ABSTRACT
In vitro analysis of anticoagulant compounds with a potential use as antithrombotic drugs, has been traditionally performed using techniques like spectrophotometry, turbidimetry, as well as electrochemical and clinical assays. Although, these techniques have some disadvantages such as: the inability to measure the total biological activity of thrombin, interferences and, sometimes, the quantitative determination of the inhibition ratio is not accurate. In the present work, the conversion of fibrinogen to fibrin was monitored by molecular exclusion chromatography (SEC-HPLC) in three different reaction systems. An inhibition percentage of 43.19±2.02% was obtained using heparin as an anticoagulant, in addition to the determination of the percentage of heparin bonded to thrombin. This methodology has not been previously described and has high potential for the determination of anticoagulant capacity with higher precision, the determination of thrombin's total biological activity and the quantitative determination of the inhibition ratio.
Subject(s)
Chromatography, Gel/methods , Chromatography, High Pressure Liquid/methods , Fibrinogen/metabolism , Thrombin/antagonists & inhibitors , Fibrin/analysis , Fibrin/metabolism , Fibrinogen/analysis , Heparin/pharmacology , Humans , Thrombin/analysis , Thrombin/metabolismABSTRACT
El síndrome hepatopulmonar es una entidad clínica que compromete seseramente el estado funcional de los pacientes con enfermedad hepática en estado terminal. No se puede predecir con exactitud la historia natural de este esndrome una veZ ha sido diagnosticado. Sin embargo se reconoce que queronóstico es grave, lentamente progresivo y aunque la causa de muerte suele estar relacionada a la enfermedad depática, la calidad de vida de estos pacientes se se notablemente alterada por el trastornoventilatorio asoccado. Se considera que el trasplante hepático, en casos bien seleccionados, puede logralla completa resolución de este síndrome y el éxito en elmanejo perioperatorioestá directamente relacionado con el cococimiento de la fisiopatología.
Hepatopulmonary syndrome is a clinical entiy that severely impairs functional status of patients with end stage liver disease. Natural lourse of this syndrome is unpredictable once ii has beeeniagnosed. Hotuevev it is known that its prognosisis seúous, slowly progressive and although cause of death is often related to liver disease, quality of Ufe in these patients ii significantly impaired by yhe additional ventilatory disorder. It is actually considered that liver transplantation, in selected cases, can lead to complete tesolution on this syndrome and so soccess in the peúoperative management is tisectly related to the knotuledge of the pathophysiology process.
Subject(s)
HumansABSTRACT
Se consultó nuestra base de datos institucional en búsqueda de pacientes menores de 10 kg. llevados a trasplante hepático ortotópico (THO) en un periodo de 10 años. Se registraron las variables demográficas y clínicas preoperatorias relevantes y el estado de severidad de la enfermedad al ingreso mediante la escala para enfermedad hepática terminal en pacientes pediátricos (PELD). Cada paciente fue seguido por 3 meses o hasta su deceso. Una vez estratificados los datos por tipo de donante y año de procedimiento, se analizaron las variables perioperatorias que tuvieran una asociación significativa con mortalidad postoperatoria temprana mediante el análisis univariado y regresión logística. Se consideraron significativos valores de p<0.05.
Subject(s)
Child , Infant Mortality , Liver Transplantation/adverse effects , Liver Transplantation/mortalityABSTRACT
This work presents the results concerning the first use of activated composite membranes (ACMs) for the removal of Hg(II) ions from aqueous solution, using as the ligand di-(2-ethylhexyl)dithiophosphoric acid (DTPA). The effects on the removal percentage of Hg(ll) of variables such as pH, the nature of the acid, the concentration of mercury (in the feed solutions), and the ligand content (in the membrane) as well as the total surface membrane area exposed to the Hg(II) aqueous solution were studied. During the course of the removal experiments, the membrane was immersed in the Hg(II) aqueous solution in acid media and samples of the solution were taken at different times to enable monitoring of the mercury concentration changes. Itwasfound that when the ACM was prepared with a 1.0 M DTPA casting solution and the feed solution contained 2.49 x 10(-4) M Hg(II) in HCI 0.1 M the amount of mercury extracted was higher than 93%. Straight forwardly, additional experiments were carried out with the free-DTPA composite membranes to make up a set of control reference points to verify that removal of the investigated heavy metal was a consequence of the presence of the organic ligand; otherwise there was no Hg(II) concentration variation at all.
