Background-suppressed SRS fingerprint imaging with a fully integrated system using a single optical parametric oscillator.

Stimulated Raman Scattering (SRS) imaging can be hampered by non-resonant parasitic signals that lead to imaging artifacts and eventually overwhelm the Raman signal of interest. Stimulated Raman gain opposite loss detection (SRGOLD) is a three-beam excitation scheme capable of suppressing this nonlinear background while enhancing the resonant Raman signal. We present here a compact electro-optical system for SRGOLD excitation which conveniently exploits the idler beam generated by an optical parametric oscillator (OPO). We demonstrate its successful application for background suppressed SRS imaging in the fingerprint region. This system constitutes a simple and valuable add-on for standard coherent Raman laser sources since it enables flexible excitation and background suppression in SRS imaging.

Intravascular optical coherence tomography to validate finite-element simulation of angioplasty balloon inflation.

Concrete methods are lacking to examine angioplasty simulation results. For the first time, we explored the application of intravascular optical coherence tomography (IVOCT) to experimentally validate results obtained from finite-element simulation of angioplasty balloon deployment. In order to simulate each experimental scenario, IVOCT images were used to create initial geometrical models for the balloon and the phantoms. The study comprised three scenarios. The first scenario involved experimentally monitoring as well as simulating free expansion of the balloon. The second scenario involved experimentally monitoring as well as simulating balloon inflation inside three artery phantoms with different mechanical properties. The third scenario involved experimentally monitoring as well as simulating balloon unfolding and inflation inside a multilayer phantom. Using the first scenario, a constitutive model was developed for the balloon and was tuned to fit the IVOCT balloon inflation monitoring results. This model was used to simulate the balloon's response in simulations involving phantoms corresponding to the second and third scenarios. Diameter values were calculated both from images and simulation results. These values were then compared for each scenario. The obtained results highlight the potentials of IVOCT monitoring to validate simulation procedures.

Assessment of Compressive Raman versus Hyperspectral Raman for Microcalcification Chemical Imaging.

We experimentally implement a compressive Raman technology (CRT) that incorporates chemometric analysis directly into the spectrometer hardware by means of a digital micromirror device (DMD). The DMD is a programmable optical filter on which optimized binary filters are displayed. The latter are generated with an algorithm based on the Cramer-Rao lower bound. We compared the developed CRT microspectrometer with two conventional state-of-the-art Raman hyperspectral imaging systems on samples mimicking microcalcifications relevant for breast cancer diagnosis. The CRT limit of detection significantly improves, when compared to the CCD based system, and CRT ultimately allows 100× and 10× faster acquisition speeds than the CCD- and EMCCD-based systems, respectively.

Diazonium Salt-Based Surface-Enhanced Raman Spectroscopy Nanosensor: Detection and Quantitation of Aromatic Hydrocarbons in Water Samples.

Here, we present a surface-enhanced Raman spectroscopy (SERS) nanosensor for environmental pollutants detection. This study was conducted on three polycyclic aromatic hydrocarbons (PAHs): benzo[a]pyrene (BaP), fluoranthene (FL), and naphthalene (NAP). SERS substrates were chemically functionalized using 4-dodecyl benzenediazonium-tetrafluoroborate and SERS analyses were conducted to detect the pollutants alone and in mixtures. Compounds were first measured in water-methanol (9:1 volume ratio) samples. Investigation on solutions containing concentrations ranging from 10-6 g L-1 to 10-3 g L-1 provided data to plot calibration curves and to determine the performance of the sensor. The calculated limit of detection (LOD) was 0.026 mg L-1 (10-7 mol L-1) for BaP, 0.064 mg L-1 (3.2 × 10-7 mol L-1) for FL, and 3.94 mg L-1 (3.1 × 10-5 mol L-1) for NAP, respectively. The correlation between the calculated LOD values and the octanol-water partition coefficient (Kow) of the investigated PAHs suggests that the developed nanosensor is particularly suitable for detecting highly non-polar PAH compounds. Measurements conducted on a mixture of the three analytes (i) demonstrated the ability of the developed technology to detect and identify the three analytes in the mixture; (ii) provided the exact quantitation of pollutants in a mixture. Moreover, we optimized the surface regeneration step for the nanosensor.

Demonstration of a frequency spectral compression effect through an up-conversion interferometer.

This paper reports on the experimental implementation of an interferometer featuring sum frequency generation (SFG) processes powered by a pump spectral doublet. The aim of this configuration is to allow the use of the SFG process over an enlarged spectral domain. By analyzing the converted signal, we experimentally demonstrate a frequency spectral compression effect from the infrared input signal to the visible one converted through the SFG process. Recently, such a compression effect has been numerically demonstrated by Wabnitz et al. We also verify experimentally that we fully retrieve the temporal coherence properties of the infrared input signal in the visible field. The experimental setup permits to demonstrate an experimental frequency spectral compression factor greater than 4. This study takes place in the general field of coherence analysis through second order non-linear processes.

Intravascular optical coherence tomography to characterize tissue deformation during angioplasty: preliminary experiments with artery phantoms.

We explored the potential of intravascular optical coherence tomography (IVOCT) to assess deformation during angioplasty balloon inflation. Using a semi-compliant balloon and artery phantoms, we considered two experimental scenarios. The goal for the first scenario was to investigate if variation in the elasticity of the structure surrounding the balloon could be sensed by IVOCT monitoring. In this scenario, we used three single-layer phantoms with various mechanical properties. Image analysis was performed to extract the inner and outer diameters of the phantoms at various pressures. The goal for the second scenario was twofold. First, we investigated the IVOCT capability to monitor a more complex balloon inflation process. The balloon was in a folded state prior to inflation. This allowed studying two stages of deformation: during balloon unfolding and during balloon expansion. Second, we investigated IVOCT capability to monitor the deformation in a three-layer phantom used to better mimic a true artery. So, not only were the IVOCT images processed to provide the inner and outer diameters of the phantom, but the layer thicknesses were also determined. In both scenarios, IVOCT monitoring revealed to be very efficient in providing relevant information about the phantom deformation during balloon inflation.

Optimal signal processing of nonlinearity in swept-source and spectral-domain optical coherence tomography.

We demonstrate the efficiency of the convolution using an optimized Kaiser-Bessel window to resample nonlinear data in wavenumber for Fourier-domain optical coherence tomography (OCT). We extend our previous experimental demonstration that was performed with a specific swept-source nonlinearity. The method is now applied to swept-source OCT data obtained for various simulated swept-source nonlinearities as well as spectral-domain OCT data obtained from both simulations and experiments. Results show that the new optimized method is the most efficient for handling all the different types of nonlinearities in the wavenumber domain that one can encounter in normal practice. The efficiency of the method is evaluated through comparison with common methods using resampling through interpolation prior to performing a fast-Fourier transform and with the accurate but time-consuming discrete Fourier transform for unequally spaced data, which involves Vandermonde matrices.

Real-time control of angioplasty balloon inflation based on feedback from intravascular optical coherence tomography: experimental validation on an excised heart and a beating heart model.

We report on real-time control of balloon inflation inside porcine arteries. In the first step, experiments were done in a coronary artery of an excised heart. In the second step, experiments were done in a beating heart setup providing conditions very close to in vivo conditions without the complications. A programmable syringe pump was used to inflate a compliant balloon in arteries, while intravascular optical coherence tomography (IVOCT) monitoring was performed. In a feedback loop, IVOCT images were processed to provide the balloon diameter values in real time to control the pump action in order to achieve a target diameter. In different experiments, various flow rates and target diameters were used. In the excised heart experiment, there was good convergence to target diameters resulting in a satisfactory balloon inflation control. In the beating heart experiment, there were oscillations in the diameter values due to cyclic arterial contractions. In these experiments, the control system maintained diameter averages satisfactorily close to predetermined target values. Real-time control of balloon inflation could not only provide a safer outcome for angioplasty procedures, but could also provide additional information for diagnostics since it implicitly provides information about the artery response to the inflation process.

Real-time control of angioplasty balloon inflation based on feedback from intravascular optical coherence tomography: preliminary study on an artery phantom.

A method is proposed to achieve computerized control of angioplasty balloon inflation, based on feedback from intravascular optical coherence tomography (IVOCT). Controlled balloon inflation could benefit clinical applications, cardiovascular research, and medical device industry. The proposed method was experimentally tested for balloon inflation within an artery phantom. During balloon inflation, luminal contour of the phantom was extracted from IVOCT images in real time. Luminal diameter was estimated from the obtained contour and was used in a feedback loop. Based on the estimated actual diameter and a target diameter, a computer controlled a programmable syringe pump to deliver or withdraw liquid in order to achieve the target diameter. The performance of the control method was investigated under different conditions, e.g., various flow rates and various target diameters. The results were satisfactory, as the control method provided convergence to the target diameters in various experiments.

Optical coherence tomography monitoring of angioplasty balloon inflation in a deployment tester.

We present an innovative integration of an intravascular optical coherence tomography probe into a computerized balloon deployment system to monitor the balloon inflation process. The high-resolution intraluminal imaging of the balloon provides a detailed assessment of the balloon quality and, consequently, a technique to improve the balloon manufacturing process. A custom-built swept-source optical coherence tomography system is used for real-time imaging. A semicompliant balloon with a nominal diameter of 4 mm is fabricated for the experiments. Imaging results correspond to balloon deployment in air and inside an artery phantom. A characterization of the balloon diameter, wall thickness, compliance, and elastic modulus is provided, based on image segmentation. Using the images obtained from the probe pullback, a three-dimensional visualization of the inflated balloon is presented.

Intravascular optical coherence tomography on a beating heart model.

The advantages and limitations of using a beating heart model in the development of intravascular optical coherence tomography are discussed. The model fills the gap between bench experiments, performed on phantoms and excised arteries, and whole animal in-vivo preparations. The beating heart model is stable for many hours, allowing for extended measurement times and multiple imaging sessions under in-vivo conditions without the complications of maintaining whole-animal preparation. The perfusate supplying the heart with nutrients can be switched between light scattering blood to a nonscattering perfusate to allow the optical system to be optimized without the need of an efficient blood displacement strategy. Direct access to the coronary vessels means that there is no need for x-ray fluoroscopic guidance of the catheter to the heart, as is the case in whole animal preparation. The model proves to be a valuable asset in the development of our intravascular optical coherence tomography technology.

Experimental validation of an optimized signal processing method to handle non-linearity in swept-source optical coherence tomography.

We evaluate various signal processing methods to handle the non-linearity in wavenumber space exhibited by most laser sources for swept-source optical coherence tomography. The following methods are compared for the same set of experimental data: non-uniform discrete Fourier transforms with Vandermonde matrix or with Lomb periodogram, resampling with linear interpolation or spline interpolation prior to fast-Fourier transform (FFT), and resampling with convolution prior to FFT. By selecting an optimized Kaiser-Bessel window to perform the convolution, we show that convolution followed by FFT is the most efficient method. It allows small fractional oversampling factor between 1 and 2, thus a minimal computational time, while retaining an excellent image quality.

Artifact removal in Fourier-domain optical coherence tomography with a piezoelectric fiber stretcher.

We describe an artifact removal setup swept-source optical coherence tomography (OCT) system that enables high-speed full-range imaging. We implement a piezoelectric fiber stretcher to generate a periodic phase shift between successive A-scans, thus introducing a transverse modulation. The depth ambiguity is then resolved by performing a Fourier filtering in the transverse direction before processing the data in the axial direction. The dc artifact is also removed. The key factor is that the piezoelectric fiber stretcher can be used to generate discrete phase shifts with a high repetition rate. The proposed experimental setup is a much improved version of the previously reported B-M mode scanning for spectral-domain OCT in that it does not generate additional artifacts. It is a simple and low-cost solution for artifact removal that can easily be applied.

Three-beam photonic crystal fiber imaging interferometer.

Some varieties of photonic crystal fiber (PCF) have huge potential for high-resolution imaging in astronomy. They allow us to operate in a single-mode regime over a wide bandwidth keeping a high transmission level. Thus they may be used to carry wide spectral light over hundreds of meters. We report the implementation of what we believe to be the first three-beam interferometer fitted with PCF arms. This configuration is needed to achieve high-resolution imaging. A full description of the experimental setup is given, and closure phase measurements achieved over a 1 microm bandwidth are also presented. With a pointlike source, the theoretical closure phase is expected to be 0 rad. Experimentally, a mean closure phase of 0.01 rad has been measured with a 0.07 rad standard deviation. These results confirm that PCF should be used in an astronomical context.

Characterization of fluoride fibers for the Optical Hawaiian Array for Nanoradian Astronomy project.

We report on the interferometric characterization of a pair of 300 m long single-mode non-polarization-maintaining fibers designed for the Optical Hawaiian Array for Nanoradian Astronomy ('OHANA) project whose goal is to realize a kilometric near-infrared astronomical array by connecting the large telescopes of the Mauna Kea observatory with single-mode fibers. The fluoride glass fibers are operated in the astronomical K band (2.0-2.4 microm) in which their attenuation is low. We have measured very low differential chromatic dispersion, and the wideband fringe visibility is 0.9 if the two fiber arms have the same temperature. The thermal sensitivity of fibers with respect to their interferometric properties has been studied. The differential chromatic dispersion of the fibers is highly sensitive to the temperature difference. On the contrary, the coherent loss due to mismatch of polarization states is not significantly dependent on the temperature difference. Compensation of thermally induced differential dispersion by use of CaF2 glass plates is demonstrated.

Test of photonic crystal fiber in broadband interferometry.

Photonic crystal fibers (PCFs) are microstructured waveguides that are used in metrology, nonlinear optics, and coherent tomography. PCF studies are focused mainly on the improvement of dispersion properties and wide spectral single-mode operating domains. Consequently, in the astronomical context this kind of fiber is a good candidate for use in the design of a fiber-linked version of a stellar interferometer for aperture synthesis. We discuss the potential of these fibers to take advantage of wide spectral single-mode operation. We propose an experimental setup that acts as a two-beam interferometer that uses PCFs to measure fringe contrast at four wavelengths (670, 980, 1328, and 1543 nm), which correspond to the R, I, J, and H astronomical bands, respectively, with the same couple of PCFs. For this purpose we use, for the first time to our knowledge, a piezoelectric PCF optical path modulator.