ABSTRACT
This work was devoted to study of the peculiarities of morphology of the retina with ganglion cells complex and the peculiarities of regional and systemic blood circulation in surgeons and therapists. It was revealed that longtime work under operative microscope results in a decrease of all parameters of blood circulation, that with the data of optical coherent tomography of the retina and optic nerve in surgeons demonstrate the signs of the existence of more high risk of vascular eye pathology then that observed in therapists.
Subject(s)
Eye/pathology , Occupational Diseases/pathology , Optic Nerve/pathology , Physicians , Surgical Procedures, Operative , Adult , Eye/blood supply , Female , Humans , Male , Middle Aged , Occupational Diseases/prevention & control , Optic Nerve/blood supply , Risk FactorsABSTRACT
On isolated retinal preparation from STZ-induced diabetic rats in whole-cell configuration the basic electrical properties of retinal ganglion cells (RGC) were studied. It was shown that on 8th week after STZ maximal depolarization-induced firing frequency is reduced and decay time constant of high-frequency stimulation-induced calcium signals is increased as compared to controls. The data obtained suggest functional changes in RGC of STZ-induced diabetic rats, possible mechanisms of such changes are discussed.
Subject(s)
Diabetes Mellitus, Experimental/physiopathology , Membrane Potentials/physiology , Retinal Ganglion Cells/physiology , Streptozocin/pharmacology , Animals , Calcium Signaling/physiology , Cell Membrane/drug effects , Cell Membrane/metabolism , Cell Membrane/physiology , Diabetes Mellitus, Experimental/metabolism , Electric Stimulation , In Vitro Techniques , Membrane Potentials/drug effects , Patch-Clamp Techniques , Rats , Retinal Ganglion Cells/drug effectsABSTRACT
Ionic currents responsible for excitability of retinal ganglion cells (RGC) freshly isolated from eyes of adult rats were studied. Kutward potassium currents in RGC represented by populations of slow inactivating and non-inactivating currents. Inward sodium currents were completely blocked by extracellular application of 0.25 mM TTX. Calcium conductance in freshly isolated RGC has two components: low- and high-threshold. Extracellular application of nifedipine (10 mM) or Cd2+ (10 mM) blocked high threshold calcium currents by 28% and 60%, correspondingly, without any significant effect on low threshold calcium currents.
