[Cell engineering and genetic approaches to the development of models of human embryonic stem cells for studying genetic disorders].

A novel approach to the establishment of genetically modified human embryonic stem cell (hESC) lines has been developed, and it has been shown that mutant hESC may be derived from affected embryos after preimplantation genetic diagnosis screening for a particular single gene disorder. Here we provide the description of embryo and cell manipulation procedures, diagnostic lay out, analysis of the efficiency of embryo development and hESC establishment, as well as developments for hESC derivation in animal free conditions. The high efficiency of the approach (50%) is especially crucial in the work with rare and unique resources, such as genetically screened embryos necessary for the derivation of hESC lines representative of specific genetic diseases.

[Bioelectrical activity and the ultrastructural characteristics of thyroid cell cytoplasmic membranes in different forms of thyrotoxicosis]. / Bioélektricheskaia aktivnost' i osobennosti ul'trastruktury tsitoplazmaticheskikh membran tireoidnykh kletok pri razlichnykh formakh tireotoksikoza.

Bioelectrical activity of the thyroid epithelium cells was studied by microelectrode analysis in patients with various forms of thyrotoxicosis, as well as in those with the disease developed against the background of organic affections of the central nervous system. The value of bioelectrical potentials of the thyroid cells in patients with various forms of thyrotoxicosis, including that coursing against the background of encephalopatheis, proved to be practically the same, and, at the same time, considerably lower than in control examinations. Cytoplasmic membranes of the thyroid cells retained, irrespective of the form and genesis of basedowification, the same structural organization, which, however, differed from that in intact thyrocytes.