ABSTRACT
The effect of adding hydrolysable tannins to the diet of fattening boars was studied. Performance, reproductive organ weights, salivary gland morphology, boar taint compounds and skatole metabolism were evaluated. At 123 days of age and 52 ± 6 kg liveweight, 24 Landrace × Large White boars were assigned within a litter to four treatment groups: control (T0 fed mixture with 13.2 MJ/kg, 17.5% crude proteins) and three experimental diets for which the T0 diet was supplemented with 1%, 2% and 3% of hydrolysable tannin-rich extract (T1, T2 and T3, respectively). Pigs were kept individually with ad libitum access to feed and water and slaughtered at 193 days of age and 122 ± 10 kg liveweight. Adding hydrolysable tannins to the diet had no negative effect on growth performance at 1% and 2%, whereas the 3% inclusion reduced feed intake and resulted in an adaptive response of the salivary glands (particularly parotid gland hypertrophy). Relative to T0, fat tissue skatole concentration was increased in the T1 group, but was similar in T2 and T3. Across treatments tissue skatole concentrations were proportional to the activity of hepatic CYP450. The results indicate the potential of tannin supplementation to reduce boar taint although further investigations are needed in order to establishing optimal dosage.
Subject(s)
Hydrolyzable Tannins/pharmacology , Intestinal Mucosa/metabolism , Liver/metabolism , Skatole/metabolism , Swine/metabolism , Animal Feed/analysis , Animals , Cytochrome P-450 Enzyme System/metabolism , Diet/veterinary , Gastrointestinal Contents/chemistry , Gene Expression Regulation, Enzymologic/drug effects , Genitalia, Male/metabolism , Hydrolyzable Tannins/chemistry , Male , Salivary Glands/metabolism , Tissue DistributionABSTRACT
Monitoring of plant protection product (PPP) residues in "Golden Delicious" apples was performed in 2011-2013, where 216 active substances were analysed with three analytical methods. Integrated pest management (IPM) production and improved IPM production were compared. Results were in favour of improved IPM production. Some active compounds determined in IPM production (boscalid, pyraclostrobin, thiacloprid and thiametoxam) were not found in improved IPM production. Besides that, in 2011 and 2012, captan residues were lower in improved IPM production. Risk assessment was also performed. Chronic exposure of consumers was low in general, but showed no major differences for IPM and improved IPM production for active substances determined in both types of production. Analytical results were compared with the European Union report of 2010 where 1.3% of apple samples exceeded maximum residue levels (MRLs), while MRL exceedances were not observed in this survey.
Subject(s)
Crops, Agricultural/chemistry , Food Contamination/prevention & control , Fruit/chemistry , Malus/chemistry , Pest Control/methods , Pesticide Residues/analysis , Acaricides/analysis , Acaricides/toxicity , Biphenyl Compounds/analysis , Biphenyl Compounds/toxicity , Carbamates/analysis , Carbamates/toxicity , Crops, Agricultural/microbiology , Crops, Agricultural/parasitology , Crops, Agricultural/standards , Crosses, Genetic , European Union , Food Quality , Fruit/microbiology , Fruit/parasitology , Fruit/standards , Fungicides, Industrial/analysis , Fungicides, Industrial/toxicity , Guidelines as Topic , Humans , Insecticides/analysis , Insecticides/toxicity , Malus/microbiology , Malus/parasitology , Neonicotinoids , Niacinamide/analogs & derivatives , Niacinamide/analysis , Niacinamide/toxicity , Nitro Compounds/analysis , Nitro Compounds/toxicity , Oxazines/analysis , Oxazines/toxicity , Pesticide Residues/standards , Pesticide Residues/toxicity , Pyrazoles/analysis , Pyrazoles/toxicity , Pyridines/analysis , Pyridines/toxicity , Risk Assessment , Slovenia , Strobilurins , Thiamethoxam , Thiazines/analysis , Thiazines/toxicity , Thiazoles/analysis , Thiazoles/toxicityABSTRACT
Using a soil column experiment, we compared the effect of a single dose and weekly additions of ethylenediaminetetraacetic acid (EDTA) and ethylenediaminedissuccinate (EDDS) on the uptake of Pb, Zn, and Cd by Chinese cabbage [Brassica rapa L. subsp. pekinensis (Lour.) Hanelt], and on the leaching of heavy metals through the soil profile. The analysis of plant material revealed that both chelates increased the concentrations of Pb and, to a lesser extent, also of Zn and Cd in the leaves of the test plant. The most effective applications were single doses of 10 mmol EDTA and EDDS kg(-1) soil, which caused the concentrations of Pb in the shoots to increase 94.2- and 102.3-fold, respectively, relative to the control. The same dose of EDTA increased the concentration of Zn and Cd in the leaves 4.3- and 3.8-fold and of EDDS 4.7- and 3.5-fold, respectively. In treatments with weekly additions and lower concentrations of both chelates, EDTA was more effective than EDDS in increasing the plant uptake of Pb. In soil columns treated with weekly additions of 10 mmol kg(-1) EDTA, on average 22.7, 7.0, and 39.8% of initial total Pb, Zn, and Cd in the soil were leached through the soil profile. The same amount of EDDS caused much lower leaching of Pb and Cd--only 0.8 and 1.5% of initial total concentrations. Leaching of Zn, 6.2% of the total concentration, was comparable with the EDTA treatment. A biotest with red clover (Trifolium pratense L.) indicated a greater phytotoxic effect of EDTA than EDDS addition. EDDS was also less toxic to soil fungi, as determined by phospholipid fatty acid (PLFA) analysis, and caused less stress to soil microorganisms, as indicated by the trans to cis PLFA ratio. Chelate addition did not prevent the development of arbuscular mycorrhiza on red clover.
