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Vet Parasitol ; 178(3-4): 293-9, 2011 Jun 10.
Article in English | MEDLINE | ID: mdl-21324600

ABSTRACT

Anisakids from 5 different species of cetacean, Kogia breviceps, Peponocephala electra, Stenella clymene, Stenella longirostris and Steno bredanensis, were submitted to genetic analysis. Adults and larvae fixed in ethanol-formalin-acetic acid or in 70% ethanol for periods ranging from 10 months to 10 years were isolated from 9 cetaceans stranded on Ceará coast, Northeast Brazil. The 18S rDNA gene, ITS1, and specific Anisakis typica ITS regions were amplified by PCR. 18S rDNA and ITS1 region confirmed Anisakis sp. morphological identification but also detected the presence of Aspergillus sp. in longer preserved samples. All samples were identified as A. typica by ITS species-specific PCR. The study report three new definitive hosts of A. typica from the Brazilian Atlantic coast by genetic analysis: P. electra, K. breviceps, and S. clymene.


Subject(s)
Anisakiasis/veterinary , Anisakis/isolation & purification , Cetacea/parasitology , Genetic Variation/genetics , Polymorphism, Single Nucleotide/genetics , Animals , Anisakiasis/parasitology , Anisakis/anatomy & histology , Anisakis/genetics , Base Sequence , Brazil , DNA, Helminth/chemistry , DNA, Helminth/genetics , DNA, Ribosomal Spacer/chemistry , DNA, Ribosomal Spacer/genetics , Molecular Sequence Data , Phylogeny , Polymerase Chain Reaction/veterinary , RNA, Ribosomal, 5.8S/chemistry , RNA, Ribosomal, 5.8S/genetics , Sequence Alignment
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