Issues in monitoring wilderness lake chemistry: A case study in the Sawtooth Mountains, Idaho.

A probability-based sample of 48 of the 152 lakes in the Sawtooth Wilderness, Idaho was conducted in summer 1988. Results from this sample were compared to the Environmental Protection Agency's 1985 probability survey of lakes throughout the western United States, which included 17 lakes in the Sawtooth Wilderness. Although methods differed in several respects, including year, season, sampling location within the lakes, holding times, and sample size, general characterization of lake chemistry for the population of lakes in the wilderness based on the two surveys was very similar. The results indicate that general lake characterization in difficult-to-access wilderness areas of the West can be achieved with a modest investment in resources with the use of volunteers. However, accurate measurement of some non-conservative and low-level analytes such as NO 3 (-) ,NH 4 (+) , total P, and aluminiun in the lakes probably requires more rigorous attention to sampling protocols and holding times. A two-stage sampling strategy employing extensive use of conductivity on a large number of lakes and intensive detailed chemical characterization on a smaller number of lakes offers an alternative design for describing large populations of wilderness lakes. The relatively high concentrations of fluoride and sulfate in many of the study lakes reflect the weathering of minerals not usually identified on geologic maps. These natural sources of acid anions violate the assumptions in commonly employed empirical models of acidification.

The feasibility of monitoring wilderness lake chemistry with remote sensing methods.

Wilderness lakes in the U.S. are at risk to the effects of atmospheric pollution. The results of the EPA Western Lake Survey, for example, show that many of the lakes in the West have very low concentrations of dissolved constituents, and are sensitive to acidification. Lakes located in wilderness areas were found to be particularly sensitive: median ANC values of 91.4 µeq/1 compared to 282.7 µeq/1 in non-wilderness areas. Sampling remote wilderness lakes is difficult and expensive. For example, the cost of sampling each lake in the National surface water survey was about $ 4000 per lake. Remote sensing methods may provide an alternative means of monitoring the chemistry of wilderness lakes. This study defines the feasibility of the remote monitoring of lake chemistry in the Adirondack Park region of New York by comparing measured lake chemistry with field reflectance measurements, remote reflectance measurements from aircraft and satellite and laboratory and airborne laser fluorosensor data.Water samples collected from a representative population of Adirondack Park lakes were analyzed for pH, acid neutralizing capacity (ANC), dissolved organic carbon (DOC), total reactive aluminum and plant pigment concentration concurrent with the collection of remote sensing data. Lake parameters estimated from field reflectance measurements which directly effect lake optical properties, total pigments, DOC, and turbidity were estimated with less error and more precision than those parameters not directly related to lake optics, pH, ANC and A1 concentration. Results from airborne MSS and Landsat MSS data produced lower R(2) values than estimates using field reflectance.The concentration of DOC can be estimated remotely by using laser fluorosensing. The spectral-fluorescence properties of DOC were correlated with the pH and aluminum concentrations in the lake water. Remotely measurable DOC fluorescence spectra were used to estimate DOC, H, and A1 concentrations.Of the methods investigated, laser fluorosensing shows the most promise for the remote prediction of lake DOC, hydrogen ion and aluminum concentration in Adirondack lakes while reflectance measures may be used to estimate lake chlorophyll, DOC and transparency. Given the large number of wilderness lakes and the difficulty of sampling in remote wilderness, remote sensing methods may provide an alternative, while less precise, method of monitoring lake chemistry. The applicability of these findings to lakes in other wilderness areas is unknown. Similar studies of the feasibility of monitoring Western wilderness lake chemistry with remote sensing methods are being initiated.

Water content and the conversion of phytochrome regulation of lettuce dormancy.

In an effort to determine which biological reactions can occur in relation to the water content of seeds, the regulation of lettuce seed dormancy by red and far red light was determined at various hydration levels. Far red light had an inhibiting effect on germination for seeds at all moisture contents from 4 to 32% water. Germination was progressively stimulated by red light as seed hydration increased from 8 to 15%, and reached a maximum at moisture contents above 18%. Red light was ineffective at moisture contents below 8%. Seeds that had been stimulated by red light and subsequently dried lost the enhanced germinability if stored at moisture contents above 8%. The contrast between the presumed photoconversion of phytochrome far red-absorbing (Pfr) to (Pr) occurring at any moisture content and the reverse reaction occurring only if the seed moisture content is greater than 8% may be explained on the basis of the existence of unstable intermediates in the Pr to Pfr conversion. Our results suggest that the initial photoreaction involved in phytochrome conversion is relatively independent of water content, while the subsequent partial reactions become increasingly facilitated as water content increases from 8 to 18%.