FOXP3 gene promoter methylation in endometrial cancer cells.

AIM: To determine the methylation level of promoter region of the FOXP3 gene promoter depending on the heterogeneity of intracellular localization of its protein product in endometrial cancer (EC) cells and assess its relation to the clinical and morphological features of tumor. MATERIALS AND METHODS: Samples of surgical material of 40 EC patients who have not received any specific treatment before the surgery, were studied. Real time methylation-specific PCR (MSP) as well as morphological and immunohistochemical methods were used in the study. RESULTS: Methylation of promoter region of the FOXP3 gene was determined in all EC cases, but variability of the methylation level in EC cells from 45.0% to 85.0% was observed. With tumor progression and in tumors with deep (≥ 1/2) invasion in myometrium, an increase of the methylation level of the FOXP3 and of cell number with cytoplasmic FOXP3 localization was observed. In EC patients the correlation between of methylation level of the FOXP3 gene and the number of FOXP3(+) tumor cells with cytoplasmic expression (r = 0.41) was determined. CONCLUSION: The methylation level of FOXP3 gene promoter region and intracellular localization of its protein product are associated with tumor differentiation grade and the depth of myometrial invasion.

[The role of functional interhemispherical brain asymmetry in providing adaptation processes of organism to stress effects].

The adaptation of students with different lateral phenotype to stressful effects has been investigated. The results of the research testify the different sensitivity of persons with left-lateral and right-lateral phenotypes to the action of various stress factors. Persons with left-lateral phenotype are proved to be the least resistant to electrodermal and painful effects. As opposed to them the dextral manifested the most adequate adaptation to the given effects that was characterized by the least expressed physiological reactions.

[Changes in reactions in rats during hypokinesia and electromagnetic irradiation of ultrahigh frequency]. / Zminy reaktsiï orhanizmu shchuriv na hipokineziiu pry diï elektromahnitnoho vyprominiuvannia nadto vysokykh chastot.

The effects of low intensity electromagnetic fields (EMF) on the hypokinetic stress were studied on rats. It has been shown that exposure to EMF, combined with hypokinesia or without it, prevented the typical stress reaction on the limited motility. EMF was determined to modify the adaptive processes which resulted in the adaptive activation and limiting stress reaction. Adaptation was characterized with lower anxiety and excitability of the central nervous system, and a higher level of non-specific resistance, as compared to the stress reaction.