GE132+Natural: Novel promising dietetic supplement with antiproliferative influence on prostate, colon, and breast cancer cells.

PURPOSE: Natural products have been investigated for promising new leads in pharmaceutical development. The purpose of this study was to analyze the biological effect of GE132+Natural, a novel supplement consisting of 5 compounds: Resveratrol, Ganoderma lucidum, Sulforaphane, Lycopene and Royal jelly. METHODS: The antiproliferative activity of GE132+Natural was tested on 3 different human cancer cell lines: MCF7 (breast cancer cells), PC3 (prostate cancer cells), and SW480 (colon cancer cells), as well as on EA.hy 926 (normal human endothelial cell line). In addition, the cytotoxicity of GE132+- Natural on the proliferation of primary human mesenchymal stem cells isolated from dental pulp (DP=MSC), along with its in vitro impact on different peripheral blood parameters, was determined. RESULTS: The results revealed high antiproliferative activity of GE132+Natural on all tested cancer cell lines (PC3, MCF7 and SW480), as well as on the EA.hy 926 endothelial cell line in a dose-dependent manner. However, applied in a wide range of concentrations GE132+Natural did not affect both the proliferation of primary mesenchymal stem cells and the peripheral blood cells counts. CONCLUSION: The data obtained demonstrated that GE132+Natural is effective in inhibiting cancer cell proliferation, indicating its potential beneficial health effects. In addition, the results pointed that adult mesenchymal stem cells might be valuable as a test system for evaluating the toxicity and efficacy of new medicines or chemicals.

Strenuous exercise and action of antioxidant enzymes.

BACKGROUND: During the course of evolution, the antioxidant system has developed in order to prevent cell damage and to maintain equilibrium between production of free radicals and their neutralization. Because of increased oxygen consumption and energy utilization during physical work, oxidative stress may be expected. A review of current literature revealed no consistent data regarding activity of antioxidant enzymes during strenuous exercise. OBJECTIVES: The aim of this paper was to identify the activity of antioxidant enzymes in erythrocytes during strenuous short-term exercise. METHODS: The activity of superoxide dismutase (SOD), glutathione peroxidase (GPX), and glutathione reductase (GR) were investigated. Also, glucose-6-phosphate dehydrogenase (G-6-PDH) activity was studied in order to assess the level of recovery of reduced glutathione (GSH) by maintaining NADPH. Peripheral blood was drawn before (1) and immediately after (2) the test completed. Modified Astrand's protocol on a bicycle ergometer was used to achieve strenuous exercise in volunteers. RESULTS: The results obtained were: SOD1--897.95 +/- 99.85 U/g Hb and SOD2--828.38 +/- 75.12 U/g Hb; GPX1--30.19 +/- 4.03 U/g Hb and GPX2--26.00 +/- 4.85 U/g Hb; GR1--58.29 +/- 8.23 U/L and GR2--62.67 +/- 7.29 U/L; G-6-PDH1--14.97 +/- 3.79 IU/g Hb and G-6-PDH2--16.5 +/- 2.74 IU/g Hb. The difference was statistically highly significant: p < 0.001 for SOD, GPX, and GR activities, and p = 0.018 for G-6-PDH activity. CONCLUSION: The results reflect the presence of oxidative stress in erythrocytes after strenuous exercise. Further investigations should clarify the role of other antioxidant-relevant compounds during exercise such vitamins, trace elements, and metals and enable estimation of the level of damage caused by generation of free radicals (e.g., malonyl dialdehyde, 8-hydroxydeoxyguanosine etc.). All this information together will undoubtedly give us clear insight into free radical generation during strenuous workloads.

Comparison of certain biochemical changes during exercise tests on treadmill and bicycle-ergometer with equal workload intensity.

Ergometrics, using both treadmill (T) and bicycle-ergometer (B), plays an important role in assessing physical work capacity. In addition to the other important parameters (blood pressure, heart rate, oxygen consumption, oxygen and carbon dioxyde-partial pressure in blood, etc.), it is of interest to follow, measure and compare values of certain metabolic products immediately after completion of exercise on T and B. The aim of this study was to define which test develops the most significant changes in metabolic parameters when the same workload intensity is maintained. The metabolic parameters measured were: lactate, pyruvate, 2.3-diphosphoglycerate (2.3-DPG), lactate dehydrogenase (LDH) and aldolase activity. The mean values obtained at the end of both tests were: lactate 3.17 mmol/l +/- 1.017 mmol/l (T) and 4.28 mmol/l +/- 1.2 mmol/l (B); pyruvate 78.17 mumol/l +/- 30.32 mumol/l (T) and 92.16 mumol/l +/- 32.73 mumol/l (B); 2.3-DPG 3.58 mmol/l +/- 1.31 mmol/l (T) and 3.93 mmol/l +/- 1.44 mmol/l (B); LDH activity 239.22 U/I +/- 38.24 U/I (T) and 260.5 U/I +/- 61.27 U/I (B); aldolase activity 4.93 U/I +/- 1.45 U/I (T) and 5.88 U/I +/- 2.31 U/I (B). The results obtained indicate that a much higher production and/or lower elimination of acid metabolic products occurs during the test on B. This is most likely due to increased muscle pressure in big muscle groups in the legs which, by compressing the local blood vessels, diminish oxygen supply and the elimination of acid metabolic products. According to the results of the study, the use of T can be recommended as a more appropriate method for physical work capacity assessment of untrained individuals in occupational medicine.