In vitro activity of essential oils extracted from condiments against fluconazole-resistant and -sensitive Candida glabrata.

In the present study, the antifungal activity of essential oils obtained from Origanum vulgare (oregano), Cinnamomum zeylanicum (cinnamon), Lippia graveolens (Mexican oregano), Thymus vulgaris (thyme), Salvia officinalis (sage), Rosmarinus officinalis (rosemary), Ocimum basilicum (basil) and Zingiber officinale (ginger) were assessed against Candida glabrata isolates. One group contained 30 fluconazole-susceptible C. glabrata isolates, and the second group contained fluconazole-resistant isolates derived from the first group after the in vitro induction of fluconazole-resistance, for a total of 60 tested isolates. The broth microdilution methodology was used. Concentrations of 50µg/mL, 100µg/mL, 200µg/mL, 400µg/mL, 800µg/mL, 1600µg/mL and 3200µg/mL of the essential oils were used, and the minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) were determined. Thyme, sage, rosemary, basil and ginger essential oils showed no antifungal activity at the tested concentrations. Antimicrobial activity less than or equal to 3200µg/mL was observed for oregano, Mexican oregano and cinnamon essential oils. Both the oregano and Mexican oregano essential oils showed high levels of antifungal activity against the fluconazole-susceptible C. glabrata group, whereas the cinnamon essential oil showed the best antifungal activity against the fluconazole-resistant C. glabrata isolates.

Recria de bovinos de corte mantidos em pastagem de aveia preta com diferentes ofertas de forragem, com e sem suplementação / Rearing of beef cattle grazing oats with different forage allowances, with or without supplementation

O objetivo deste estudo foi avaliar o desempenho de bezerros recebendo ou não suplemento energético, mantidos em pastagem de aveia preta (Avena strigosa Schreb) manejada sob duas ofertas de forragem. Foram utilizados 16 animais do grupamento genético Composto Marchangus Z (1/4 Marchigiana 1/4 Angus 1/2 Nelore), com oito meses de idade, castrados, e com peso médio inicial de 200kg. O delineamento experimental utilizado foi o inteiramente ao acaso, em um fatorial 2x2 (duas ofertas de forragem x dois manejos alimentares - com e sem suplementação de milho moído). Não houve interação significativa entre oferta e suplementação para nenhuma das variáveis. A oferta de forragem real ficou próxima do estipulado para 6 e 10% (5,95% e 9,07%, respectivamente). A oferta de 6% apresentou maior porcentagem de folha (57,0 contra 30,4%), menor porcentagem de colmo (43,0 contra 69,6%) e, consequentemente, maior relação folha/colmo (1,45 contra 0,45), resultando em pastagens de melhor qualidade (PB = 20,0 contra 13,6%) na menor oferta. A taxa de acúmulo diária foi maior (P<0,05) para oferta de forragem de 6% (34,2kg de MS/ha/dia) em relação à oferta de 10% (28,1kg de MS/ha/dia). A suplementação proporcionou maior ganho de peso médio diário (0,749 contra 0,594kg/dia) na recria de bezerros em pastagem de aveia preta IPR-61.

The objective of this study was to evaluate the performance of calves fed supplemented energy or not maintained in oat (Avena strigosa Schreb) managed under two forage allowances. Sixteen Marchangus (1/4 Marchigiana 1/4 Angus 1/2 Nelore) animals were used at eight months old and average initial weight of 200kg. The experimental design utilized was completely randomized in a 2x2 factorial (two forage allowances x two feeding - with and without supplementation of corn). There was no significant interaction between supply and supplementation for the variables. The real forage allowance was close to the expected from 6 to 10% (5.95 and 9.07%, respectively). The offer of 6% shows higher percentage of leaf (57.0 versus 30.4%), lower percentage of stem (43.0 versus 69.6%) and, consequently, higher leaf/stem ratio (1.45 versus 0.45), resulting in better forage (PB=20.0 versus 13.6%), in lower offer. The accumulated rate was higher (P<.05) for forage allowance of 6% (34.2kg of MS/ha/day) in relation to the offer of 10% (28,1kg of MS/ha/day). Supplemented animals showed higher average dairy weight (.749 versus .549kg/day).

In vitro interactions between amphotericin B and other antifungal agents and rifampin against Fusarium spp.

Fusarium species are common hyaline soil saprophytes and plant pathogens that are opportunistic fungal pathogens of immunocompromised patients. The treatment for fusariosis remains uncertain with an unfavourable prognosis; new possibilities for treatment, such as various synergistic drug interactions, must be uncovered. In this study, we evaluated the in vitro interactions of amphotericin B with caspofungin, ketoconazole, 5-flucytosine, itraconazole, miconazole, rifampin, fluconazole, terbinafine and voriconazole against isolates of Fusarium spp. using the chequerboard method with interactions evaluated by fractional inhibitory concentration indices. The highest percentages of synergistic interactions were observed for the combinations of amphotericin B and caspofungin (68.7%), amphotericin B and rifampin (68.7%), amphotericin B plus 5-flucytosine (59.3%) and amphotericin B with voriconazole (37.5%). The pattern of susceptibility to antifungal agents among Fusarium species and their consequence on the effects of drug combinations are also discussed.