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1.
Transl Psychiatry ; 4: e351, 2014 Jan 28.
Article in English | MEDLINE | ID: mdl-24473442

ABSTRACT

Stroke survivors often experience social isolation. Social interaction improves quality of life and decreases mortality after stroke. Male mice (20-25 g; C57BL/6N), all initially pair housed, were subjected to middle cerebral artery occlusion (MCAO). Mice were subsequently assigned into one of three housing conditions: (1) Isolated (SI); (2) Paired with their original cage mate who was also subjected to stroke (stroke partner (PH-SP)); or (3) Paired with their original cage mate who underwent sham surgery (healthy partner (PH-HP)). Infarct analysis was performed 72 h after stroke and chronic survival was assessed at day 30. Immediate post-stroke isolation led to a significant increase in infarct size and mortality. Interestingly, mice paired with a healthy partner had significantly lower mortality than mice paired with a stroke partner, despite equivalent infarct damage. To control for changes in infarct size induced by immediate post-stroke isolation, additional cohorts were assessed that remained pair housed for three days after stroke prior to randomization. Levels of brain-derived neurotrophic factor (BDNF) were assessed at 90 days and cell proliferation (in cohorts injected with 5-bromo-2'-deoxyuridine, BrdU) was evaluated at 8 and 90 days after stroke. All mice in the delayed housing protocol had equivalent infarct volumes (SI, PH-HP and PH-SP). Mice paired with a healthy partner showed enhanced behavioral recovery compared with either isolated mice or mice paired with a stroke partner. Behavioral improvements paralleled changes in BDNF levels and neurogenesis. These findings suggest that the social environment has an important role in recovery after ischemic brain injury.


Subject(s)
Behavior, Animal/physiology , Infarction, Middle Cerebral Artery/rehabilitation , Interpersonal Relations , Neurogenesis/physiology , Social Isolation , Animals , Brain-Derived Neurotrophic Factor/metabolism , Hippocampus/metabolism , Infarction, Middle Cerebral Artery/complications , Infarction, Middle Cerebral Artery/pathology , Male , Mice , Mice, Inbred C57BL , Random Allocation , Recovery of Function/physiology
2.
Neuroscience ; 201: 280-7, 2012 Jan 10.
Article in English | MEDLINE | ID: mdl-22120436

ABSTRACT

Ischemic preconditioning (IPC) induces endogenous neuroprotection from a subsequent ischemic injury. IPC involves downregulation of metabolic pathways. As adenosine 5'-monophosphate-activated protein kinase (AMPK) is a critical sensor of energy balance and plays a major role in cellular metabolism, its role in IPC was investigated. A brief 3-min middle cerebral artery occlusion (MCAO) was employed to induce IPC in male mice 72 h before 90-min MCAO. Levels of AMPK and phosphorylated AMPK (pAMPK), the active form of the kinase, were assessed after IPC. A pharmacological activator or inhibitor of AMPK was used to determine the dependence of IPC on AMPK signaling. Additionally, AMPK-α2 null mice were subjected to IPC, and subsequent infarct damage was assessed. IPC induced neuroprotection, enhanced heat shock protein-70 (HSP-70), and improved behavioral outcomes. These beneficial effects occurred in parallel with a significant inhibition of pAMPK protein expression. Although both pharmacological inhibition of AMPK or IPC led to neuroprotection, IPC offered no additional protective effects when co-administered with an AMPK inhibitor. Moreover, pharmacological activation of AMPK with metformin abolished the neuroprotective effects of IPC. AMPK-α2 null mice that lack the catalytic isoform of AMPK failed to demonstrate a preconditioning response. Regulation of AMPK plays an important role in IPC-mediated neuroprotection. AMPK may be a potential therapeutic target for the treatment of cerebral ischemia.


Subject(s)
Brain/enzymology , Down-Regulation/physiology , Infarction, Middle Cerebral Artery/prevention & control , Ischemic Preconditioning/methods , Phosphotransferases (Alcohol Group Acceptor)/metabolism , Animals , Brain/pathology , Brain Infarction/etiology , Brain Infarction/pathology , Brain Infarction/prevention & control , Disease Models, Animal , Fluoresceins , HSP72 Heat-Shock Proteins/metabolism , Indoles , Infarction, Middle Cerebral Artery/complications , Male , Mice , Mice, Inbred C57BL , Nervous System Diseases/etiology , Neurologic Examination , Organic Chemicals , Statistics, Nonparametric , Time Factors
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