1.
Tsitologiia
; 28(5): 583-6, 1986 May.
Article
in Russian
| MEDLINE
| ID: mdl-3738992
ABSTRACT
A new flow-cytometric method is described that permits analysis of the number of chromosomes of individual cells. Preliminary stained mitotic cells are passed through a specially designed flow chamber in which they are destroyed just before passing through the focused laser beam of the flow cytometer. Signals from chromosomes of the destroyed cells are counted, and the results are represented as a distribution of the number of chromosomes in the cells. The method may be applied for the detection of relatively rare cells with abnormal numbers of chromosomes in biological and clinical research.
Subject(s)
Chromosomes/ultrastructure , Flow Cytometry/methods , Analog-Digital Conversion , Animals , Cells, Cultured , Cricetinae , Cricetulus , Equipment Design , Flow Cytometry/instrumentation , Lasers , Metaphase
2.
Dokl Akad Nauk SSSR
; 268(2): 458-61, 1983.
Article
in Russian
| MEDLINE
| ID: mdl-6832004