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1.
J Clin Psychol Med Settings ; 31(2): 493-500, 2024 Jun.
Article in English | MEDLINE | ID: mdl-38265700

ABSTRACT

Motivational interviewing (MI)-based interventions can be effective for people living with HIV (PLWHIV) with medical follow-up problems. This study aimed to assess the stage of motivation to attend medical appointments of PLWHIV with medical follow-up problems and to evaluate a single telephone session MI intervention directed to engage them in care. The change in stage was evaluated before and after the intervention, and attending medical appointments was monitored for six months. Thirty-nine PLWHIV participated; 51.3% were in precontemplation/contemplation and 49.7% in preparation/action stage. Most (excluding those in the action stage from the beginning) (n = 22, 71.0%) advanced to another stage after the intervention (z = - 4.235, p < .001), and most did not miss the following appointments. Brief and remote MI interventions could be useful in low-resource settings, especially for those in the early stages of change, to explore motivations related with missing medical appointments.


Subject(s)
HIV Infections , Motivational Interviewing , Telephone , Humans , Motivational Interviewing/methods , Male , Female , HIV Infections/psychology , Adult , Middle Aged , Motivation , Follow-Up Studies
2.
Data Brief ; 47: 109007, 2023 Apr.
Article in English | MEDLINE | ID: mdl-36909016

ABSTRACT

This article presents the outdoor and synthetic performance data concerning the main electrical parameters estimated from the I-V curve for three photovoltaic technologies (HIT, m-Si and CIGS) and the weather conditions (irradiance, ambient and panel temperature). Synthetic data were generated by simulating in OpenModelica software the impact of weather conditions on device performance, considering an irradiance range between 50 and 1300 W/m2. The outdoor data corresponds to the performance of the evaluated PV modules in outdoor tests in Medellin-Colombia for ten months using capacitive I-V tracers. In both cases, different capacitor values were considered to evaluate the effect on the I-V curve behavior of devices.

3.
Clin. transl. oncol. (Print) ; 24(5): 829-835, mayo 2022. ilus, graf
Article in English | IBECS | ID: ibc-203798

ABSTRACT

PurposeTo present the first results of intraoperative irradiation (IORT) in breast cancer with a low-energy photon system used as partial breast irradiation (PBI) or as an anticipated boost before whole breast hypo-fractionated irradiation (IORT + WBI), concerning tolerance, side effects, quality of life, and patient-reported outcomes.Materials and methodsEighty patients treated with an Intrabeam® system of 50 kV X-rays received a 20 Gy dose intraoperatively were included. Moderate daily hypofractionation of 2.7 Gy in 15 fractions up to 40.5 Gy was administered if high-risk factors were present. Acute post-operative toxicity, surgery complications, chronic toxicity, patient-reported cosmesis and Breast-Q questionnaire were performed at follow-up visits.ResultsThirty-one patients were treated as PBI and the remaining 49 as IORT + WBI. Only the IORT + WBI group presented acute toxicity, mainly mild acute dermatitis (11 patients) and one subacute mastitis. A total of 20 patients presented fibrosis (18 patients grade I, 2 patients grade II), 15 (30.5%) patients in the IORT + WBI group and 3 (9.6%) patients in the group of PBI. The cosmesis evaluation in 73 patients resulted poor, fair, good or excellent in 2, 7, 38 and 26 patients, respectively. In PBI group Breast-Q scored higher, especially in terms of their psychosocial well-being (78 vs 65) and satisfaction with radiation-induced toxicity (77 vs 72, respectively) compared to IORT + WBI group.ConclusionIORT is a well-tolerated procedure with low toxicity, good cosmesis and favorable patient-reported outcomes mainly when administered as PBI.


Subject(s)
Humans , Female , Unilateral Breast Neoplasms/etiology , Unilateral Breast Neoplasms/radiotherapy , Unilateral Breast Neoplasms/surgery , Mastectomy, Segmental , Radiation Injuries/etiology , Radiotherapy, Adjuvant/methods , Quality of Life , Radiation Dose Hypofractionation
4.
Clin Transl Oncol ; 24(5): 829-835, 2022 May.
Article in English | MEDLINE | ID: mdl-34792725

ABSTRACT

PURPOSE: To present the first results of intraoperative irradiation (IORT) in breast cancer with a low-energy photon system used as partial breast irradiation (PBI) or as an anticipated boost before whole breast hypo-fractionated irradiation (IORT + WBI), concerning tolerance, side effects, quality of life, and patient-reported outcomes. MATERIALS AND METHODS: Eighty patients treated with an Intrabeam® system of 50 kV X-rays received a 20 Gy dose intraoperatively were included. Moderate daily hypofractionation of 2.7 Gy in 15 fractions up to 40.5 Gy was administered if high-risk factors were present. Acute post-operative toxicity, surgery complications, chronic toxicity, patient-reported cosmesis and Breast-Q questionnaire were performed at follow-up visits. RESULTS: Thirty-one patients were treated as PBI and the remaining 49 as IORT + WBI. Only the IORT + WBI group presented acute toxicity, mainly mild acute dermatitis (11 patients) and one subacute mastitis. A total of 20 patients presented fibrosis (18 patients grade I, 2 patients grade II), 15 (30.5%) patients in the IORT + WBI group and 3 (9.6%) patients in the group of PBI. The cosmesis evaluation in 73 patients resulted poor, fair, good or excellent in 2, 7, 38 and 26 patients, respectively. In PBI group Breast-Q scored higher, especially in terms of their psychosocial well-being (78 vs 65) and satisfaction with radiation-induced toxicity (77 vs 72, respectively) compared to IORT + WBI group. CONCLUSION: IORT is a well-tolerated procedure with low toxicity, good cosmesis and favorable patient-reported outcomes mainly when administered as PBI.


Subject(s)
Breast Neoplasms , Radiation Injuries , Breast , Breast Neoplasms/etiology , Breast Neoplasms/radiotherapy , Breast Neoplasms/surgery , Female , Humans , Mastectomy, Segmental , Quality of Life , Radiation Dose Hypofractionation , Radiation Injuries/etiology , Radiotherapy, Adjuvant/methods
5.
Clin. transl. oncol. (Print) ; 19(7): 853-857, jul. 2017. tab, graf
Article in English | IBECS | ID: ibc-163439

ABSTRACT

Introduction. Neoadjuvant radiochemotherapy followed by radical surgery is the standard approach in advanced rectal carcinoma. Tumor response is determined in histological specimen. Objective. To assess predictive factors for survival in 115 patients. Patients and Method. 115 patients treated with neoadjuvant radiochemotherapy followed by radical surgery with total mesorectal excision, in our hospital from January 2007 to December 2014. All patients received pelvic radiotherapy with concomitant chemotherapy, followed by radical surgery and in some adjuvant chemotherapy. Results. In univariate analysis, distance to anal verge, radial margin, perineural invasion, and good grade regression are predictive factors for both, specific and disease free survival; and in multivariant, only radial margin and perineural invasion were predictive factors for survival. We found distance to anal verge (<5 cm) as the only clinical factor to predict a positive margin in the histologic specimen. Conclusions. Perineural invasion and positive radial margin are predictive factors for both specific and disease free survival (AU)


No disponible


Subject(s)
Humans , Male , Female , Adult , Middle Aged , Aged , Aged, 80 and over , Rectal Neoplasms/drug therapy , Rectal Neoplasms/radiotherapy , Neoadjuvant Therapy/methods , Chemoradiotherapy/methods , Predictive Value of Tests , Rectal Neoplasms/surgery , Rectal Neoplasms , Retrospective Studies , Kaplan-Meier Estimate , Multivariate Analysis
6.
Clin Transl Oncol ; 19(7): 853-857, 2017 Jul.
Article in English | MEDLINE | ID: mdl-28120325

ABSTRACT

INTRODUCTION: Neoadjuvant radiochemotherapy followed by radical surgery is the standard approach in advanced rectal carcinoma. Tumor response is determined in histological specimen. OBJECTIVE: To assess predictive factors for survival in 115 patients. PATIENTS AND METHOD: 115 patients treated with neoadjuvant radiochemotherapy followed by radical surgery with total mesorectal excision, in our hospital from January 2007 to December 2014. All patients received pelvic radiotherapy with concomitant chemotherapy, followed by radical surgery and in some adjuvant chemotherapy. RESULTS: In univariate analysis, distance to anal verge, radial margin, perineural invasion, and good grade regression are predictive factors for both, specific and disease free survival; and in multivariant, only radial margin and perineural invasion were predictive factors for survival. We found distance to anal verge (<5 cm) as the only clinical factor to predict a positive margin in the histologic specimen. CONCLUSIONS: Perineural invasion and positive radial margin are predictive factors for both specific and disease free survival.


Subject(s)
Adenocarcinoma/mortality , Chemoradiotherapy, Adjuvant/mortality , Neoadjuvant Therapy/mortality , Rectal Neoplasms/mortality , Adenocarcinoma/pathology , Adenocarcinoma/therapy , Adult , Aged , Aged, 80 and over , Female , Follow-Up Studies , Humans , Male , Middle Aged , Prognosis , Rectal Neoplasms/pathology , Rectal Neoplasms/therapy , Retrospective Studies , Survival Rate
7.
Clin. transl. oncol. (Print) ; 13(7): 504-508, jul. 2011. tab, ilus
Article in English | IBECS | ID: ibc-124694

ABSTRACT

AIMS AND BACKGROUND: To evaluate the predictive factors of recurrence in cervical cancer treated with radical radiochemotherapy. METHODS: A retrospective analysis of 56 women was performed. Response was assessed using the RECIST response. Overall survival and disease-free survival curves were estimated by the Kaplan-Meier method and the Cox proportional hazards model was used to analyse predictors of recurrence. RESULTS: Local recurrence was documented in 16 patients and distant metastases in 15. The Kaplan-Meier survival probabilities were 95.1 ± 6.4% at 3 years and 80.4 ± 13.1% at 5 years and the Kaplan-Meier curve values for disease-free survival were 60.3 ± 14.3% at 3 years and 53.0 ± 15.7% at 5 years. Thirty-five patients were alive and 21 patients died, 19 from metastatic disease and 2 from other causes. Complete response after chemoradiation therapy, squamous cell carcinoma and tumour size ≤ 4 cm were significantly associated with outcome. In the Cox regression model, tumour size > 4 cm (hazard ratio 7.48; 95% CI 2.71-20.6; p < 0.001) and partial response (hazard ratio 7.09; 95% CI 2.82-17.8; p < 0.001) were predictive factors for disease-free survival and partial response (hazard ratio 3.7; 95% CI 1.3-10.1; p < 0.001) and non-squamous cell carcinoma (hazard ratio 3.5; 95% CI 1.2-9.7; p < 0.001) were predictive factors for overall survival. CONCLUSIONS: Non-squamous histology and partial response were independent prognostic factors for overall survival and tumour size and partial response were independent prognostic variables for 5-year disease survival (AU)


Subject(s)
Humans , Male , Female , Adult , Middle Aged , Aged , Aged, 80 and over , Adenocarcinoma/therapy , Carcinoma, Squamous Cell/therapy , Neoplasm Recurrence, Local/pathology , Neoplasm Recurrence, Local/therapy , Adenocarcinoma/secondary , Brachytherapy/methods , Carcinoma, Squamous Cell/secondary , Combined Modality Therapy/methods , Combined Modality Therapy , Follow-Up Studies , Retrospective Studies , Time Factors , Survival Rate , Prognosis
8.
AIDS Care ; 23(3): 274-80, 2011 Mar.
Article in English | MEDLINE | ID: mdl-21347890

ABSTRACT

We assess the coverage of a Prevention of Mother-to-child Transmission (PMTCT) programme in Busia (Kenya) from 1 January 2006 to 31 December 2008 and estimate the risk of transmission of HIV. We also estimate the odds of HIV transmission according to pharmacological intervention received. Programme coverage was estimated as the proportion of mother-baby pairs receiving any antiretroviral (ARV) regimen among all HIV-positive women attending services. We estimated the mother-to-child transmission (MTCT) rate and their 95% confidence interval (95%CI) using the direct method of calculation (intermediate estimate). A case-control study was established among all children born to HIV-positive mothers with information on outcome (HIV status of the babies) and exposure (data on pharmacological intervention). Cases were all HIV-positive children and controls were the HIV-negative ones. Exposure was defined as: (1) complete protocol: ARV prescribed according World Health Organisation recommendations; (2) partial protocol: does not meet criteria for complete protocol; and (3) no intervention: ARVs were not prescribed to both mother and child. Babies were tested using DNA Polymerase Chain Reaction at six weeks of life and six weeks after breastfeeding ceased. In the study period, 22,566 women accepted testing, 1668 were HIV positive (7.4%; 95%CI 7.05-7.73); 1036 (62%) registered in the programme and 632 were lost. Programme coverage was 40.4% (95%CI 37.9-42.7). Out of the 767 newborns, 28 (3.6%) died, 148 (19.3%) defaulted, 282 (36.7%) were administratively censored and 309 (40.2%) babies completed the follow-up as per protocol; 49 were HIV positive and MTCT risk was 15.86% (95%CI 11.6-20.1). The odds of having an HIV-positive baby was 4.6 times higher among pairs receiving a partial protocol compared to those receiving a complete protocol and 43 times higher among those receiving no intervention. Our data show a good level of enrolment but low global coverage rate. It demonstrates that ARV regimens can be implemented in low resource rural settings with marked decreases of MTCT. Increasing the coverage of PMTCT programmes remains the main challenge.


Subject(s)
Anti-HIV Agents/therapeutic use , HIV Infections/transmission , Infectious Disease Transmission, Vertical/prevention & control , Pregnancy Complications, Infectious/drug therapy , Program Evaluation/standards , Adolescent , Adult , Antiretroviral Therapy, Highly Active , Epidemiologic Methods , Female , HIV Infections/epidemiology , Humans , Infant , Infant, Newborn , Kenya/epidemiology , Nevirapine/therapeutic use , Pregnancy , Rural Health , Treatment Outcome , Young Adult , Zidovudine/therapeutic use
9.
Hum Reprod ; 25(9): 2316-27, 2010 Sep.
Article in English | MEDLINE | ID: mdl-20634189

ABSTRACT

BACKGROUND: Sister chromatid cohesion is essential for ordered chromosome segregation at mitosis and meiosis. This is carried out by cohesin complexes, comprising four proteins, which seem to form a ring-like complex. Data from animal models suggest that loss of sister chromatid cohesion may be involved in age-related non-disjunction in human oocytes. Here, we describe the distribution of cohesins throughout meiosis in human oocytes. METHODS: We used immunofluorescence in human oocytes at different meiotic stages to detect cohesin subunits REC8, STAG3, SMC1 beta and SMC3, [also synaptonemal complex (SC) protein 3 and shugoshin 1]. Samples from euploid fetuses and adult women were collected, and 51 metaphase I (MI) and 113 metaphase II (MII) oocytes analyzed. SMC1 beta transcript levels were quantified in 85 maturing germinal vesicle (GV) oocytes from 34 women aged 19-43 years by real-time PCR. RESULTS: At prophase I, cohesin subunits REC8, STAG3, SMC1 beta and SMC3 overlapped with the lateral element of the SC. Short cohesin fibers are observed in the oocyte nucleus during dictyate arrest. All four subunits are observed at centromeres and along chromosomal arms, except at chiasmata, at MI and are present at centromeric domains from anaphase I to MII. SMC1 beta transcripts were detected (with high inter-sample variability) in GV oocytes but no correlation between SMC1 beta mRNA levels and age was found. CONCLUSIONS: The dynamics of cohesins REC8, STAG3, SMC1 beta and SMC3 suggest their participation in sister chromatid cohesion throughout the whole meiotic process in human oocytes. Our data do not support the view that decreased levels of SMC1 beta gene expression in older women are involved in age-related non-disjunction.


Subject(s)
Cell Cycle Proteins/metabolism , Chondroitin Sulfate Proteoglycans/metabolism , Chromosomal Proteins, Non-Histone/metabolism , Chromosome Aberrations , Meiosis , Nuclear Proteins/metabolism , Oocytes , Adult , Aging , Cell Cycle Proteins/genetics , Cell Nucleus/metabolism , Centromere/metabolism , Chromatids/genetics , Chromosomal Proteins, Non-Histone/genetics , DNA-Binding Proteins , Female , Fetus , Humans , Oocytes/chemistry , Oocytes/metabolism , RNA, Messenger/metabolism , Synaptonemal Complex/metabolism , Young Adult , Cohesins
10.
Hum Reprod ; 25(7): 1824-35, 2010 Jul.
Article in English | MEDLINE | ID: mdl-20488804

ABSTRACT

BACKGROUND: Comparative genomic hybridization (CGH) is a valuable alternative to fluorescence in situ hybridization (FISH) for preimplantation genetic screening (PGS) because it allows full karyotype analysis. However, this approach requires the cryopreservation of biopsied embryos until results are available. The aim of this study is to reduce the hybridization period of CGH, in order to make this short-CGH technique suitable for PGS of Day-3 embryos, avoiding the cryopreservation step. METHODS: Thirty-two fibroblasts from six aneuploid cell lines (Coriell) and 48 blastomeres from 10 Day-4 embryos, discarded after PGS by FISH with 9 probes (9-chr-FISH), were analysed by short-CGH. A reanalysis by the standard 72 h-CGH and FISH using telomeric probes was performed when no concordant results between short-CGH and FISH diagnosis were observed. The short-CGH was subsequently applied in a clinical case of advanced maternal age. RESULTS: In 100% of the fibroblasts analysed, the characteristic aneuploidies of each cell line were detected by short-CGH. The results of the 48 blastomeres screened by short-CGH were supported by both 72 h-CGH results and FISH reanalysis. The chromosomes most frequently involved in aneuploidy were 22 and 16, but aneuploidies for the other chromosomes, excepting 1, 10 and 13, were also detected. Forty-one of the 94 aneuploid events observed (43.6%) corresponded to chromosomes which are not analysed by 9-chr-FISH. CONCLUSIONS: We have performed a preliminary validation of the short-CGH technique, including one clinical case, suggesting this approach may be applied to Day-3 aneuploidy analysis, thereby avoiding embryo cryopreservation and perhaps helping to improve implantation rate after PGS.


Subject(s)
Aneuploidy , Blastomeres/ultrastructure , Comparative Genomic Hybridization/methods , Preimplantation Diagnosis/methods , Adult , Cell Line , Cryopreservation , Fibroblasts/ultrastructure , Humans , In Situ Hybridization, Fluorescence , Karyotyping , Maternal Age , Middle Aged
11.
Rev. senol. patol. mamar. (Ed. impr.) ; 20(3): 120-124, 2007. ilus, tab
Article in Spanish | IBECS | ID: ibc-74284

ABSTRACT

Introducción: Los cambios en el diagnóstico precoz, la cirugía,el tratamiento sistémico y los avances tecnológicos de laradioterapia han provocado un cambio, cualitativo y cuantitativo,en la utilización de las radiaciones en el tratamiento de laneoplasia de mama.Objetivo: Analizar los cambios en las indicaciones de irradiaciónexterna por neoplasia de mama desde el año 1990hasta la actualidad.Resultados: De enero 1990 a diciembre 2005 se realizaron4.545 irradiaciones por cáncer de mama, que representanel 29% del total. En el año 1990, el 43% de las indicacioneseran radicales y el 56% paliativas y actualmente el 82%son radicales y el 18% paliativas. También se constata un aumentodel tratamiento conservador, del 14 al 61%. Por elcontrario el resto de indicaciones, tras mastectomía, paliativosistémico o local disminuyen.Conclusiones: Actualmente el 29% de los tratamientos deun servicio de radioterapia son por cáncer de mama. Se detectaun aumento de la incidencia de la irradiación tras tratamientoconservador. Por el contrario, destaca una disminución dela irradiación tras mastectomía y de los tratamientos paliativos,tanto generales como locales, probablemente debido al aumentode la utilización de los tratamientos sistémicos(AU)


Introduction: Changes in early diagnosis, surgery, sistemictreatment and technological radiotherapy developments havemade a qualitative and quantitative changes, in the use of radiationin the treatment of breast cancer.Objective: Analyze the changes in the indication of radiationtherapy for breast cancer, since 1990 to nowadays.Results: From january 1990 to december 2005 we underwent4.545 irradiations for breast cancer (29% of total irradiations).In 1990, 43% of indications were radicals and 56% werepalliatives. Nowadays 82% are radicals and 18% are palliatives.Also, we can see an increase of the conservative treatment,from 14 to 61%. On the contrary, the rest of indications dicrease:after mastectomy, sistemic, palliative or local palliative.Conclusion: An increase in the incidence of breast canceris observed at the present time correspondig to 29% amountof treatments in a radiation in department. Also an increase ofincidence of the radiation after conservative treatment is confirmed.On the contrary, the incidence of the radiation aftermastectomy and palliative treatments decreases, probably dueto the use of the sistemic treatments(AU)


Subject(s)
Humans , Female , Adult , Middle Aged , Breast Neoplasms/radiotherapy , Radiotherapy/methods , Radiotherapy/statistics & numerical data , Mastectomy/trends , Mastectomy , Breast Neoplasms/epidemiology , Carcinoma, Intraductal, Noninfiltrating/radiotherapy , Indicators of Morbidity and Mortality
12.
Todo hosp ; (232): 636-639, dic. 2006. ilus
Article in Es | IBECS | ID: ibc-052070

ABSTRACT

La complejidad de los circuitos de los servicios de radioterapia, donde intervienen distintos departamentos, el carácter secuencial del tratamento con un elevado número de pacientes en cada una de las diferentes fases del proceso, y la obligada optimización en el uso de los aceleradores debido a la creciente presión asistencias, requieren un control de calidad exhaustivo en la gestión interna de los pacientes. Los programas comerciales presentes en mercado acostumbran a ser cerrados, inaccesibles y no adaptables a la realidad de cada centro. En el Institut d´Oncologia Radioteràpica (IOR) hemos desarrollado un modelo de programa informático que permite garantizar un riguroso control sobre estos aspectos y elimina el uso de las engorrosas listas en papel


No disponible


Subject(s)
Humans , Radiotherapy/trends , Nuclear Medicine Department, Hospital/organization & administration , 34002 , Patient-Centered Care/organization & administration
13.
Todo hosp ; (220): 570-576, oct. 2005. tab
Article in Spanish | IBECS | ID: ibc-59733

ABSTRACT

La radioterapia es una especialidad médica de gran complejidad técnica y que conlleva un cierto grado de riesgo en su aplicación. Por este motivo es objeto de una extensa y restrictiva regulación legal. En el presente artículo se analizan las consecuencias que tienen sobre ella las distintas disposiciones legales, haciendo especial hincapié en el Real Decreto de los Criterios de Calidad en Radioterapia (AU)


Because of the great technical complexity and the degree of risk in its application, Radiotherapy is a unique medical speciality with extensive and restrictive legal regulations. This work analyses the consequences of the different legal provisions, with special emphasis on the Royal Decree establishing the Quality Criteria in Radiotherapy (AU)


Subject(s)
Radiotherapy/ethics , Radiation Oncology/legislation & jurisprudence , Equipment Safety/standards , Safety/legislation & jurisprudence , Quality Control , Radiosurgery/legislation & jurisprudence , Radiation, Ionizing , Total Quality Management/legislation & jurisprudence , Medical Oncology/legislation & jurisprudence , Oncology Service, Hospital/legislation & jurisprudence
14.
Mol Reprod Dev ; 68(4): 507-14, 2004 Aug.
Article in English | MEDLINE | ID: mdl-15236337

ABSTRACT

The aim of this study was evaluate cortical granule (CG) distribution during in vitro maturation (IVM) and fertilisation of prepubertal goat oocytes compared to CG distribution of ovulated and in vitro fertilised oocytes from adult goats. Oocytes from prepubertal goats were recovered from a slaughterhouse and were matured in M199 with hormones and serum for 27 hr. Ovulated oocytes were collected from gonadotrophin treated Murciana goats. Frozen-thawed spermatozoa were selected by centrifugation in percoll gradient and were capacitated in DMH with 20% steer serum for 1 hr. Ovulated and IVM-oocytes were inseminated in DMH medium with steer serum and calcium lactate for 20 hr. Oocytes and presumptive zygotes were stained with FITC-LCA (Lens culinaris agglutinin labelled with fluorescein isothiocyanate) and observed under a confocal laser scanning microscope. Ultrastructure morphology of oocytes and presumptive zygotes were analysed by transmission electron microscopy (TEM). Prepubertal goat oocytes at germinal vesicle stage show a homogeneous CG distribution in the cytoplasm. IVM-oocytes at Metaphase II (MII) and ovulated oocytes presented CGs located in the cortex with the formation of a monolayer beneath to the plasma membrane. At 20 hr postinsemination (hpi), zygotes from IVM-oocytes showed a complete CG exocytosis whereas zygotes from ovulated oocytes presented aggregates of CGs located at the cortical region. Images by TEM detected that CGs were more electrodense and compacts in oocytes from prepubertal than from adult goats.


Subject(s)
Cytoplasmic Granules/physiology , Fertilization/physiology , Oocytes/cytology , Oocytes/physiology , Sexual Maturation/physiology , Animals , Cytoplasmic Granules/ultrastructure , Female , Goats , In Vitro Techniques , Male , Meiosis , Microscopy, Confocal , Oocytes/ultrastructure , Ovulation , Zygote/cytology , Zygote/physiology
15.
Theriogenology ; 57(5): 1397-409, 2002 Mar 15.
Article in English | MEDLINE | ID: mdl-12054199

ABSTRACT

Brilliant cresyl blue stain allows us to determine the activity of glucose-6-phosphate dehydrogenase (G6PD), an enzyme synthesized in growing oocytes but with decreased activity in oocytes that have finished their growth phase. The objective of this study was to evaluate the utility of the brilliant cresyl blue (BCB) test as an indirect measure of oocyte growth, in order to select competent prepubertal goat oocytes for in vitro embryo production. Oocytes were exposed to BCB diluted in PBS and were classified according to their cytoplasm coloration: oocytes with a blue cytoplasm or grown oocytes (BCB+) and oocytes without a blue cytoplasm or growing oocytes (BCB-). After exposure to different BCB concentrations, we evaluated in vitro maturation (IVM), in vitro fertilization (IVF) and embryo development parameters. We defined matured oocytes as those oocytes that reached the metaphase II (MII) stage after being cultured for 27 h. Oocytes showing two pronuclei at 20 h post-insemination were classified as normally fertilized oocytes. We assessed embryo development 8 days post-insemination and recorded the percentage of total embryos, morale and blastocysts. The mean percentage of BCB+ oocytes was 29.4%. Mean diameter of BCB+ oocytes (136.6+/-6.3 microm) was higher (P < 0.001) than that of BCB- oocytes (125.5+/-10.2 microm). The percentage of BCB+ oocytes reaching the MII stage (81.4%) was higher (P < 0.05) than that of BCB- (52.5%) and control oocytes (72.4%). Normal fertilization rate of BCB+ oocytes was also higher (23.5%) than that of BCB- (8.2%; P < 0.0001) and control oocytes (11.9%; P < 0.05). The percentages of total embryos undergoing development to >8-cell and the morula plus blastocyst stages were higher (P < 0.05) in the group of BCB+ (41.3 and 12.0%, respectively) than in BCB- oocytes (21.3 and 3.6%, respectively). In conclusion, the BCB test is a useful way to select more competent prepubertal goat oocytes for in vitro embryo production.


Subject(s)
Coloring Agents , Goats , Oocytes , Oxazines , Sexual Maturation , Tissue and Organ Harvesting/veterinary , Animals , Blastocyst/physiology , Cells, Cultured , Culture Techniques , Cytoplasm , Embryonic and Fetal Development , Female , Fertilization in Vitro/veterinary , Glucosephosphate Dehydrogenase/analysis , Morula/physiology , Oocytes/enzymology , Oocytes/physiology , Oocytes/ultrastructure , Tissue and Organ Harvesting/methods
16.
Hum Reprod ; 14(1): 207-10, 1999 Jan.
Article in English | MEDLINE | ID: mdl-10374121

ABSTRACT

The aim of this study was to evaluate the effect of methyl-prednisolone on the pregnancy rate in mice. For this reason, zona pellucida-intact and zona pellucida-free embryos at the blastocyst stage were transferred to recipient mice at day 2.5 of pseudopregnancy. Embryo transfer was performed into non-immunodepressed and immunodepressed groups of recipient mice using 0.3 or 0.6 microgram/g of 6 beta-methylprednisolone. A higher implantation and developmental rate of zona-free embryos transferred to the immunodepressed group of recipients was observed after using the higher dose of methylprednisolone.


Subject(s)
Glucocorticoids/pharmacology , Methylprednisolone/pharmacology , Pregnancy Rate , Pregnancy, Animal/drug effects , Animals , Dose-Response Relationship, Drug , Embryo Implantation/drug effects , Embryo Transfer , Embryonic and Fetal Development/drug effects , Female , Fetal Resorption/epidemiology , Immune System/drug effects , Immunoglobulin G/metabolism , Incidence , Mice , Mice, Inbred Strains , Pregnancy
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