Breaking the Law: Is It Correct to Use the Converse Bergmann Rule in Ceroglossus chilensis? An Overview Using Geometric Morphometrics.

The converse Bergmann's rule is a pattern of body size variation observed in many ectothermic organisms that contradicts the classic Bergmann's rule and suggests that individuals inhabiting warmer climates tend to exhibit larger body sizes compared to those inhabiting colder environments. Due to the thermoregulatory nature of Bergmann's rule, its application among ectotherms might prove to be more complicated, given that these organisms obtain heat by absorbing it from their habitat. The existence of this inverse pattern therefore challenges the prevailing notion that larger body size is universally advantageous in colder climates. Ceroglossus chilensis is a native Chilean beetle that has the largest latitudinal range of any species in the genus, from 34.3° S to 47.8° S. Within Chile, it continuously inhabits regions extending from Maule to Aysen, thriving on both native and non-native forest species. Beyond their remarkable color variation, populations of C. chilensis show minimal morphological disparity, noticeable only through advanced morphological techniques (geometric morphometrics). Based on both (1) the "temperature-size rule", which suggests that body size decreases with increasing temperature, and (2) the reduced resource availability in high-latitude environments that may lead to smaller body sizes, we predict that C. chilensis populations will follow the converse Bergmann's rule. Our results show a clear converse pattern to the normal Bergmann rule, where smaller centroid sizes were found to be measured in the specimens inhabiting the southern areas of Chile. Understanding the prevalence of the converse Bergmann's rule for ectotherm animals and how often this rule is broken is of utmost importance to understand the underlying mechanisms allowing organisms to adapt to different environments and the selective pressures they face.

Geographic variation in the matching between call characteristics and tympanic sensitivity in the Weeping lizard.

Effective communication requires a match among signal characteristics, environmental conditions, and receptor tuning and decoding. The degree of matching, however, can vary, among others due to different selective pressures affecting the communication components. For evolutionary novelties, strong selective pressures are likely to act upon the signal and receptor to promote a tight match among them. We test this prediction by exploring the coupling between the acoustic signals and auditory sensitivity in Liolaemus chiliensis, the Weeping lizard, the only one of more than 285 Liolaemus species that vocalizes. Individuals emit distress calls that convey information of predation risk to conspecifics, which may respond with antipredator behaviors upon hearing calls. Specifically, we explored the match between spectral characteristics of the distress calls and the tympanic sensitivities of two populations separated by more than 700 km, for which previous data suggested variation in their distress calls. We found that populations differed in signal and receptor characteristics and that this signal variation was explained by population differences in body size. No precise match occurred between the communication components studied, and populations differed in the degree of such correspondence. We suggest that this difference in matching between populations relates to evolutionary processes affecting the Weeping lizard distress calls.

Persistence and size of seasonal populations on a consumer-resource relationship depends on the allocation strategy toward life-history functions.

The long-term ecological dynamics of a population inhabiting a seasonal environment is analyzed using a semi-discrete or impulsive system to represent the consumer-resource interaction. The resource corresponds to an incoming energy flow for consumers that is allocated to reproduction as well as to maintenance in each non-reproductive season. The energy invested in these life-history functions is used in reproductive events, determining the size of the offspring in each reproductive season. Two long-term dynamic patterns are found, resulting in either the persistence or the extinction of the population of consumers. In addition, our model indicates that only one energy allocation strategy provides an optimal combination between individual consumption and long-term population size. The current study contributes to the understanding of how the individual-level and the population-level are interrelated, exhibiting the importance of incorporating phenotypic traits in population dynamics.

The acoustic adaptation hypothesis in a widely distributed South American frog: Southernmost signals propagate better.

Animal communication occurs in environments that affect the properties of signals as they propagate from senders to receivers. We studied the geographic variation of the advertisement calls of male Pleurodema thaul individuals from eight localities in Chile. Furthermore, by means of signal propagation experiments, we tested the hypothesis that local calls are better transmitted and less degraded than foreign calls (i.e. acoustic adaptation hypothesis). Overall, the advertisement calls varied greatly along the distribution of P. thaul in Chile, and it was possible to discriminate localities grouped into northern, central and southern stocks. Propagation distance affected signal amplitude and spectral degradation in all localities, but temporal degradation was only affected by propagation distance in one out of seven localities. Call origin affected signal amplitude in five out of seven localities and affected spectral and temporal degradation in six out of seven localities. In addition, in northern localities, local calls degraded more than foreign calls, and in southern localities the opposite was observed. The lack of a strict optimal relationship between signal characteristics and environment indicates partial concordance with the acoustic adaptation hypothesis. Inter-population differences in selectivity for call patterns may compensate for such environmental constraints on acoustic communication.

Individual and sex distinctiveness in bark calls of domestic chinchillas elicited in a distress context.

Animals obtain information about their social environment by means of communication signals, which provide relevant subtle cues for individual recognition. An important requisite for this process is the existence of larger between- than within-emitter signal variation. Acoustic signals are complex traits susceptible of variation in their spectral and temporal components, implying that signal distinctiveness can result from differences in single or various acoustic components. In this study, domestic chinchillas were induced to vocalize in a distress context to describe the acoustic characteristics of the bark calls, and to determine features that denote the potential value of this vocalization for individual and/or sexual recognition. The results demonstrate that the variation in spectral and temporal components of the bark calls of chinchillas elicited under a distress context is larger between than within individuals, suggesting the potential of these signals for distinctiveness between individual signalers, although the potential of this call type for sex distinctiveness is quite limited. These results combined with previous studies on auditory capabilities of chinchillas contribute to position this rodent as a valuable model species for studying auditory-vocal interactions.

Lack of phonotactic preferences of female frogs and its consequences for signal evolution.

Sexual selection is one of the main evolutionary forces that drive signal evolution. In previous studies, we have found out that males of Pleurodema thaul, a frog with an extensive latitudinal distribution in Chile, emits advertisement calls that show remarkable variation among populations. In addition, this variation is related to intense inter-male acoustic competition (intra-sexual selection) occurring within each population. However, the extent to which female preferences contribute to the signal divergence observed is unclear. To study the responsiveness of females in each population, we stimulated females with synthetic calls designed with the acoustic structure of their own population and subsequently responsive females were subjected to a two-choice experiment, where they were stimulated with synthetic calls of their own population versus a call of a foreign population. Females do not show phonotactic preferences for calls of their own or foreign populations as measured with both linear and circular variables. The lack of phonotactic preferences suggests an absence of participation of inter-sexual selection processes in the divergence of the acoustic signals of P. thaul, highlighting the importance of intra-sexual selection for the evolution of these signals. These results concur with studies in other vertebrates emphasizing the relevance of interactions among males for the evolution of acoustic communication systems.

Geographic variation in acoustic communication in anurans and its neuroethological implications.

Geographic variation of traits may represent the first step for evolutionary divergence potentially leading to speciation. Signals are behavioral traits of particular interest for the study of variation at a geographic scale. The anuran acoustic communication system represents an excellent model for studies of this kind, because their vocalizations play a main role in reproduction and the extant variation in this system may determine the evolution of this group. This review is committed to studies on geographic variation of acoustic communication systems in anurans, focusing on temporal and spectral characteristics of signals, environmental constraints affecting them and sound producing and receiving organs. In addition to the review of the literature on these topics, I highlight the deficit of investigation in some areas and propose alternative directions to overcome these drawbacks. Further, I propose the four-eyed frog, Pleurodema thaul, as an excellent model system to study geographic variation using a wide spectrum of approaches.

Divergence of acoustic signals in a widely distributed frog: relevance of inter-male interactions.

Divergence of acoustic signals in a geographic scale results from diverse evolutionary forces acting in parallel and affecting directly inter-male vocal interactions among disjunct populations. Pleurodema thaul is a frog having an extensive latitudinal distribution in Chile along which males' advertisement calls exhibit an important variation. Using the playback paradigm we studied the evoked vocal responses of males of three populations of P. thaul in Chile, from northern, central and southern distribution. In each population, males were stimulated with standard synthetic calls having the acoustic structure of local and foreign populations. Males of both northern and central populations displayed strong vocal responses when were confronted with the synthetic call of their own populations, giving weaker responses to the call of the southern population. The southern population gave stronger responses to calls of the northern population than to the local call. Furthermore, males in all populations were stimulated with synthetic calls for which the dominant frequency, pulse rate and modulation depth were varied parametrically. Individuals from the northern and central populations gave lower responses to a synthetic call devoid of amplitude modulation relative to stimuli containing modulation depths between 30-100%, whereas the southern population responded similarly to all stimuli in this series. Geographic variation in the evoked vocal responses of males of P. thaul underlines the importance of inter-male interactions in driving the divergence of the acoustic traits and contributes evidence for a role of intra-sexual selection in the evolution of the sound communication system of this anuran.