Surface Acoustic Wave-Driven Enhancement of Enzyme-Linked Immunosorbent Assays: ELISAW.

Enzyme-linked immunosorbent assays (ELISAs) are widely used in biology and clinical diagnosis. Relying on antigen-antibody interaction through diffusion, the standard ELISA protocol can be time-consuming, preventing its use in rapid diagnostics. We present a time-saving and more sensitive ELISA without changing the standard setup and protocol, using surface acoustic waves (SAWs) to enhance performance. Each step of the assay, from the initial antibody binding onto the walls of the well plate to the target analyte molecules' binding for detection─except, notably, for the blocking step─is improved principally via acoustic streaming-driven advection. Using SAWs, the time required for one step of an example ELISA is reduced from 60 to 15 min to achieve the same binding amount. By extending the duration of SAW exposure to 20 min, the sensitivity can be significantly improved over the 60 min, 35 °C ELISA without SAWs. It is also possible to confer beneficial improvements to bead-based ELISA by combining it with SAWs to further reduce the time required for binding to 2 min. By significantly increasing the speed of ELISA, its utility may be improved for a wide range of point-of-care diagnostics applications.

Estrium Whey induced hepatitis in a patient with metastatic breast cancer: Case report.

Estrium Whey is an alternative nutritional support therapy for women. It's enhanced with specific nutrients including phytoestrogens, folate, antioxidants and fiber to support healthy estrogen detoxification and hormone balance. We describe the first case of hepatotoxicity due to Estrium Whey in a 51-year old female with metastatic breast cancer with clinical, laboratory and histopathological changes.

Ventricular assist device as a bridge to transplant, and extracorporeal membrane oxygenation for primary graft failure in a child with hemophilia A.

OBJECTIVE: To report the management of hemophilia in a patient with dilated cardiomyopathy during application of the Berlin-Heart biventricular assist. DESIGN: Case report. INTERVENTIONS AND RESULTS: The Berlin-Heart biventricular assist was successfully used as a bridge to heart transplantation (178 days) in a child with hemophilia A; post-transplant extracorporeal membrane oxygenation was implanted until the patient recovered from primary graft failure. Anticoagulant therapy varied as a function of patient status: 1) postoperative bleeding was treated by partial replacement of deficient factors (VII and VIII) and hemoderivatives in order to maintain factor VIII at 50% of normal levels; 2) once the bleeding had stopped, the effect of the hemophilia itself maintained the degree of anticoagulation required by the ventricular assist device; and 3) transplant surgery was followed by complete replacement of factor VIII and intravenous heparinization (a simple way of preventing clot formation in the device and to ensure proper scarring of surgical wounds). CONCLUSIONS: Selection of anticoagulant therapy as a function of patient status in terms of bleeding and surgical-wound scarring progress is vital for the proper functioning of support techniques (Berlin-Heart biventricular assist and extracorporeal membrane oxygenation) in hemophiliac patients. Collagen dressings placed on surgical wounds achieved good functional and aesthetic results, as well as mechanically isolating the scars from the exterior.

Feasibility, acceptability and impact of a telephone support service initiated in primary medical care to help Arabic smokers quit.

Evidence-based tobacco control in ethnic minorities is compromised by the near absence of rigorous testing of interventions in either prevention or cessation. This randomised controlled trial was designed to evaluate the feasibility, acceptability and impact of a culturally specific cessation intervention delivered in the context of primary medical care in the most culturally diverse region of New South Wales. Adult Arabic smokers were recruited from practices of 29 general practitioners (GPs) in south-west Sydney and randomly allocated to usual care (n=194) or referred to six sessions of smoking cessation telephone support delivered by bilingual psychologists (n=213). Although 62.2% of participants indicated that telephone support would benefit Arabic smokers, there were no significant differences at 6 or 12 months between intervention and control groups in point prevalence abstinence rates (11.7% vs 12.9%, P=0.83; 8.4% vs 11.3%, P=0.68, respectively) or the mean shift in stage-of-change towards intention to quit. As participants and GPs found telephone support acceptable, we also discuss redesign and the unfulfilled obligation to expand the evidence base in tobacco control from which the ethnic majority already benefits.

Smoking patterns and readiness to quit--a study of the Australian Arabic community.

BACKGROUND: Smoking cessation interventions have typically focused on majority populations who, in Australia, are English speaking. There has been an overall decline in the prevalence of smoking in the Australian community. However, there remains a relative paucity of useful information about tobacco use and the effectiveness of tobacco interventions among specific ethnic minorities. OBJECTIVE: To determine associations of tobacco use and tobacco control indicators for Arabic speakers seen in the Australian general practice setting. METHODS: A cross sectional study in a consecutive sample of Arabic patients (n=1371) attending the practices of 29 Arabic speaking general practitioners in Sydney, New South Wales. RESULTS: Twenty-nine (53.7%) of 54 eligible Arabic speaking GPs in southwest Sydney participated in this study. Of 1371 patients seen, 29.7% were smokers. Smokers were more likely to report poorer health (chi2=21.7, df=1, p<0.001); 35.7% reported high nicotine dependence. Dependence was more in men (chi2=11.7, df=1, p<001) and those who reported poorer health (chi2=4.9, df=1, p<0.03); 35.9% had attempted to quit in the previous year; 17% were in preparation stage of change; 42.7% recalled quit advice. Poorer self reported health status (AOR=2.13, 95% CI: 1.14-3.97, p=0.017) and unemployment (AOR=1.69, 95% CI: 1.51-4.90, p=0.033) were independent predictors of advice from a health professional, most often a GP (71%). CONCLUSION: Our study confirms previous reports that the proportion of self reported current smokers among the Arabic community is higher than for the Anglo-European majority. There is a need for ethno specific campaigns in tobacco control.

Recovery of the water content of hydrogel contact lenses after use.

The aim of the present study is to determine the amount and time of water-content recovery of ionic contact lenses (Surevue and Acuvue) and non-ionic contact lenses (Soflens 38, Soflens 66 and Optima 38) when submerged in maintenance solution for 9 h. Using a hand-held refractometer, we measured the water content of the lenses upon removal from the eye, and after 15, 30, 45 min, 1, 2 and 9 h in soaking solution. Both ionic lenses presented pre-wear water content of 58%, while two of the non-ionic ones (Soflens 38 and Optima 38) registered 38.6% and the third (Soflens 66) had 66%. We found a significant increase (p < 0.05) in water content after submersion in the Opti-Free solution for all the time periods studied and for all lenses. The water-content recovery was faster in the non-ionic lenses (Soflens 38, Soflens 66 and Optima 38) than in the ionic ones (Surevue and Acuvue), especially at 15 min. After 9 h in the soaking solution, the Surevue Acuvue, Soflens 38, Soflens 66 and Optima 38 lenses reached water-content recovery values of 99.7%, 99.6%, 99.8%, 99.8% and 99.6%, respectively, without significant differences (p > 0.05) between ionic and non-ionic lenses, although the non-ionic lenses reached slightly higher water-content values at all the times studied. These results indicate that when the refractometer technique is used to measure water content, the lens types used in this study regain their initial water-content values after 9 h in soaking solution. These data indicate the time needed for hydrogel contact lenses to reach their maximum water content after removal from the eye and submersion in maintenance solution. By ensuring maximum water content and minimum reduction of oxygen for the cornea, the specialist can help avoid clinical signs and symptoms related to low water content in the lenses.

Análisis de los tiempos de demora en un programa de consulta única / Analysis of delay times in a single-consultation program

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