ABSTRACT
La resonancia magnética nuclear (RMN) constituye una fuerte alternativa para estudios estructurales, evaluación de identidad y cuantificación de ingredientes farmacéuticos activos (IFA). En las dos últimas décadas la aplicación de la resonancia magnética nuclear cuantitativa (RMNc) tuvo un creciente impacto en la cuantificación de compuestos, fundamentalmente orgánicos. Varios resultados obtenidos mediante la RMNc han ido creando un lugar para la técnica en la industria biofarmacéutica. La RMNc incluye experimentos de RMN con algunos parámetros modificados, con el objetivo de obtener señales cuantificables. Basada en algunos de los reportes más relevantes, la presente revisión aborda algunas de las aplicaciones de la RMN para vacunas basadas en polisacáridos o glicoproteínas. El trabajo hace especial énfasis en detallar algunos aspectos que caracterizan la RMNc, así como sus aplicaciones(AU)
Nuclear Magnetic Resonance has become the choice for structural studies, identity assays and simultaneous quantification of active pharmaceutical ingredient of different polysaccharide-based vaccine. In the last two decades, the application of quantitative Nuclear Magnetic Resonance had an increasing impact to support several quantification necessities. The technique involves experiments with several modified parameters in order to obtain spectra with quantifiable signals. The present review is supported by some recent relevant reports and it discusses several applications of NMR in carbohydrate-based vaccines. Moreover, it emphasizes and describes several parameters and applications of quantitative Nuclear Magnetic Resonance(AU)
Subject(s)
Magnetic Resonance Spectroscopy/methods , Evaluation Studies as TopicABSTRACT
Neisseria meningitidis constitutes the main cause of meningococcal disease in infants. Serogroups A, B, C, W135, Y, and X have the higher incidence in young children and teenagers. The use of polyvalent conjugate carbohydrate-based vaccines has decreased the meningococcal infection around the world. Recently, the serogroup X has been found to be responsible of different outbreaks of meningococcal diseases, mainly in "Meningitis Belt" of Africa and the structure of the repetitive unit of the capsular polysaccharide has been confirmed through a monodimensional (13)C NMR study. No further characterization studies have been carried out, especially with the use of other nuclei. In this paper a novel method for quantification of the N. meningitidis serogroup X by proton qNMR is reported. Deep characterization of the serogroup X polysaccharide was also carried out by combination of correlation experiments involving (13)C, (1)H, and (31)P nuclei.
Subject(s)
Bacterial Capsules/chemistry , Neisseria meningitidis/chemistry , Nuclear Magnetic Resonance, Biomolecular/methods , Polysaccharides, Bacterial/chemistry , Bacterial Capsules/immunology , Carbohydrate Conformation , Carbon Isotopes , Linear Models , Molecular Structure , Neisseria meningitidis/immunology , Phosphorus Isotopes , Polysaccharides, Bacterial/immunology , Protons , Reproducibility of ResultsABSTRACT
A series of 3-[(2,5-dihydro-6-hydroxy-2-methyl)-5-oxo-cis-triazin-3-yl]-thiomethyl-cefalosporins with various 3-phenyl-2-propenoyl substituted groups at the 7beta-position were synthesized, structurally characterized and evaluated for antibacterial activity in vitro. To prepare these derivatives by the Vilsmeier's reagent method, it was necessary to carefully control the reaction conditions in order to avoid the formation of the biologically inactive alpha epimer. The NMR studies showed that the 3-phenyl-2-propenoyl moiety has little effect on chemical shifts of cephem nucleus protons and carbon atoms. Some of these cephalosporin derivatives showed good in vitro activity against methicillin sensible strains of Staphylococcus aureus (MSSA) and coagulase negative Staphylococcus (MSCoNS). Particularly effective were the compounds carrying a 3-(2'-chlorophenyl)-2-propenoyl or 2-methyl-3-phenyl-2-propenoyl moiety at 7beta-position, both with an antibacterial potency close to cefazoline and higher than cefuroxime. All the synthesized cephalosporins were inactive against methicillin resistant strains of Staphylococcus aureus (MRSA) and coagulase negative Staphylococcus (MRCoNS).
Subject(s)
Anti-Bacterial Agents/chemical synthesis , Anti-Bacterial Agents/pharmacology , Cephalosporins/chemical synthesis , Cephalosporins/pharmacology , Anti-Bacterial Agents/chemistry , Bacteria/drug effects , Cephalosporins/chemistry , Magnetic Resonance Spectroscopy , Microbial Sensitivity TestsABSTRACT
A selective and rapid capillary zone electrophoresis method for determination of the multicomponent aminoglycoside antibiotic gentamicin is described. Baseline separation of gentamicin C1, C1a, C2, C2a and C2b components was achieved with a background electrolyte containing 0.35 mM cetyl trimethylammonium bromide, 3% methanol and 90 mM sodium pyrophosphate (pH 7.4) and detected directly with UV detection without derivatization. Quantitative analysis was performed and illustrated the potential use of capillary electrophoresis for the identification and quantitation of gentamicin components, but the application of this method is limited to a gentamicin concentration range of 2-6 mg/ml.
Subject(s)
Anti-Bacterial Agents/analysis , Electrophoresis, Capillary/methods , Gentamicins/analysis , Gentamicins/chemistry , Spectrophotometry, Ultraviolet/methods , Anti-Bacterial Agents/chemistry , Cetrimonium , Cetrimonium Compounds/chemistry , Diphosphates/chemistry , Electrolytes/chemistry , Hydrogen-Ion Concentration , Methanol/chemistry , Molecular Structure , Sensitivity and Specificity , Time FactorsABSTRACT
(1)H and (13)C NMR spectroscopic data for 5alpha-androstanes and halo-5alpha-androstanes with different substituents at positions C-3, C-9, C-11 and C-17 were examined and assigned by a combination of 1D and 2D NMR experiments. The substituent effects on the (13)C chemical shifts were compared with those of epi-androsterone, used as a reference compound. The coupling constants (n)J((19)F,(13)C) were measured for compounds 6, 8, 11 and 14.
Subject(s)
Androstanes/chemistry , Magnetic Resonance Spectroscopy/methods , Magnetic Resonance Spectroscopy/standards , Molecular Conformation , Reference ValuesABSTRACT
The (1)H and (13)C spectroscopic data for 7beta-(cinnamoyl-substituted)amino-3-acetoxymethyl-cephalosporins were fully assigned by a combination of one- and two-dimensional experiments. Substitution on the aromatic ring and on the double-bond alpha-position of the cinnamoyl moiety has little influence on the spectroscopic properties of the 7beta-aminocephalosporanic acid parent moiety.
Subject(s)
Carbon Isotopes , Cephalosporins/chemistry , Cephalosporins/standards , Magnetic Resonance Spectroscopy/methods , Magnetic Resonance Spectroscopy/standards , Protons , Reference Values , Cephalosporins/analysis , Cephalosporins/classification , ItalyABSTRACT
Twenty 3-acetoxymethyl cephalosporin derivatives, with various cinnamoyl (3-phenyl-2-propenoyl) substituted groups at the 7beta-position, were synthesized and evaluated for antibacterial activity in vitro. Some of these cephalosporin derivatives showed good selective activity against Gram-positive bacteria. Although substitution on the aromatic ring of cinnamoyl moiety generally reduced antimicrobial activity against Staphylococcus sp. and Enterococcus sp., a hydroxy group at the para position, and particularly ortho, para di-chloro substitution, improved the activity against methicillin resistant strains of Staphylococcus aureus (MRSA). Substitution on the double bond alpha position of the cinnamoyl moiety also affected the antimicrobial activity. A cyano group attached to this position increased activity against both negative coagulase Staphylococcus and Enterococcus sp. and extended the antibacterial spectrum towards Gram-negative bacteria.
Subject(s)
Anti-Bacterial Agents/chemistry , Cephalosporins/chemistry , Cinnamates/chemistry , Anti-Bacterial Agents/pharmacology , Cephalosporins/pharmacology , Cinnamates/pharmacology , Humans , Microbial Sensitivity Tests , Staphylococcus aureus/drug effects , Staphylococcus aureus/physiology , Structure-Activity RelationshipABSTRACT
Cefpodoxime proxetil, a third-generation cephalosporin for oral administration, was synthesized by a method based on the following sequence of reactions: acylation of 7-aminocephalosporanic acid (7-ACA) with S-benzothiazol-2-yl(2-amino-4-thiazolyl)(methoxyimino)thioacetate (MAEM), chloroacetylation of the cefotaxime formed with chloroacetyl chloride, esterification of the acid function with 1-iodoethyl isopropyl carbonate and final cleavage of chloroacetamide protective group by treatment with thiourea in N,N-dimethylacetamide. The developed procedure allows us to obtain better yields of cefpodoxime proxetil and to eliminate the final purification step by column chromatography, necessary during the synthesis of this antibiotic by the previously reported methods.
Subject(s)
Acetates/chemical synthesis , Anti-Bacterial Agents/chemical synthesis , Ceftizoxime/analogs & derivatives , Ceftizoxime/chemical synthesis , Thiazoles/chemical synthesis , Acylation , Administration, Oral , Benzothiazoles , Cephalosporins/chemistry , Chemistry, Pharmaceutical , Drug Compounding , Cefpodoxime ProxetilABSTRACT
Cefdinir, a broad spectrum third-generation cephalosporin for oral administration, was prepared by the following synthetic pathway: synthesis of diphenylmethyl 7beta-amino-3-vinyl-3-cephem-4-carboxylate hydrochloride from 7-aminocephalosporanic acid (7-ACA), preparation of sodium 2-(2-tritylaminothiazol-4-yl)-(Z)-2-(tritylhydroxyimino) acetate from ethyl acetoacetate, coupling of both intermediaries to obtain diphenylmethyl 7beta-[2-(2-tritylaminothiazol-4-yl)-(Z)-2-tritylhydroxyimino-3-vinyl-3-cephem-4-carboxylate and final cleavage of trityl and diphenylmethyl protective groups. This procedure allows to obtain better yields of cefdinir and to avoid the use of diketene during the synthesis of this antibiotic by the previously reported method.
