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1.
J Fish Biol ; 88(3): 1204-14, 2016 Mar.
Article in English | MEDLINE | ID: mdl-26822755

ABSTRACT

Monophyly of the genus Leporinus (Characiformes: Anostomidae) was tested by sequencing and analysing a total of 4732 bp, including two mitochondrial [cytochrome oxidase subunit 1 (CO1) and cytochrome b (Cytb)] and three nuclear [myosin heavy chain 6 cardiac muscle alpha (Myh6), recombination activating gene 1 (RAG1) and recombination activating gene 2 (RAG2)] loci for 22 species of Leporinus, or c. 25% of all described species in the genus. Phylogenetic tree analyses (maximum parsimony, maximum likelihood and Bayesian species tree) indicate Leporinus to be paraphyletic, with monophyly being rejected by both Kishino-Hasegawa and Shimodaira-Hasegawa tests. The sequenced species of Leporinus are distributed across five clades that are interleaved among other anostomid genera. Several taxonomic changes are suggested as being necessary to restore monophyly for the group. The clade containing the type species, Leporinus fasciatus, should be considered Leporinus sensu stricto and at least three new genera should be described for other species currently considered part of Leporinus.


Subject(s)
Characiformes/classification , Characiformes/genetics , Genes, Mitochondrial , Phylogeny , Animals , Base Sequence , Bayes Theorem , Cytochromes b/genetics , DNA/chemistry , DNA/isolation & purification , DNA-Binding Proteins/genetics , Electron Transport Complex IV/genetics , Fresh Water , Genetic Markers , Homeodomain Proteins/genetics , Likelihood Functions , Myosin Heavy Chains/genetics , Sequence Alignment/veterinary , Sequence Analysis, DNA/veterinary
2.
J Fish Biol ; 87(3): 754-62, 2015 Sep.
Article in English | MEDLINE | ID: mdl-26212357

ABSTRACT

The aim of this study was to estimate the genetic diversity and structure of the only known population of minnow Astyanax xavante, which inhabits a stretch of river including several waterfalls. The FST values among the samples were not significant, except between two populations separated by a 30 m waterfall. Nevertheless, haplotype and nucleotide diversity increased in the downstream direction, indicating that gene flow is unidirectional, which indicates this genetic pattern as downstairs gene flow, as it has the effect of increasing genetic diversity in the downstream direction.


Subject(s)
Cyprinidae/genetics , Endangered Species , Gene Flow , Genetics, Population , Animals , Brazil , DNA, Mitochondrial/genetics , Environment , Genetic Variation , Haplotypes , Introns , Rivers , Sequence Analysis, DNA
3.
Genet Mol Res ; 13(3): 7979-91, 2014 Sep 29.
Article in English | MEDLINE | ID: mdl-25299113

ABSTRACT

Molecular genetic assessments that consider ecological information, in addition to endogamy levels, genetic diversity, and the genetic differentiation among species and populations, are particularly important for the conservation of biological diversity. Prime candidates for conservation genetic review are those subject to human use, including harvests for the ornamental fish trade. Colorful South American tetra, such as Moenkhausia oligolepis and M. forestii, are good examples of fish species that are widely collected and exported worldwide. This study aimed to evaluate the population-specific characteristics of M. oligolepis and M. forestii by comparing morphometric and molecular analyses based on ISSR markers, to provide information that would facilitate the sustainable management of these 2 species. Seventy-two specimens were collected from the Araguaia-Tocantins and Paraguay River Basins in Brazil. All specimens were measured and analyzed using ISSR markers. Population-exclusive bands were found among the 86 detected bands, while morphometric clusters reflected the geographical distribution of individuals. Correlated genetic and morphological variation supported the presence of 3 distinct groups from tributaries of the Araguaia and Mortes Rivers. Using the same techniques, all M. oligolepis populations were isolated from M. forestii. This study on Moenkhausia presents an interesting example that could be used to construct a framework of South American ichthyodiversity, and reinforces the necessity of habitat conservation to prevent the loss of biological diversity.


Subject(s)
Characidae/genetics , Conservation of Natural Resources , Genetic Variation , Animals , Characidae/classification , Genetic Markers , Polymerase Chain Reaction
4.
Genet Mol Res ; 11(2): 1610-7, 2012 Jun 15.
Article in English | MEDLINE | ID: mdl-22782580

ABSTRACT

Research has shown that genetic polymorphisms in biotransformation enzymes, such as CYP1A1 and GSTM1, are related to a greater or lesser susceptibility to various cancers. We made an analysis of CYP1A1m1 SNP and GSTM1 null genotypes in a family group (71 members) related by consanguinity who had an unusually high incidence of cancer and a high frequency of smokers. There were no significant differences in genotype frequencies in this family when compared to data for Brazilian populations. Possibly, the high incidence of cancer in this sample is associated with smoking and/or other factors not detected in this survey.


Subject(s)
Cytochrome P-450 CYP1A1/genetics , Glutathione Transferase/genetics , Neoplasms/genetics , Polymorphism, Single Nucleotide/genetics , Brazil , Female , Genetic Predisposition to Disease/genetics , Humans , Male , Pedigree
5.
Comp Cytogenet ; 6(2): 163-81, 2012.
Article in English | MEDLINE | ID: mdl-24260660

ABSTRACT

Cichlids represent one of the most species-rich families of fishes and have attracted the attention of evolutionary biologists due to the rapid radiation occurring in some groups and the importance of some species in the world aquaculture. Cytogenetic analysis was conducted in 10 cichlid species from the Araguaia River, Amazon Basin, Brazil. The chromosome number was 2n=48 for all analyzed species except for Laetacara araguaiae Ottoni et Costa, 2009 (2n=44). Chromosomal polymorphism was detected only in Geophagus proximus (Castelnau, 1855), which exhibits an extra large submetacentric and and a dot-like chromosomes. Moreover, the C-banding revealed a general pericentromeric heterochromatic pattern and some additional blocks for some species. The heterochromatic blocks corresponding to AgNOR bearing regions were observed in all species and also corresponded to CMA3 positive blocks, which were observed in terminal regions. Besides the general conserved chromosomal and heterochromatin patterns for South American cichlids, the presence of GC-rich heterochromatin was quite different in the species Biotodoma cupido (Heckel, 1840), Geophagus proximus, Retroculus lapidifer (Castelnau, 1855), Crenicichla strigata Günther, 1862 and Heros efasciatus Heckel, 1840. The results suggest that independent events of heterochromatin modification occurred during chromosome evolution in the group, regardless of the conservation of macro-chromosomal structure.

6.
J Fish Biol ; 78(4): 1239-48, 2011 Apr.
Article in English | MEDLINE | ID: mdl-21463318

ABSTRACT

Cytogenetic markers were used to compare the karyotypes of an isolated population of Hoplias malabaricus with others previously described. The results revealed peculiar characteristics that indicate a new independent evolutionary unit within the H. malabaricus complex.


Subject(s)
Chromosomes/genetics , Cytogenetic Analysis , Fishes/genetics , Genetic Variation , Animals , Female , Karyotyping , Male , Polymorphism, Genetic
7.
Cytogenet Genome Res ; 125(3): 224-34, 2009.
Article in English | MEDLINE | ID: mdl-19738382

ABSTRACT

Repeated DNA elements have been extensively applied as physical chromosome markers in comparative studies for the identification of chromosomal rearrangements, the identification of sex chromosomes, chromosome evolution analysis and applied genetics. Here, we report the characterization of the transposable elements (TE) Tc1, Rex1, Rex3 and Rex6 and a new element called RCk in the genome of the South American cichlid fish Cichla kelberi using nucleotide sequence analysis and hybridization to metaphase chromosomes. The analysis of the repeated elements demonstrated that they are, in most cases, compartmentalized in heterochromatic regions, as has been observed in several other vertebrates. On the other hand, the elements Rex1 and Rex3 were also observed spanning extensive euchromatic regions on 2 chromosome pairs. The RCk element exhibits a wide distribution among fishes and also in amphibians, and it was spread throughout the chromosomes of C. kelberi. Our results have demonstrated that the compartmentalization of repeated elements is not restricted to heterochromatic segments, which has provided new concepts with regard to the genomic organization of transposons.


Subject(s)
Cichlids/genetics , DNA Transposable Elements , Genome , Animals , Chromosomes , Cytogenetic Analysis , DNA/chemistry , DNA/genetics , Phylogeny , Physical Chromosome Mapping , Polymerase Chain Reaction , Sequence Analysis, DNA
8.
Cytogenet Cell Genet ; 56(2): 116-21, 1991.
Article in English | MEDLINE | ID: mdl-2013229

ABSTRACT

Mitotic chromosomes of four fish species of the family Anostomidae, belonging to the genera Leporinus, Leporellus, and Schizodon, were studied. With 2n = 54 meta- and sub-metacentric chromosomes, this family appears to be characterized by marked karyotypic stability. Although perceptible differences exist, mainly in the amount of constitutive heterochromatin present in the chromosomes of these species, these differences do not affect the structure and/or size of these chromosomes. Chromatin substitutions and/or modifications may have led, in one direction, to an increase in heterochromatin in some species and, in the opposite direction, to heterochromatin reduction in others. Whether these changes are accompanied by changes in the amount of euchromatin in the chromosomes is an open question. The nucleolar organizer regions, which may be located on different chromosomes in the various species, may also be indicators of reorganization of these karyotypes.


Subject(s)
Fishes/genetics , Animals , Chromosome Banding , Heterochromatin , Karyotyping , Nucleolus Organizer Region/metabolism , Species Specificity
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