Physiological/physiopathology , Sympathetic Nervous System/drug effects
5.
Brain Res ; 749(1): 157-60, 1997 Feb 21.
Article in English | MEDLINE | ID: mdl-9070643

ABSTRACT

To clarify the role of neurones in the anteroventral third ventricle (AV3V) area in cardiovascular responses to CSF sodium, ouabain and angiotensin II (ANG II), we employed excitotoxic lesions of the ventral AV3V (vAV3V). In conscious lesioned Wistar rats with systemic vasopressin blockade, pressor and tachycardiac responses to intracerebroventricular (i.c.v.) artificial CSF containing 0.3 M NaCl or ouabain were significantly attenuated by 26-32% whereas responses to ANG II were not affected. Thus, in rats with systemic blockade of vasopressin mechanisms, the vAV3V region partially mediates acute pressor responses to i.c.v. sodium and ouabain but not to ANG II.


Subject(s)
Angiotensin II/pharmacology , Blood Pressure/drug effects , Cerebral Ventricles/pathology , Encephalitis/chemically induced , Ouabain/pharmacology , Sodium/pharmacology , Angiotensin II/administration & dosage , Animals , Cardiotonic Agents/administration & dosage , Cardiotonic Agents/pharmacology , Heart Rate/drug effects , Injections, Intraventricular , Male , Ouabain/administration & dosage , Rats , Rats, Wistar , Sodium/cerebrospinal fluid
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