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1.
Int J Pharm ; 257(1-2): 273-81, 2003 May 12.
Article in English | MEDLINE | ID: mdl-12711182

ABSTRACT

In this study the possibility to deliver the acid-sensitive enzyme alkaline phosphatase (AP) from calf intestine (CIAP) to the intestinal system by oral administration was investigated. Tablets were prepared and in vitro evaluated. Final proof of concept studies were performed in rats. This acid labile enzyme is potentially useful in the treatment of sepsis, a serious condition during which endotoxins can migrate into the blood stream. The CIAP was freeze-dried with inulin and subsequently compacted into round biconvex tablets with a diameter of 4mm and a weight of 25-30 mg per tablet. The tablets were coated with an enteric coating in order to ensure their survival in the stomach. In vitro evaluation of tablets containing alkaline phosphatase from bovine intestine (BIAP) was the first step in the development. It was found that tablets without enteric coating dissolved rapidly in 0.10 M HCl with total loss of enzymatic activity of the alkaline phosphatase. Tablets that were coated were stable for at least 2 h in 0.10 M HCl, but dissolved rapidly when the pH was increased to 6.8. Furthermore, it was shown that the enzymatic activity of the released BIAP was fully preserved. The in vivo test clearly showed that the oral administration of enteric coated tablets resulted in the release of enzymatically active CIAP in the intestinal lumen of rats. The location of the enhanced enzymatic activity of AP in the intestines varied with the time that had passed between the administration of the tablets and the sacrificing of the rats. Also, the level of enzymatic activity increased with an increasing number of tablets that were administered.


Subject(s)
Alkaline Phosphatase/administration & dosage , Intestinal Mucosa/metabolism , Inulin/administration & dosage , Administration, Oral , Alkaline Phosphatase/chemistry , Animals , Dialysis , Enzyme Stability , Freeze Drying , Male , Rats , Rats, Wistar , Solubility , Tablets
2.
Vaccine ; 19(20-22): 2898-907, 2001 Apr 06.
Article in English | MEDLINE | ID: mdl-11282201

ABSTRACT

Local mucosal IgA antibodies play a central role in protection of the respiratory tract against influenza virus infection. Therefore, new-generation influenza vaccines should aim at stimulating not only systemic, but also local antibody responses. Previously, we demonstrated that the recombinant B subunit of the Escherichia coli heat-labile toxin (LTB) is a potent adjuvant towards nasally administered influenza subunit antigen. Here, we investigated the protection conferred by LTB-supplemented influenza subunit antigen given intranasally (i.n.) or intramuscularly (i.m.) to mice. Both i.n. and i.m. immunization with subunit antigen and LTB completely protected the animals against viral infection. Protection upon i.n. immunization was associated with the induction of antigen-specific serum IgG and mucosal IgA, whereas protection upon i.m. immunization correlated with strong serum and mucosal IgG, but not IgA responses. We conclude that LTB-supplemented influenza subunit antigen, given either i.n. or i.m, induces protective antibody-mediated mucosal immunity and thus represents a promising novel flu vaccine candidate.


Subject(s)
Adjuvants, Immunologic/administration & dosage , Antibodies, Viral/immunology , Bacterial Toxins/administration & dosage , Enterotoxins/administration & dosage , Escherichia coli Proteins , Immunoglobulin A, Secretory/immunology , Immunoglobulin G/immunology , Influenza Vaccines/immunology , Administration, Intranasal , Animals , Female , Immunization , Influenza Vaccines/administration & dosage , Injections, Intramuscular , Lung/virology , Mice , Mice, Inbred BALB C , Nasal Mucosa/virology , Protein Subunits , Rats
3.
Eur J Surg ; 166(12): 963-7, 2000 Dec.
Article in English | MEDLINE | ID: mdl-11152259

ABSTRACT

OBJECTIVE: To develop a standardised and reproducible model of intra-abdominal infection and abscess formation in rats. DESIGN: Experimental study. SETTING: University hospital, The Netherlands. SUBJECTS: 36 adult male Wistar rats. INTERVENTIONS: In 32 rats, peritonitis was produced using two different concentrations of Escherichia coli (E. coli) and Bacteroides fragilis (B. fragilis) incorporated in fibrin clots (E. coii 1 x 10(5) colony forming units (CFU)/ml or 1 x 10(8) CFU/ml, B. fragilis: 1 x 10(8) CFU/ml). Four rats with fibrin clots without bacteria served as uninfected controls. MAIN OUTCOME MEASUREMENTS: Macroscopy and bacterial counts in peritoneal fluid, blood, and fibrin clots after 24 hours, 4 days, 7 days, and 4 weeks. RESULTS: Macroscopically, there were signs of intra-abdominal infection and abscesses. With the higher starting concentration of E. coli, macroscopic signs were more pronounced and in nearly all rats bacterial counts in peritoneal fluid and fibrin clots showed persistently high numbers of E. coli and B. fragilis for at least 7 days (E. coli = 2 x 10(3) to 1 x 10(6) CFU/ml and 5 x 10(7) to 9 x 10(8) CFU/clot; B. fragilis = 1 x 10(3) to 1 x 10(6) CFU/ml and 5 x 10(7) to 6 x 10(8) CFU/clot). CONCLUSION: This standardised and reproducible model of intra-abdominal infection and abscess formation seems well suited for further use and development in experiments on the pathophysiology of intra-abdominal infection and abscesses.


Subject(s)
Abdominal Abscess , Disease Models, Animal , Peritonitis , Abdominal Abscess/microbiology , Animals , Bacteroides Infections , Bacteroides fragilis , Escherichia coli Infections , Male , Peritonitis/microbiology , Rats , Rats, Wistar
4.
Immunology ; 97(4): 706-13, 1999 Aug.
Article in English | MEDLINE | ID: mdl-10457227

ABSTRACT

The Escherichia coli heat-labile enterotoxin (LT) is an exceptionally effective mucosal immunogen and mucosal immunoadjuvant towards coadministered antigens. Although, in general, the molecular basis of these properties is poorly understood, both the toxic ADP-ribosylation activity of the LTA subunit and the cellular toxin receptor, ganglioside, GM1-binding properties of the LTB-pentamer have been suggested to be involved. In recent studies we found that GM1-binding is not essential for the adjuvanticity of LT, suggesting an important role for the LTA subunit in immune stimulation. We now describe the immunomodulatory properties of recombinant LTA molecules with or without ADP-ribosylation activity, LTA(His)10 and LTA-E112K(His)10, respectively. These molecules were expressed as fusion proteins with an N-terminal His-tag to allow simple purification on nickel-chelate columns. Their immunogenic and immunoadjuvant properties were assessed upon intranasal administration to mice, and antigen-specific serum immunoglobulin-isotype and -subtype responses and mucosal secretory immunoglobulin A (IgA) responses were monitored using enzyme-linked immunosorbent assay. With respect to immunogenicity, both LTA(His)10 and LTA-E112K(His)10 failed to induce antibody responses. On the other hand, immunization with both LT and the non-toxic LT-E112K mutant not only induced brisk LTB-specific, but also LTA-specific serum and mucosal antibody responses. Therefore, we conclude that linkage of LTA to the LTB pentamer is essential for the induction of LTA-specific responses. With respect to adjuvanticity, both LTA(His)10 and LTA-E112K(His)10 were found to stimulate serum and mucosal antibody responses towards coadministered influenza subunit antigen. Remarkably, responses obtained with LTA(His)10 were comparable in both magnitude and serum immunoglobulin isotype and subtype distributions to those observed after coimmunization with LT, LT-E112K, or recombinant LTB. We conclude that LTA, by itself, can act as a potent adjuvant for intranasally administered antigens in a fashion independent of ADP-ribosylation activity and association with the LTB pentamer.


Subject(s)
Antibodies, Bacterial/biosynthesis , Bacterial Toxins/immunology , Enterotoxins/immunology , Escherichia coli Proteins , Escherichia coli/immunology , Immunoglobulin A, Secretory/biosynthesis , Adjuvants, Immunologic , Administration, Intranasal , Animals , Antibodies, Viral/biosynthesis , Antigens, Viral/immunology , Immunity, Mucosal , Immunoglobulins/biosynthesis , Influenza B virus/immunology , Mice , Mice, Inbred BALB C , Recombinant Proteins/immunology
5.
Vaccine ; 16(20): 2069-76, 1998 Dec.
Article in English | MEDLINE | ID: mdl-9796066

ABSTRACT

The Escherichia coli heat-labile enterotoxin (LT) is a very potent mucosal immunogen. LT also has strong adjuvant activity towards coadministered unrelated antigens and is therefore of potential interest for development of mucosal vaccines. However, despite the great demand for such mucosal vaccines, the use of LT holotoxin as an adjuvant is essentially precluded by its toxicity. LT is composed of an A subunit, carrying the toxic ADP-ribosylation activity, and a pentamer of identical B subunits, which mediates binding to ganglioside GM1, the cellular receptor for the toxin. In this paper, we demonstrate that recombinant enzymatically inactive variants of LT, including the LTB pentamer by itself, retain the immunoadjuvant activity of LT holotoxin in a murine influenza model. Mice were immunized intranasally (i.n.) with influenza virus subunit antigen, consisting mostly of the isolated surface glycoprotein hemagglutinin (HA), supplemented with either recombinant LTB (rLTB), a nontoxic LT mutant (E112K, with a Glu112-->Lys substitution in the A subunit), or LT holotoxin, and the induction of systemic IgG and local S-IgA responses was evaluated by direct enzyme-linked immunosorbent assay (ELISA). Immunization with subunit antigen alone resulted in a poor systemic IgG response and no detectable S-IgA. However, supplementation of the antigen with E112K or rLTB resulted in a substantial stimulation of the serum IgG level and in induction of a strong S-IgA response in the nasal cavity. The adjuvant activity of E112K or rLTB under these conditions was essentially the same as that of the LT holotoxin. The present results demonstrate that nontoxic variants of LT, rLTB in particular, represent promising immunoadjuvants for potential application in an i.n. influenza virus subunit vaccine. Nontoxic LT variants may also be used in i.n. vaccine formulations directed against other mucosal pathogens. In this respect, it is of interest that LT(B)-stimulated antibody responses after i.n. immunization were also observed at distant mucosal sites, including the urogenital system. This, in principle, opens the possibility to develop i.n. vaccines against sexually transmitted infectious diseases.


Subject(s)
Antigens, Surface/immunology , Bacterial Toxins/immunology , Enterotoxins/immunology , Escherichia coli Proteins , Immunoglobulin A/biosynthesis , Immunoglobulin G/biosynthesis , Influenza Vaccines/immunology , Administration, Intranasal , Animals , Antigens, Surface/administration & dosage , Bacterial Toxins/administration & dosage , Bacterial Toxins/genetics , Enterotoxins/administration & dosage , Enterotoxins/genetics , Enzyme-Linked Immunosorbent Assay , Escherichia coli , Immunity, Mucosal , Influenza Vaccines/administration & dosage , Mice , Mutation
6.
Immunology ; 94(3): 424-30, 1998 Jul.
Article in English | MEDLINE | ID: mdl-9767427

ABSTRACT

Escherichia coli (E. coli) heat-labile toxin (LT) is a potent mucosal immunogen and immunoadjuvant towards co-administered antigens. LT is composed of one copy of the A subunit, which has ADP-ribosylation activity, and a homopentamer of B subunits, which has affinity for the toxin receptor, the ganglioside GM1. Both the ADP-ribosylation activity of LTA and GM1 binding of LTB have been proposed to be involved in immune stimulation. We investigated the roles of these activities in the immunogenicity of recombinant LT or LTB upon intranasal immunization of mice using LT/LTB mutants, lacking either ADP-ribosylation activity, GM1-binding affinity, or both. Likewise, the adjuvant properties of these LT/LTB variants towards influenza virus subunit antigen were investigated. With respect to the immunogenicity of LT and LTB, we found that GM1-binding activity is essential for effective induction of anti-LTB antibodies. On the other hand, an LT mutant lacking ADP-ribosylation activity retained the immunogenic properties of the native toxin, indicating that ADP ribosylation is not critically involved. Whereas adjuvanticity of LTB was found to be directly related to GM1-binding activity, adjuvanticity of LT was found to be independent of GM1-binding affinity. Moreover, a mutant lacking both GM1-binding and ADP-ribosylation activity, also retained adjuvanticity. These results demonstrate that neither ADP-ribosylation activity nor GM1 binding are essential for adjuvanticity of LT, and suggest an ADP-ribosylation-independent adjuvant effect of the A subunit.


Subject(s)
Adjuvants, Immunologic , Bacterial Toxins/immunology , Enterotoxins/immunology , Escherichia coli Proteins , Escherichia coli , G(M1) Ganglioside/physiology , Immunity, Mucosal/physiology , Adenosine Diphosphate Ribose/metabolism , Administration, Intranasal , Animals , Antigens, Viral/administration & dosage , Bacterial Toxins/genetics , Enterotoxins/genetics , Female , G(M1) Ganglioside/metabolism , Immunization , Influenza A virus/immunology , Mice , Mice, Inbred BALB C , Mutagenesis, Site-Directed , Recombinant Proteins/immunology
7.
Eur J Immunol ; 28(4): 1243-50, 1998 Apr.
Article in English | MEDLINE | ID: mdl-9565364

ABSTRACT

The Escherichia coli heat-labile enterotoxin (LT) is known for its potent mucosal immunoadjuvant activity towards co-administered antigens. LT is composed of one A subunit, which has ADP-ribosylation activity, and a homopentameric B subunit, which has high affinity for the toxin receptor, ganglioside GM1. In previous studies, we have investigated the role of the LTA and LTB subunits in the adjuvanticity of LT towards influenza virus hemagglutinin (HA), administered intranasally to mice. We now studied the adjuvant properties of LT and LT variants towards keyhole limpet hemocyanin (KLH), which, in contrast to HA, does not bind specifically to mucosal surfaces. It is demonstrated that LT mutants without ADP-ribosylation activity, as well as LTB, retain mucosal immunoadjuvant activity when administered intranasally to mice in conjunction with KLH. As with influenza HA, adjuvanticity of LTB required GM1-binding activity, whereas GM1-binding was not essential for adjuvant activity of LT. Furthermore, we found that also recombinant LTA alone acts as a potent mucosal adjuvant, and that this adjuvanticity is independent of ADP-ribosylation activity. It is concluded that binding of the antigen to mucosal surfaces does not play an essential role in the immunostimulation by LT and LT variants, and that both recombinant LTA and LTB represent powerful nontoxic mucosal adjuvants.


Subject(s)
Adenosine Diphosphate Ribose/immunology , Adjuvants, Immunologic/administration & dosage , Bacterial Toxins/immunology , Enterotoxins/immunology , Escherichia coli Proteins , Hemocyanins/immunology , Adenosine Diphosphate Ribose/genetics , Administration, Intranasal , Animals , Bacterial Toxins/genetics , Bacterial Toxins/metabolism , Binding Sites/genetics , DNA Mutational Analysis , Enterotoxins/genetics , Enterotoxins/metabolism , Escherichia coli , Female , GTP-Binding Proteins/metabolism , Hemocyanins/administration & dosage , Mice , Mice, Inbred BALB C
8.
Infect Immun ; 64(12): 5413-6, 1996 Dec.
Article in English | MEDLINE | ID: mdl-8945598

ABSTRACT

The Escherichia coli heat-labile enterotoxin (LT) is a potent inducer of mucosal immune responses. In a previous study (L. DeHaan, W. R. Verweij, M. Holtrop, E. Agsteribbe, and J. Wilschut, Vaccine 14:620-626, 1996), we have shown that efficient induction of an LTB-specific mucosal immune response by LT requires the presence of the LTA chain, suggesting a possible role of the enzymatic activity of LTA in the induction of these responses. In the present study, we generated LT mutants with altered ADP-ribosylation activities and evaluated their immunogenicity upon intranasal administration to mice. The results demonstrate that the mucosal immunogenicity of LT is not dependent on its ADP-ribosylation activity.


Subject(s)
Enterotoxins/genetics , Escherichia coli/genetics , Immunity, Mucosal , Adenosine Diphosphate/genetics , Adenosine Diphosphate/immunology , Animals , Enterotoxins/immunology , Escherichia coli/immunology , Hot Temperature , Mice , Mutation
9.
Vaccine ; 14(4): 260-6, 1996 Mar.
Article in English | MEDLINE | ID: mdl-8744549

ABSTRACT

The Escherichia coli heat-labile enterotoxin (LT) is a potent mucosal immunogen, inducing high secretory as well as systemic antibody responses upon oral or intranasal (i.n.) administration. In addition, LT has the capacity to act as an adjuvant in antibody responses against coadministered other antigens. To investigate the role of the individual subunits of LT in the mucosal immunogenicity and adjuvanticity of LT, the LT holotoxin and the non-toxic B subunit (LTB) were cloned separately and purified from overproducing E. coli cultures. Mice were immunized i.n. with the recombinant LT, LTB and combinations of the two and the induction of LTB-specific serum IgG and IgA as well as mucosal S-IgA was monitored. Intranasal administration of 2 micrograms LTB by itself induced a moderate systemic and a low mucosal antibody response, the latter being restricted to the site of immunization. However, addition of a trace amount (50 ng) of LT holotoxin to LTB strongly stimulated both serum antibody and mucosal S-IgA responses to LTB: the antibody levels induced by 2 micrograms LTB supplemented with 50 ng LT were similar to those seen after immunization with 2.9 micrograms of the LT holotoxin alone (representing an amount of 2 micrograms LTB). Furthermore, immunization with LT-supplemented LTB or with LT holotoxin alone, but not immunization with LTB alone, induced an S-IgA response in distant mucosal tissues including the lung, intestine and the urogenital system. Nicking of the LTA chain with trypsin did not enhance the immunogenicity of LT. These results indicate that, although the LTA chain plays an important role in the mucosal immunogenicity of LT including priming of the common mucosal immune system, extremely low amounts of the LT holotoxin suffice for the induction of high antibody responses to LTB, the trace LT and LTB acting in a synergistic fashion.


Subject(s)
Bacterial Toxins/immunology , Enterotoxins/immunology , Escherichia coli Proteins , Immunity, Mucosal/immunology , Administration, Intranasal , Animals , Antibodies, Bacterial/blood , Antibody Formation/drug effects , Bacterial Toxins/biosynthesis , Bacterial Toxins/pharmacology , Enterotoxins/biosynthesis , Enterotoxins/pharmacology , Female , Immunity, Mucosal/drug effects , Immunoglobulin A, Secretory/immunology , Immunoglobulin G/biosynthesis , Immunoglobulin G/blood , Lung/metabolism , Macromolecular Substances , Mice , Mice, Inbred BALB C , Nasal Mucosa/metabolism , Recombinant Proteins/biosynthesis , Recombinant Proteins/immunology , Recombinant Proteins/pharmacology , Swine
10.
J Gen Microbiol ; 139(8): 1739-44, 1993 Aug.
Article in English | MEDLINE | ID: mdl-8409916

ABSTRACT

A fibrin clot model for intra-abdominal abscess formation was used to study the migratory properties of peritoneal cells from rats during the early stages of infection. Peritoneal cells and fibrin clot remnant were harvested 6 h after implantation of a sterile, singly infected (Escherichia coli or Bacteroides fragilis) or mixed infected (E. coli and B. fragilis) fibrin clot. Histological study of fibrin clots, removed 6 h after implantation, showed a deeper infiltration by host cells of B. fragilis infected clots compared to the others. This difference in infiltration by peritoneal cells was not due to differences in fibrinolytic activity of the bacterial strains. Differential cell counts of the peritoneal cells from rats implanted with sterile, singly and mixed infected fibrin clots showed distribution over subpopulations to be independent of the bacterial content of the infected clots used. In vitro migration assays showed no significant differences in migration by peritoneal cells from rats implanted with clots containing a different bacterial composition. Since B. fragilis infected fibrin clots were more deeply infiltrated by host defence cells than the other clots, and only mixed infected clots led to persistent abscesses in this model, we conclude that local conditions within the fibrin matrix rather than intrinsic cellular capacities of the host cells are important for the process of abscess formation.


Subject(s)
Bacteroides Infections/pathology , Escherichia coli Infections/pathology , Peritoneal Cavity/pathology , Animals , Bacterial Capsules/metabolism , Bacteroides fragilis , Cell Movement , Cells, Cultured , Fibrin/metabolism , Fibrinolysis , Humans , Male , Rats , Rats, Wistar
11.
J Med Microbiol ; 38(1): 13-8, 1993 Jan.
Article in English | MEDLINE | ID: mdl-8380297

ABSTRACT

Peritoneal cells from rats infected intraperitoneally with Escherichia coli and Bacteroides fragilis, alone or in combination were examined in vitro. Cells were harvested 6 h after implantation of fibrin clots infected with E. coli or B. fragilis, separately or containing both species, and assayed for their bactericidal capacities, chemiluminescence and production of cidal metabolites. Peritoneal cell populations from rats with implants of any of the infected clots showed similar distribution of different subpopulations. Bactericidal activity of peritoneal cells did not differ with the bacterial species used. Chemiluminescence values of peritoneal cells from rats with mono-infected B. fragilis or mixed-infected implanted clots, after stimulation with either particles or chemical stimuli, were significantly higher than those of rats with mono-infected E. coli or sterile clots. The same tendency was seen with regard to the production of cidal metabolites such as hydrogen peroxide and superoxide anions although no significant differences were found.


Subject(s)
Bacteroides Infections/immunology , Bacteroides fragilis/immunology , Escherichia coli Infections/immunology , Escherichia coli/immunology , Peritoneal Cavity/cytology , Animals , Bacteroides Infections/complications , Cells, Cultured , Colony Count, Microbial , Eosinophils/immunology , Escherichia coli Infections/complications , Hydrogen Peroxide/metabolism , Luminescent Measurements , Macrophages/immunology , Male , Neutrophils/immunology , Rats , Rats, Wistar , Superoxides/metabolism
12.
Infect Immun ; 59(9): 2999-3003, 1991 Sep.
Article in English | MEDLINE | ID: mdl-1879923

ABSTRACT

Under conditions of iron starvation, Bacteroides fragilis expresses various iron-repressible outer membrane proteins (IROMPs). A 44-kDa protein appears to be one of the major outer membrane proteins (OMPs) in B. fragilis under iron stress and plays a role in heme uptake by this bacterium. To determine whether the 44-kDa IROMP of B. fragilis is expressed in vivo and whether this protein is immunogenic, we used Western immunoblotting to examine serum samples from patients with an infection caused by Bacteroides species. All the serum samples from patients and from normal controls showed reactivity with several proteins of B. fragilis. Only serum samples from patients infected with B. fragilis showed immunoreactivity with the 44-kDa protein. We also used a rat infection model to study the immune response against this protein during the process of an intra-abdominal infection in these animals. During the first 8 days of infection a gradual increase of antibodies to the 44-kDa protein in the rat was detected. These results suggest that the 44-kDa IROMP is expressed in vivo, since it induces an antibody response in patients and animals. We also analyzed 85 strains of the B. fragilis group for the presence of proteins antigenically related to the B. fragilis 44-kDa protein. The data indicate that this protein was conserved in B. fragilis strains and was absent in the other bacterial strains tested.


Subject(s)
Bacterial Outer Membrane Proteins/metabolism , Bacteroides Infections/immunology , Bacteroides fragilis/metabolism , Iron/metabolism , Animals , Antibodies, Bacterial/analysis , Bacteroides fragilis/growth & development , Bacteroides fragilis/immunology , Blotting, Western , Electrophoresis, Polyacrylamide Gel , Humans , Immunoblotting , Molecular Weight , Rats
13.
J Med Microbiol ; 35(1): 18-22, 1991 Jul.
Article in English | MEDLINE | ID: mdl-2072375

ABSTRACT

Growth of Bacteroides fragilis and Escherichia coli was monitored during early stages of single (mono-) and mixed intra-abdominal infection in a rat fibrin clot model. When B. fragilis and E. coli were together involved in the infection, B. fragilis numbers increased about 6 h after an initial decline. This increase was not found with B. fragilis mono-infections. The numbers of E. coli increased rapidly in both mono- and mixed infections and stayed high for several days, but only mixed infection resulted in abscesses that persisted for more than 7 days. Macrophages, the main component of the peritoneal cellular defence mechanism, were outnumbered by polymorphonuclear leucocytes during the first 6 h of infection. Further characterisation of the macrophage population by means of monoclonal antibodies showed a shift from resident to exudate macrophages as the result of influx of the latter.


Subject(s)
Bacteroides Infections/complications , Escherichia coli Infections/complications , Abdomen , Animals , Bacteroides Infections/pathology , Bacteroides fragilis/growth & development , Blood Coagulation , Cell Count , Colony Count, Microbial , Disease Models, Animal , Escherichia coli/growth & development , Escherichia coli Infections/pathology , Fibrin , Humans , Male , Peritoneal Cavity , Rats , Rats, Inbred Strains
14.
Curr Genet ; 11(1): 25-34, 1986.
Article in English | MEDLINE | ID: mdl-2834078

ABSTRACT

We have used a variety of methods to localize the genes for ribosomal proteins S7 and S12 on Spirodela chloroplast DNA. Heterologous hybridization with a rps12 gene specific probe from Euglena has revealed the presence of rps12 homologous sequences within the inverted repeat of Spirodela chloroplast DNA on the fragment BamHI-V. In the partial nucleotide sequence of this fragment, two regions of amino acid sequence homology to Euglena S12 can be identified, separated from each other by a 542 bp intron with conserved boundary sequences. As was found for Nicotiana S12, the Spirodela S12 coding regions are for 85 amino acids homologous (79%) to E. coli S12 (starting from residue 38 to the C-terminus). Likewise, we are unable to identify the 37 5' terminal codons of rps12 in Spirodela. The functionality of the Spirodela rps12 sequence is discussed. The rps7 gene is located adjacent to rps12. Chloroplast ribosomal protein C-S11 (homologous to S7) has been detected by immunoprecipitation with both a polyspecific anti 30S serum and an anti C-S11 serum, among the in vitro translation products of mRNAs selected by Spirodela chloroplast DNA fragments BamHI-V and BamHI-P. Since in a DNA dependent E. coli cell free system, only BamHI-V appears to be capable of synthesis of C-S11, it is concluded that rps7 is located entirely within BamHI-V and is transcribed into a mRNA which extends into BamHI-P. As determined by Northern hybridization experiments, rps7 is cotranscribed with rps12; a stable transcript of approx. 1100 b is detected in total cellular Spirodela RNA with either rps12 and rps7 gene specific probes. The rps12 probe additionally detects an approx. 600 b transcript, which presumably corresponds to the excised rps12 intron RNA. Finally we have examined the expression of both rps7 and rps12 during light induced chloroplast development by Northern blotting and by immunoblotting. It is shown, that the steady-state levels of neither chloroplast ribosomal protein transcripts, nor those of the chloroplast ribosomal proteins itself, change significantly during the greening process.


Subject(s)
Chloroplasts/metabolism , DNA/genetics , Genes , Plants/genetics , Ribosomal Proteins/genetics , Amino Acid Sequence , Base Sequence , DNA Restriction Enzymes , Molecular Sequence Data , Repetitive Sequences, Nucleic Acid
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