Subject(s)
Immunosuppressive Agents , Streptomyces/metabolism , Animals , Carrier Proteins/metabolism , Cell Division/drug effects , Chemical Phenomena , Chemistry, Physical , Concanavalin A/pharmacology , DNA-Binding Proteins/metabolism , Heat-Shock Proteins/metabolism , Lipopolysaccharides/pharmacology , Magnetic Resonance Spectroscopy , Mice , Molecular Structure , Polyenes/chemistry , Polyenes/isolation & purification , Polyenes/metabolism , Polyenes/pharmacology , Pyrans/chemistry , Pyrans/isolation & purification , Pyrans/pharmacology , Recombinant Proteins/metabolism , Sirolimus , Spleen/cytology , Tacrolimus Binding ProteinsSubject(s)
Anti-Bacterial Agents/isolation & purification , Lactams , Streptomyces/analysis , beta-Lactams , Anti-Bacterial Agents/pharmacology , Chemical Phenomena , Chemistry , Chromatography, Paper/methods , Chromatography, Thin Layer/methods , Magnetic Resonance Spectroscopy , beta-Lactamase InhibitorsABSTRACT
The olivanic acids MM 4550, MM 13902 and MM 17880 are members of a new family of beta-lactam antibiotics. An isolation and purification process utilising ion-par extraction and ion-exchange chromatography is described and the metabolites are characterised by physico-chemical and biological properties.
Subject(s)
Anti-Bacterial Agents/isolation & purification , Streptomyces/metabolism , Anti-Bacterial Agents/biosynthesis , Anti-Bacterial Agents/pharmacology , Bacteria/drug effects , Chemical Phenomena , Chemistry, Physical , Fermentation , beta-Lactams/biosynthesis , beta-Lactams/isolation & purification , beta-Lactams/pharmacologyABSTRACT
Production of chlorflavonin, a new antifungal antibiotic, by strains of Aspergillus candidus is described. Two wild strains of the fungus had distinctly different chlorflavonin-producing capabilities. One strain produced 25 mug of chlorflavonin per ml per 4 to 5 days in a pilot scale fermentor with stirring, using a medium containing corn steep liquor and glucose. Production of antibiotic was favored by high rates of agitation-aeration. Crude chlorflavonin was extracted from the whole brew with a hydrocarbon solvent and then purified by recrystallization from benzene and petroleum ether. The overall yield from fermentation brew to pure product was 50%.