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1.
J Microbiol Methods ; 107: 71-3, 2014 Dec.
Article in English | MEDLINE | ID: mdl-25193437

ABSTRACT

We performed the first evaluation study of the new HyBeacon based FluoroType(®) MRSA assay for the detection of MRSA directly from 617 patient specimens. Using culture as the reference method sensitivity and specificity were higher than 95%. Results were available within 2.5h, including DNA extraction.


Subject(s)
Methicillin-Resistant Staphylococcus aureus/genetics , Polymerase Chain Reaction/methods , Staphylococcal Infections/diagnosis , Staphylococcal Infections/microbiology , Humans , Methicillin-Resistant Staphylococcus aureus/classification , Reproducibility of Results , Sensitivity and Specificity
2.
Clin Lab ; 59(9-10): 1179-81, 2013.
Article in English | MEDLINE | ID: mdl-24273945

ABSTRACT

We performed the first evaluation study of the new FluoroType MTB assay, a HyBeacon based nucleic acid amplification test for the direct detection of Mycobacterium tuberculosis in respiratory tract specimens. Using mycobacterial culture as a reference, 661 specimens were tested. Sensitivity and specificity were 95.1% and 96.4% for all specimens, sensitivity was 100% for smear-positive and 84.6% for smear negative specimens, respectively. The FluoroType MTB assay showed fast and accurate results within 3 hours, including DNA extraction.


Subject(s)
Lung/microbiology , Mycobacterium tuberculosis/isolation & purification , Nucleic Acid Amplification Techniques , Respiratory System/microbiology , DNA, Bacterial/analysis , Humans , Mycobacterium tuberculosis/genetics , Reproducibility of Results , Sensitivity and Specificity
3.
Clin Lab ; 58(11-12): 1319-21, 2012.
Article in English | MEDLINE | ID: mdl-23289206

ABSTRACT

We evaluated the performance of a PCR-based dipstick assay used in our routine laboratory for the direct detection of Methicillin resistant Staphylococcus aureus (MRSA). 2941 clinical swab specimens were evaluated. Sensitivity and specificity were 94.1% and 98.3%, respectively. The PCR assay combines low instrumentation costs and minor hands-on-time with reliable results for MRSA identification.


Subject(s)
Methicillin-Resistant Staphylococcus aureus/isolation & purification , Polymerase Chain Reaction/methods , Humans , Retrospective Studies
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