ABSTRACT
Kidney (n = 297), liver (n = 52), jawbone (n = 80) and muscle (n = 48) samples collected from red deer (Cervus elaphus) from north-eastern Croatia in the 2002--05 hunting season were analysed for cadmium (Cd), copper (Cu), iron (Fe), mercury (Hg), lead (Pb), selenium (Se) and zinc (Zn) using atomic absorption spectrometry. Statistical evaluation of results showed age-related accumulations of renal cortex Cd and Zn, bone Pb, and muscle Zn. Renal cortex Cd and Zn were significantly associated. In addition, concentrations of Cd and Pb in muscle tissue were significantly correlated with Fe content. Found levels of toxic metals were not likely to affect the health status of animals. A total of 49% of the muscle, 60% of the kidney and 6% of the liver samples were unsuitable for human consumption according to Croatian regulations for Cd in food. However, the calculated intake of Cd through deer meat consumption is small and represents no health risk when consumption is moderate.
Subject(s)
Deer , Environmental Pollutants/analysis , Food Contamination/analysis , Metals/analysis , Animals , Cadmium/analysis , Copper/analysis , Croatia , Environmental Monitoring/methods , Iron/analysis , Jaw/chemistry , Kidney/chemistry , Lead/analysis , Liver/chemistry , Mercury/analysis , Muscle, Skeletal/chemistry , Selenium/analysis , Spectrophotometry, Atomic , Zinc/analysisABSTRACT
SETTING: This paper describes an outbreak of human and related bovine tuberculosis (TB) caused by Mycobacterium caprae in Croatia. A 13-year-old boy clinically presented enlargement of cervical lymph node with consecutive isolation of M. caprae. His 7-year-old sister, who had no clinical signs of disease, hyper-reacted to the purified protein derivative (PPD) test (>25 mm) and peribronchial infiltration was found by radiology. The children came from a family that ran a small-sized cattle dairy farm. DESIGN: All cattle on the farm were subjected to cutaneous TB testing: six of the 14 reacted positive, while three were suspicious. The entire herd was slaughtered, their carcasses examined and collected material subjected to pertinent diagnostic procedures. RESULTS: Gross examination findings consistent with TB were observed in the PPD-positive cows. Mycobacteria isolated from the boy and cattle were identified by classical and molecular methods, confirming M. caprae as the causative agent. CONCLUSION: Although not bacteriologically proven, consumption of raw milk or non-pasteurised milk products from infected dairy cattle was suspected as the source of infection in humans. Our findings confirm the domination of M. caprae among cattle in Croatia and represent the first evidence of M. caprae infection in humans in Croatia.
Subject(s)
DNA, Bacterial/analysis , Dairying , Mycobacterium bovis/isolation & purification , Tuberculosis, Bovine/diagnosis , Tuberculosis/diagnosis , Zoonoses , Adolescent , Adult , Animals , Antitubercular Agents/therapeutic use , Bacterial Typing Techniques/veterinary , Cattle , Croatia , Female , Humans , Male , Milk/microbiology , Mycobacterium bovis/classification , Mycobacterium bovis/genetics , Treatment Outcome , Tuberculin Test/veterinary , Tuberculosis/drug therapy , Tuberculosis/genetics , Tuberculosis/microbiology , Tuberculosis/transmission , Tuberculosis, Bovine/genetics , Tuberculosis, Bovine/pathology , Tuberculosis, Bovine/transmissionABSTRACT
Three synthetic routes to salts of 5-amino-5-hydroxy-2,4,6(1H,3H,5H)-pyrimidinetrione (10) are described. The key reactions involved acid-catalyzed cleavage of 5-amino-5-ureido-2,4,6(1H,3H,5H)-pyrimidinetrione (7), conversion of uramil (8) to dehydrouramil (9) and subsequent hydration, and the condensation of alloxan (5) with ammonium salts. The carbinol ammonium salt structure 10a was unambiguously established by X-ray crystallography. New alloxan-like compounds 7, 9, and 10 were evaluated for diabetogenic activity in rats. Compound 7 was inactive, whereas compounds 9 and 10 showed the highest activity comparable to that of streptozotocin (12).
