ABSTRACT
Relationship of changes of methylation pattern of functionally different parts of DNA and chromosomal aberration yield was studied at the conditions of the fractionating of UV-C irradiation. Combination of restriction analysis (Hpall, MspI, MboI enzymes) with the subsequent raising of PCR (internal transcribed space ITS1, 1TS4 and inter simple sequence repeat - ISSR, 14b primers) was used. The got results testify to the changes in methylation pattern of satellite and transcription active part of DNA atan irradiation in the mode of fractionating and depending on fraction time ranges. The role of the methylation DNA pattern change in development of radiation damage and induction of organism protective reactions was discussed.
Subject(s)
Chromosome Aberrations , Chromosomes, Plant , DNA, Plant , Seedlings/radiation effects , Transcription, Genetic/radiation effects , Zea mays/radiation effects , DNA Methylation/radiation effects , DNA Primers , DNA Restriction Enzymes , DNA, Intergenic , Genetic Markers , Microsatellite Repeats , Polymerase Chain Reaction , Seedlings/genetics , Ultraviolet Rays , Zea mays/geneticsABSTRACT
Interaction between changes of functionally different DNA methylation pattern and chromosomal aberration yield under fractionated UV-C exposure at the doses-of 6.2-9 kJ/m2 have been investigated. Two ways of meristematic tissue renewal after irradiation have been revealed on the grounds of statistical analysis. To estimate changes in the methylation pattern of transcribed and satellite DNA the restriction analysis was carried out. The restriction reaction was followed by PCR with ITS1(19b), ITS4(20b) and ISSR(14b) primers. The obtained data indicate specific changes in the methylation pattern of transcribed and satellite DNA parts under fractionated irradiation with different fraction ranges. Possible role of these changes in the progressing irradiation damage and inducible protection reactions is discussed.
Subject(s)
Chromosome Aberrations/radiation effects , DNA Methylation/genetics , Zea mays/genetics , DNA Methylation/radiation effects , DNA, Satellite/genetics , DNA, Satellite/radiation effects , Ultraviolet Rays , Zea mays/radiation effectsABSTRACT
The effects of the distance interaction between the irradiated and unirradiated corn plants in the conditions of water culture were investigated. The change of biological and radiobiological reactions (radioadaptation) is shown at the helped unexposed plants, and also oppressing, as compared to the exposed homogeneous culture, similar indexes at the exposed plants from mixed culture.
Subject(s)
Adaptation, Physiological/radiation effects , Zea mays/physiology , Zea mays/radiation effects , Cobalt Radioisotopes , Seedlings/physiology , Seedlings/radiation effectsABSTRACT
Verification of hypothesis about mobile element activation radiosensitivity change in the conditions of the prolonged irradiation was the research goal. The estimation of orientation of these changes depending on the accumulated dose and their characteristic times were conducted. Two Drosophila melanogaster strains (Canton-S and P-lines) for 20 generations were in the controlled terms of chronic irradiation with 3-dose rate (1.2 x 10(-8), 0.6 x 10(-8) and 0.12 x 10(-8) Gy/c). The dynamics of hybrid dysgenese frequency was explored for each generation of F1 descendants from Canton-S and P-lines crossing. The gradual change of dose response of hybrid disgenesis depending on duration of irradiation of ancestors and dose rate was shown. The complex dynamics of hybrid dysgenese frequency depending on irradiation duration of ancestors and dose rate was shown. The cumulative effect of the prolonged irradiation shows up as adaptation at the lowest dose rate and as exhaustion at the highest dose rate. Question comes into discussion about the features of transitional process and including of protective and adaptive reactions hierarchy at the conditions of radiation factor chronic action.
Subject(s)
DNA Transposable Elements/radiation effects , Drosophila melanogaster/radiation effects , Gamma Rays , Genome, Insect/radiation effects , Adaptation, Physiological/genetics , Animals , Chimera/genetics , DNA Transposable Elements/genetics , Dose-Response Relationship, Radiation , Drosophila melanogaster/genetics , Female , Gene Frequency , MaleSubject(s)
Alcaligenes/enzymology , Polyethylene Glycols/metabolism , Pseudomonas/enzymology , Acridine Orange/pharmacology , Alcaligenes/drug effects , Alcaligenes/genetics , Biodegradation, Environmental/drug effects , Genes, Bacterial/drug effects , Mitomycin , Mitomycins/pharmacology , Plasmids/drug effects , Pseudomonas/drug effects , Pseudomonas/genetics , Pseudomonas aeruginosa/drug effects , Pseudomonas aeruginosa/enzymology , Pseudomonas aeruginosa/genetics , SewageABSTRACT
Microorganisms destroying nonionogenic surfactants (NS) were selected. The isolated cultures were assigned to the genera Pseudomonas and Bacillus. Biodestruction of 19 NS was studied in Erlenmeyer flasks with shaking and in the course of continuous cultivation. The rate of destruction was shown to depend on the chemical structure of a substance: block-copolymers of ethylene and propylene oxides were more resistant to the action of microorganisms. The results indicate that sewage waters containing NS (at an initial concentration of 50 to 500 mg/l) can be purified on local installations using microbial cultures or their complexes.
