ABSTRACT
This study investigates the mycelial biomass production and chitosan extraction potential of various Basidiomycota strains, including Heterobasidion annosum, Phanerochaete chrysosporium, Pleurotus ostreatus, Trametes versicolor, and Lentinus lepideus. Both submerged fermentation (SF) and solid-state fermentation (SSF) methods were employed. The chitosan yield in basidiocarps of Pleurotus ostreatus, Agaricus bisporus, and Ganoderma applanatum was also evaluated as a reference material. The chitosan extracted from fungal cells was characterized using elemental analyses and FTIR spectroscopy. Among the cultivated strains, P. chrysosporium exhibited the highest mycelial biomass concentration in SF (1.03 g 100 mL-1) after 14 days, while T. versicolor achieved the highest biomass concentration in SSF (3.65 g 100 mL-1). The highest chitosan yield was obtained from the mycelium of P. chrysosporium (0.38%) and T. versicolor (0.37%) in shaken SF. Additionally, commercially cultivated A. bisporus demonstrated the highest chitosan yield in fungal fruiting bodies (1.7%). The extracted chitosan holds potential as a functional biopolymer additive for eco-friendly materials, serving as an alternative to synthetic wet and dry strength agents in packaging materials.
ABSTRACT
To decrease climate changes, more research focuses on decreasing waste wood biomass (WWB) burning and increasing its conversion into value-added products. The WWB was isolated from model wood processing wastewater with a new hybrid coagulant by the coagulation/flocculation method. This study is aimed to characterize the WWB and to investigate its effect in the composition of a hybrid lignocellulosic filler on the properties of recycled polypropylene (rPP)-based wood-plastic composites (WPCs). The waste biomass contained high-molecular lignin and hemicelluloses substances and represented a finely dispersed powder. It was hydrophobic and was characterized by enhanced thermal stability. To minimize the negative effect of polymer wastes on the environment, recycled polypropylene as a polymer matrix was used with the hybrid filler in fabricating WPC samples. The presence of the coagulated WWB in the hybrid filler composition positively affects the mechanical properties, water uptake and dimensional stability of the composite samples. Such a behavior of the waste biomass showed its function as a compatibilizer, which promoted the interfacial adhesion in the composite system.
