Significance of Herbaspirillum sp. in biodegradation and biodetoxification of herbicides, pesticides, hydrocarbons and heavy metals - A review.

In today's industrialized world, contamination of soil and water with various substances has emerged as a pressing concern. Bioremediation, with its advantages of degradation or detoxification, non-polluting nature, and cost-effectiveness, has become a promising method due to technological advancements. Among the bioremediation agents, bacteria have been highly explored and documented as a productive organism. Recently, few studies have reported on the significance of Herbaspirillum sp., a Gram-negative bacterium, in bioremediating herbicides, pesticides, polycyclic aromatic hydrocarbons, metalloids, and heavy metals, as well as its role in augmenting phytoremediation efforts. Herbaspirillum sp. GW103 leached 66% of Cu from ore materials and significantly enhanced the phytoaccumulation of Pb and Zn in plumule and radical tissues of Zea mays L. plants. Additionally, Herbaspirillum sp. WT00C reduced Se6+ into Se0, resulting in an increased Se0 content in tea plants. Also, Herbaspirillum sp. proved effective in degrading 0.6 mM of 4-chlorophenol, 92.8% of pyrene, 77.4% of fluoranthene, and 16.4% of trifluralin from aqueous solution and soil-water system. Considering these findings, this review underscores the need for further exploration into the pathways of pollutant degradation, the enzymes pivotal in the degradation or detoxification processes, the influence of abiotic factors and pollutants on crucial gene expression, and the potential toxicity of intermediate products generated during the degradation process. This perspective reframes the numerical data to underscore the underutilized potential of Herbaspirillum sp. within the broader context of addressing a significant research gap. This shift in emphasis aligns more closely with the problem-necessity for solution-existing unexplored solution framework.

Detoxification of p-nitrophenol (PNP) using Enterococcus gallinarum JT-02 isolated from animal farm waste sludge.

Enterococcus gallinarum (JT-02) isolated and identified from the animal farm waste sludge was found to be capable of biodegrading p-nitrophenol (PNP), an organic compound used to manufacture drugs, fungicides, insecticides, dyes, and to darken leather. The intention of this study was to optimize the biodegradation by finding the optimal conditions for the specific strain through single-factor experiments. The bacterial strain was grown in Luria Bertani broth and various parameters were optimized to achieve the prime settings for the p-nitrophenol (PNP) biodegradation. The results indicated that the best setups for the biodegradation by the strain JT-02 was 100 mg/L of PNP; pH 7; 30 °C; 150 rpm in a shaker incubator and 3% (v/v) of inoculum dose. Once the optimal conditions were found, the bacteria were capable of degrading p-nitrophenol (98.21%) in 4 days. Intermediates produced during PNP biodegradation were identified using High Performance Liquid Chromatography (HPLC) analysis and the biodegradation pathway was elucidated. Phytotoxicity studies were carried out with Vigna radiata seeds to confirm the applicability and efficiency of PNP biodegradation.

A hybrid technique for sulfamethoxazole (SFM) removal using Enterobacter hormaechei HaG-7: Bio-electrokinetic degradation, pathway and toxicity.

Prolonged exposure to antibiotics would likely favor the development of antibiotic resistance and their gene transfer among bacterial communities that are responsible for enriched antibiotic resistant microbes. Sulfamethoxazole (SFM) is a commonly used antibiotic that is released into the environment through human and animal wastes. Improper degradation of SFM poses severe threats to mankind and all life forms. The present study aims in analyzing the process and the probability of utilizing bio-electrokinetic degradation for elimination of SFM from artificially contaminated soil employing Enterobacter hormaechei HaG-7. The desired optimal conditions for SFM degradation (∼98%) were observed at SFM initial concentration (100 mg/L) with an inoculum dose (1% v/v) and applied potential voltage (1.5 V) at pH (7). The results indicated efficient and complete degradation of SFM when compared with the conventional biodegradation.

Enhanced visible light-driven photocatalysis of iron-oxide/titania composite: Norfloxacin degradation mechanism and toxicity study.

A simulated visible light-mediated iron oxide-titania (IoT) nanocomposite was employed to degrade the antibiotic norfloxacin (NFN) photocatalytically. The photocatalyst were prepared using a sol-gel method with controlled titania loadings to iron oxide by altering the fabrications step. The nanocomposites were structurally characterized by field emission scanning electron microscopy (FE-SEM), energy-dispersive X-ray spectroscopy (EDX), field emission high-resolution transmission electron microscopy (HR-TEM), X-ray diffraction (XRD), Fourier transform infrared (FT-IR) spectroscopy, Diffuse reflectance UV-visible spectra (DRS-UV) spectroscopy, cyclic voltammetry, and X-ray photoelectron spectroscopy (XPS). It was observed that 100 mg/L of iron oxide doped titania loading at 1:4 (IoT-4) achieved the maximum photocatalytic activity in a 75 mg/100 mL of NFN solution within 60 min of the reaction time under visible light irradiation. The NFN degradation mechanism affirmed using HPLC-MS/MS analysis and the results confirmed the complete NFN degradation without residual intermediates. Significant, sustained recyclability was obtained by completely removing the contaminant up to 5 cycles with 90% degradation ability till nine cycles. Bacterial- and phytotoxicity data ascertain that the photocatalyzed and contaminant-free water is safe for the environment. The outstanding photocatalytic performance in removing organic pollutants indicates the potential application of IoT nanocomposites in real-time environmental remediation.

Enhanced visible light-driven photocatalytic activity of reduced graphene oxide/cadmium sulfide composite: Methylparaben degradation mechanism and toxicity.

Reduced graphene oxide/cadmium sulfide (RGOCdS) nanocomposite synthesized through solvothermal process was used for methylparaben (MeP) degradation. The crystallinity of the nanocomposite was ascertained through X-ray diffraction. High resolution transmission electron microscope (HRTEM) results proved the absence of any free particle beyond the catalyst surface ensuring the composite nature of the prepared material. The enhancement in the activity on doping with RGO was substantiated by diffuse reflectance spectroscopy (DRS-UV). It is evident from the photocatalytic degradation experiments that RGOCdS is more efficient than pure CdS. Maximum MeP degradation (100%) was achieved after 90 min of irradiation with 750 mg/L RGOCdS dosage at an acidic pH of 3, for an initial MeP concentration of 30 mg/L. The degradation mechanism substantiated through HPLC-MS/MS analysis showed the complete degradation of MeP without any residual intermediaries. The catalyst could be sustained and reused for up to 9 cycles of usage. Phytotoxicity and mycotoxicity results evidently ascertain the environmental implications of the photocatalyst material.