A clinical investigation to assess the regenerative potential of Biostite® (hydroxyapatite, type-I collagen and chondroitin-sulphate) with or without Paroguide® type-I collagen and chondroitin sulphate) membrane in the treatment of periodontal intrabony defects appraised with surgical re-entry and computer-assisted densitometric image analysis.

Background: The traditional treatment procedures which aimed at treating periodontal disease did not result in true periodontal regeneration. Unpredictability about osseous defect fill after periodontal flap surgery has stemmed in the research of a variety of regenerative materials. This randomized clinical trial was conducted to compare regenerative potential of Biostite® bone graft material with and without Paroguide® a bioresorbable membrane in the treatment of periodontal osseous defects. Materials and Methods: Twenty sites from a total of from ten patients were chosen for the study. Those sites were divided into experimental site A (Biostite®) and experimental site B (Biostite® with Paroguide®) at random. Plaque index and gingival index (GI) were the clinical parameters noted at baseline, 3rd, 6th and 9th months, whereas the probing pocket depth, clinical attachment level and gingival recession were noted at baseline, 6th and 9th months. Radiographic evaluation was made by using computer-assisted densitometric analysis. Intrasurgical measurements were done at baseline and 9 months. Statistical analysis was done using paired t-test and un-paired t-test. Results: Both experimental site A and B showed a significant reduction in plaque and GI. All clinical parameters as well as radiographic image analysis showed highly significant improvement from baseline to 9 months for both sites. Inter-group comparison does not show statistically significant improvement. Conclusion: The results of this study suggested that both Biostite® bone graft and Paroguide® membrane have promised encouraging results in the management of periodontal intrabony defects, however, the groups did differed to each other statistically.

Electro-surgical management of a traumatic fibroma with clinical assessment of re-epithelialization using methylene blue assay: A clinico-histopathological case report.

This article addresses the clinical presentation, diagnosis, histological features, and management of a case of fibroma. Fibroma is proliferative fibrotic lesions of the gingiva and oral mucosa, which may cause aesthetic and functional problems. Fibrous hyperplasia and fibroepithelial hyperplasia are histological alternates of these nonneoplastic lesions. The case demonstrated an overgrowth in the lower back tooth region. The growth was pale in color, sessile, painless, and firm in consistency. The lesion caused discomfort when chewing food, the lesion was excised using dental electrocautery and sent for histopathological assessment. This lesion was caused by trauma or persistent irritation, and histological examination indicates that the cells in the lesion are from the oral mucous membrane. This case demonstrates the need for proper diagnosis, the role of biopsy, and histologic evaluation in the management of fibrotic lesions.

Clinical and antioxidant efficacy of 4% mangosteen gel as a local drug delivery in the treatment of chronic periodontitis: A placebo-controlled, split-mouth trial.

BACKGROUND: Currently, the knowledge on the clinical effect of 4% mangosteen gel as a local drug delivery, adjunctive to non-surgical periodontal therapy, on the gingival crevicular fluid (GCF) total antioxidant capacity (TAOC) levels in chronic periodontitis patients is limited. OBJECTIVES: The aim of the study was to evaluate and compare the efficacy of 4% mangosteen gel and a placebo gel as an adjunct to scaling and root planing (SRP) on clinical and biochemical parameters in chronic periodontitis patients. MATERIAL AND METHODS: A total of 50 test sites from 25 patients with Stage II Grade B periodontitis were randomly divided into 2 groups. The experimental group was treated with SRP followed by a single subgingival application of 4% mangosteen gel, while the control group was treated with SRP followed by a single subgingival application of a placebo gel. Clinical parameters, including the plaque index (PI), the gingival bleeding index (GBI), the probing depth (PD), the relative attachment level (RAL), as well as biochemical parameters, i.e., the GCF TAOC levels, were evaluated using an ABTS (2,2'-Azino-bis-3-ethylbenzothiazoline-6-sulfonic acid) antioxidant assay kit at baseline (D0) and at 3 months (D3). RESULTS: The full-mouth PI and GBI values were significantly lower at D3 in comparison with D3. The experimental sites showed a significantly greater reduction in the PD and RAL scores as compared to control, and the GCF TAOC levels revealed a substantial rise throughout the study period, reversing from negative values at D0 to positive values at D3 in the experimental group. CONCLUSIONS: Traditional SRP with the adjunctive local delivery of 4% mangosteen gel demonstrated an added benefit in improving clinical and biochemical parameters, and thereby encouraging the use of the mangosteen gel in clinical practice for the management of moderate chronic periodontitis.

In Vitro Evaluation of Osteoblast Response to the Effect of Injectable Platelet-rich Fibrin Coating on Titanium Disks.

AIM: Biomimetic implant surface coatings can improve osteoblast response and enhance osseointegration. This study aimed to assess the response of osteoblast-like cell line (MG-63) coating of injectable platelet-rich fibrin on titanium discs. MATERIALS AND METHODS: Injectable-PRF (i-PRF) was prepared by centrifugation of blood at 700 rpm for 3 minutes without any anti-coagulant in i-PRF tubes. Ten commercially pure titanium discs were divided into control groups comprising plain discs, and test group, titanium discs were coated with i-PRF. These were then added to the cultured MG-63 cells. Cell proliferation, alkaline phosphatase production, and mineralization were assessed in both groups at day 1, 7, 14, and 21. RESULTS: The cell proliferation, alkaline phosphatase production, and mineralization increased significantly from day 1 to day 21 in both test and control groups and was significantly higher in the test group than in control group at day 1, 7, 14, and 21 (p <0.001). CONCLUSIONS: Coating of titanium discs with i-PRF causes increased proliferation, alkaline phosphatase production, and increased mineralization at day 1, 7, 14, and 21 in MG-63 osteoblast-like cells. CLINICAL SIGNIFICANCE: Improved osteoblast proliferation and mineralization demonstrate enhanced activity on the surface of an implant, which in turn may lead to increased bone to implant contact and faster/ and/or enhanced osseointegration.