DNA metabarcoding, diversity partitioning and null models reveal mechanisms of seasonal trophic specialization in a Mediterranean warbler.

Optimal Foraging Theory (OFT) predicts that a population's trophic niche expansion should occur in periods of food scarcity as individuals begin to opportunistically exploit sub-optimal food items. However, the Niche Variation Hypothesis (NVH) posits that niche widening may result from increased among-individual differentiation due to food partitioning to avoid competition. We tested these hypotheses through a DNA metabarcoding study of the Sardinian Warbler (Curruca melanocephala) diet over a year. We used null models and the decomposition of beta diversity on among-individual dietary differentiation to infer the mechanisms driving the population's niche variation. Warblers fed frequently on berries, with a peak in late summer and, to a lesser extent, in autumn. Their diet also included a wide range of arthropods, with their prevalence varying among seasons. Consistent with OFT, the population's niche width was narrower in spring/summer when the population was strongly specialized in berries. In winter, the population's niche expanded, possibly reflecting seasonal declines in food abundance. As predicted by NVH, among-individual differentiation tended to be higher in winter, but this was mainly due to increased differences in dietary richness rather than to the partitioning of resources. Overall, our results suggest that within-individual niche does not increase in lean periods, and instead, individuals adopt either a more opportunistic or more specialized foraging strategy. Increased competition in periods of scarcity may help explain such patterns, but instead of showing increased food partitioning as expected from NVH, it may reflect OFT mechanisms on individuals with differential competitive ability to access better food resources.

Maternal care according to offspring sex and maternal physical condition in mantled howler monkeys (Alouatta palliata).

The Trivers-Willard hypothesis (TWH) posits that maternal care will be biased in favor of the sex that provides the greatest fitness returns per unit of investment, depending on maternal physical condition. Our aim was to examine the TWH in mantled howler monkeys living at Los Tuxtlas (Veracruz, Mexico). The biological attributes of mantled howler monkeys (Alouatta palliata) meet the assumptions of TWH better than those of other explanations, so we expected that females in better physical condition should bias maternal care toward sons, whereas mothers in worse physical condition should bias care toward daughters. Between December 2017 and March 2019, we studied mother-infant interactions in 20 dyads with focal-animal sampling and continuous recording (N = 204 h). We performed genetic analysis to determine offspring sex (N = 7 daughters and 13 sons) and measured C-peptide in urine samples of mothers to assess their physical condition (N = 46 samples). Mothers in better physical condition spent less time in contact with their sons but more time in contact with their daughters. For proximity behavior, mothers in better physical condition spent more time near their sons and less time near their daughters. These results suggest a bias in maternal care towards daughters, contrary to our predictions. In light of current models of maternal investment, our results support that mothers obtain higher fitness returns through daughters.