Biosolid compost with wood shavings and yard trimmings alleviates stress and improves grain quality in soybean grown in lead polluted soils.

Heavy metals induce stress in plants, thereby affecting growth, crop quality, and food security. Most studies addressing the mitigation of these effects by soil amendment have focused on metals in soils and plant uptake, with there still being a great deal of uncertainty about how amendment application in polluted soils can modify plant stress response and, consequently, yield and food safety. Thus, the aim of this study was to evaluate the effect of biosolid compost amendment on stress response, growth, and lead accumulation in Glycine max, when applied to lead polluted agricultural soils. Soybean was grown in lead polluted soils with 0%, 5%, or 10% (w/w) biosolid compost amendment under controlled conditions in a greenhouse, and the stress response indicators chlorophylls, proteins, sugars, malondialdehyde, glutathione S-transferase activity, carotenes, and the ferric reducing antioxidant power were investigated. In addition, the biomass and lead accumulation in different organs were determined and evaluated with respect to the plant stress. Our results revealed that the addition of 10% biosolid compost improved the grain biomass and appeared to reduce the amount of defective grains, which was related to higher Pb concentrations. Furthermore, 10% compost treatment reduced the stress in plants, leading to a better performance of the photosynthetic system, and with the antioxidant response being positively correlated to Pb accumulation. Lead uptake in plants was decreased by between 35 and 57% after this treatment in comparison with unamended soils. These results indicate that biosolid compost amendment may be an effective way to alleviate Pb uptake and metal stress in soybeans.

Response of tungsten (W) solubility and chemical fractionation to changes in soil pH and soil aging.

A thorough understanding of the geochemical behavior of W in soils is crucial for environmental risk assessment. Soil pH is known as master variable of element solubility and bioavailability in soils. Here we report on effects of soil pH (modified by liming and acid - base additions) and soil aging on the environmental availability of W in soil using W solubility and chemical fractionation as indicators. Experimental soils included two naturally acidic soils with contrasting soil texture (SAND, CLAY), at native pH or limed with 2.5% CaCO3, and spiked with increasing concentration of W. Our results showed that W was significantly more labile in alkaline compared to acidic soils, confirming the validity of results of pure-mineral studies for more heterogeneously composed soils. While labile W was generally greater in the SAND compared to the CLAY soil, the reverse trend was observed in the limed soils at the highest W addition (5000 mg kg-1). Combining our results with previous mechanistic reports suggests that clay edge sorption sites significantly contributed to W retention in treatments with low to medium W additions, resulting in lower environmental availability for W in the CLAY soil. At high W concentrations and high pH, the stronger W retention in the SAND was attributed to continuous formation of W surface polymers on the more abundant metal (oxyhydr)oxides, a process that has been previously reported to occur even under alkaline conditions. A first comparison of various soil chemical methods (Bray & AB-DTPA extractions, soil solution centrifugation CL, diffusion-based DGT) to predict W phytoavailability in soil also revealed a strong pH dependency challenging the identification of a suitable method. This study is one of the first demonstrating the pH dependence of W in natural soils and delivers evidence for increased risk of W mobilization in W polluted, alkaline soil environments.

Effects of co-cropping on soybean growth and stress response in lead-polluted soils.

Phytoremediation by co-cropping may be a promising approach to produce safe crops while remediating the soil. However, the effects of plant interaction, especially stress response, remain unclear. The aims of this study were to investigate the effect of co-cropping on plant growth, stress response and lead (Pb) uptake in soybean and Tagetes minuta, and to assess the feasibility of agricultural production in Pb-polluted soils. A pot experiment was conducted to study the effect of co-cropping vs monocrop at three soil Pb concentrations. The following parameters were analyzed: biomass, Pb content in plants, and stress response indicators (chlorophylls, proteins, sugars, malondialdehyde, glutathione S-transferase activity, carotenes and antioxidant power). Results showed that in co-cropping, both species were benefited in polluted soils, since biomass and stress response were improved. T. minuta reduced adverse effects of Pb on soybean by improving grain quality and even survival in polluted soils, where soybean in monocrop grew only up to early vegetative stages. This effect was related to a 50% reduction in lipid peroxidation for soybean in co-cropping along with a sharp increase in the antioxidant response. In addition, co-cropping enhanced Pb accumulation in T. minuta (45% higher), as well as content of chlorophylls and carotenes (66% and 42% of increment, respectively) and glutathione S-transferase activity (two times higher) in the highly polluted soil. Our results showed that rhizosphere interactions can help enhance tolerance to Pb toxicity in both species, allowing soybean production in highly polluted soils without posing health risk from grain consumption.

Metal solubility in the rhizosphere of a co-cropping system. The role of total carbon exudation, soluble proteins and plant interaction.

In the present study we assessed how modified rhizosphere pH and root exudation (total carbon (C) and soluble proteins released) affected lead (Pb) solubility as well as plant growth and Pb accumulation. A pot experiment with Pb polluted agricultural soils was performed, which involved growing two species, Capsicum annum (pepper) and Tagetes minuta, with the latter being a native herb indicated as potential phytoextractor of Pb, in monocrop and co-cropping conditions. Changes in plant growth, metal uptake as well as rhizosphere soil parameters (pH, EC) and total C and protein exudation were determined. In addition, the metal extraction efficiency of exudates released under mono- and co-cropped conditions were investigated. Results showed that in contrast to the control soil (with low Pb concentration), total C exudation was higher in co-cropping systems in Pb contaminated soils which lead to increases in Pb uptake in both species. Exudates originating from T. minuta were more efficient in solubilizing Pb than exudates from pepper when grown under mono-cropping conditions. Exudates derived from co-cropping both species were either equally or less efficient in mobilizing Pb than exudates from T. minuta. The capacity of exudates to mobilize metals was dependent not only on the species specific quality of root exudates released, but also on its quantity, with the metal extraction efficiency increasing with C concentration in exudates. However, the role of exuded proteins in Pb solubilization was found to be negligible. Biochemical interactions in the rhizosphere under co-cropping conditions favored metal solubilization, and consequently Pb accumulation. The co-cropping conditions could allow accumulation of Pb to levels in pepper that pose risks when the plants are used as a food source.

Availability of lead in agricultural soils amended with compost of biosolid with wood shavings and yard trimmings.

Lead-polluted agricultural soils are a serious problem for food safety, with organic amendment being a promising mitigation method from the environmental perspective. Therefore, the purpose of this study was to evaluate lead availability and the effectiveness of the application of compost of biosolid with wood shavings and yard trimmings in contaminated soils. The physicochemical (Pb distribution, organic matter, pH, electric conductivity, cation exchange capacity, nitrogen, phosphorus, carbon, carbonates, exchangeable cations, sodium) and biological parameters (the microbial activity obtained by fluorescein diacetate hydrolysis) in Pb-polluted and non-polluted agricultural soils were evaluated after the addition of biosolid with wood shavings and yard trimming compost. Topsoils (lead-polluted and control) were collected in the vicinity of a former battery-recycling plant, amended with compost (0%, 5%, and 10%), and incubated in controlled conditions for 118 days. The results showed that lead availability decreased significantly, and the nutritional quality of the soils increased in the soils amended with 10% of compost. Taken together, the results of the present study indicated that compost amendment could be an effective method for mitigating the negative effects of lead in agricultural soils.

pH-Dependent Bioavailability, Speciation, and Phytotoxicity of Tungsten (W) in Soil Affect Growth and Molybdoenzyme Activity of Nodulated Soybeans.

Increasing use of tungsten (W)-based products opened new pathways for W into environmental systems. Due to its chemical alikeness with molybdenum (Mo), W is expected to behave similarly to its "twin element", Mo; however, our knowledge of the behavior of W in the plant-soil environment remains inadequate. The aim of this study was to investigate plant growth as well as W and nutrient uptake depending on soil chemical properties such as soil pH and texture. Soybean ( Glycine max cv. Primus) was grown on two acidic soils differing in soil texture that were either kept at their natural soil pH (pH of 4.5-5) or limed (pH of ≥7) and amended with increasing concentrations of metallic W (control and 500 and 5000 mg kg-1). In addition, the activity of molybdoenzymes involved in N assimilation (nitrate reductase) and symbiotic N2 fixation (nitrogenase) was also investigated. Our results showed that the risk of W entering the food web was significantly greater in high-pH soils due to increased solubility of mainly monomeric W. The effect of soil texture on W solubility and phytoavailability was less pronounced compared to soil pH. Particularly at intermediate W additions (W 500 mg kg-1), symbiotic nitrogen fixation was able to compensate for reduced leaf nitrate reductase activity. When W soil solution concentrations became too toxic (W 5000 mg kg-1), nodulation was more strongly inhibited than nitrogenase activity in the few nodules formed, suggesting a more-efficient detoxification and compartmentalization mechanism in nodules than in soybean leaves. The increasing presence of polymeric W species observed in low-pH soils spiked with high W concentrations resulted in decreased W uptake. Simultaneously, polymeric W species had an overall negative effect on nutrient assimilation and plant growth, suggesting a greater phytotoxicity of W polymers. Our study demonstrates the importance of accounting for soil pH in risk assessment studies of W in the plant-soil environment, something that has been completely neglected in the past.

Spatial patterns of high Aedes aegypti oviposition activity in northwestern Argentina.

BACKGROUND: In Argentina, dengue has affected mainly the Northern provinces, including Salta. The objective of this study was to analyze the spatial patterns of high Aedes aegypti oviposition activity in San Ramón de la Nueva Orán, northwestern Argentina. The location of clusters as hot spot areas should help control programs to identify priority areas and allocate their resources more effectively. METHODOLOGY: Oviposition activity was detected in Orán City (Salta province) using ovitraps, weekly replaced (October 2005-2007). Spatial autocorrelation was measured with Moran's Index and depicted through cluster maps to identify hot spots. Total egg numbers were spatially interpolated and a classified map with Ae. aegypti high oviposition activity areas was performed. Potential breeding and resting (PBR) sites were geo-referenced. A logistic regression analysis of interpolated egg numbers and PBR location was performed to generate a predictive mapping of mosquito oviposition activity. PRINCIPAL FINDINGS: Both cluster maps and predictive map were consistent, identifying in central and southern areas of the city high Ae. aegypti oviposition activity. A logistic regression model was successfully developed to predict Ae. aegypti oviposition activity based on distance to PBR sites, with tire dumps having the strongest association with mosquito oviposition activity. A predictive map reflecting probability of oviposition activity was produced. The predictive map delimitated an area of maximum probability of Ae. aegypti oviposition activity in the south of Orán city where tire dumps predominate. The overall fit of the model was acceptable (ROC=0.77), obtaining 99% of sensitivity and 75.29% of specificity. CONCLUSIONS: Distance to tire dumps is inversely associated with high mosquito activity, allowing us to identify hot spots. These methodologies are useful for prevention, surveillance, and control of tropical vector borne diseases and might assist National Health Ministry to focus resources more effectively.

Landscape determinants of Saint Louis encephalitis human infections in Córdoba city, Argentina during 2010.

Saint Louis encephalitis virus (SLEV) is endemic in Argentina. During 2005 an outbreak occurred in Córdoba. From January to April of 2010 a new outbreak occurred in Córdoba city with a lower magnitude than the one reported in 2005. Understanding the association of different landscape elements related to SLEV hosts and vectors in urban environments is important for identifying high risk areas for human infections, which was here evaluated. The current study uses a case-control approach at a household geographical location, considering symptomatic and asymptomatic human infections produced by SLEV during 2010 in Córdoba city. Geographical information systems and logistic regression analysis were used to study the distribution of infected human cases and their proximity to water bodies, vegetation abundance, agricultural fields and housing density classified as high/low density urban constructions. Population density at a neighborhood level was also analyzed as a demographic variable. Logistic regression analysis revealed vegetation abundance was significantly (p<0.01) associated with the presence of human infections by SLEV. A map of probability of human infections in Córdoba city was derived from the logistic model. The model highlights areas that are more likely to experience SLEV infections. Landscape variables contributing to the outbreak were the proximity to places with vegetation abundance (parks, squares, riversides) and the presence of low density urban constructions, like residential areas. The population density analysis shows that SLEV infections are more likely to occur when population density by neighborhood is lower. These findings and the predictive map developed could be useful for public health surveillance and to improve prevention of vector-borne diseases.