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J Assist Reprod Genet ; 31(1): 25-33, 2014 Jan.
Article in English | MEDLINE | ID: mdl-24174299

ABSTRACT

PURPOSE: To determine the precision and accuracy of Accu-Beads and their utility as a quality control product for manual and automated measurements of sperm concentration. METHODS: This observational study was performed at an Assisted Reproductive Technology laboratory in a tertiary-care, university hospital. To simulate sperm concentration, bead concentrations were measured with the use of a manual and an automated method. RESULTS: The manual counts did not vary significantly from the automated counts regardless of the concentration. However, the counts did vary between lots of low concentration of Accu-Beads and between the two different types of fixed counting chambers. The two bead concentrations used in this study were below the 95 % confidence interval for the values listed by the manufacturer. CONCLUSION(S): In our laboratory, Accu-Beads met enough of the requirements of a good control material to be acceptable for daily quality control use, especially if we set our own ranges of acceptability for each vial of Accu-Beads. It is necessary to evaluate each new lot of Accu-Beads when they are received and again if they are used with a different counting chamber.


Subject(s)
Microspheres , Sperm Count/methods , Sperm Count/standards , Automation, Laboratory/standards , Cell Separation/methods , Cell Separation/standards , Humans , Male , Quality Control , Reproducibility of Results
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