ABSTRACT
OBJECTIVES: To detect and enumerate circulating prostatic tumor cells (CTC) in the peripheral blood of patients with prostate cancer (PC) and study the relationship between CTCs and clinical-pathological parameters. METHODS: Prospective three-arm study: 26 patients (p) with localised PC (LPC); 24 P with metastatic PC (MPC) and 30 healthy volunteer controls. A single 7.5 ml sample of peripheral blood was retrieved; CTCs were isolated using an immunomagnetic method based on the CellSearch system (Veridex). CTCs were identified as nucleated cells negative for CD45 (leukocytes) and positive for cytokeratins. (8, 18 y 19) The relationship between CTC numbers and PSA levels, Gleason score and TNM classification was studied. RESULTS: Only 10% of the healthy controls had 1 CTC/7.5 mL, none of the patients with localised PC had more than 3 CTCs (88% < or = 2 CTCs), and patients with MPC had significantly higher CTC levels [m: 29 (1-178)] compared with the other two groups (P: 0.000). A positive correlation was demonstrated between the CTC count and PSA levels, tumor size, and presence or absence of enlarged lymph nodes. Gleason score was the only parameter that did not show any correlation with CTC levels, and although the number of CTCs was higher in patients with visceral metastases [m: 297 (0-416)] compared with bone metastases patients [m: 68 (9.5-168)] , these differences were not significant. CONCLUSIONS: Immunomagnetic analysis permits CTCs to be enumerated in peripheral blood and could be a possible way to correctly stage and make a reasonable prognosis of metastatic disease.
Subject(s)
Immunomagnetic Separation , Neoplastic Cells, Circulating/pathology , Prostatic Neoplasms/pathology , Adult , Aged , Aged, 80 and over , Cell Count/methods , Humans , Male , Middle Aged , Prospective StudiesABSTRACT
OBJETIVOS: Detección y cuantificación de células tumorales prostáticas circulantes (CTC) en sangre periférica de pacientes con cáncer de próstata (CP) y estudiar la relación de las CTCs con los parámetrosclínico-patológicos.MÉTODOS: Estudio prospectivo con tres brazos: 26 pacientes (p) con CP localizado (CPL); 24p con CP metastático(CPM) y 30 controles voluntarios sanos. Se extrajouna única muestra de 7,5 mL de sangre periférica y se aislaron las CTC según un método inmunomagnéticobasado en el sistema CellSearch (Veridex). Las CTCs fueron identificadas como células nucleadas negativas para el CD45 (leucocitos) y positivas para las citoqueratinas(8, 18 y 19). Se estudiaron las relaciones del número de CTCs con los niveles de PSA, Gleason y clasificación TNM.RESULTADOS: Sólo el 10% de controles sanos tenían 1 CTC/7,5 mL, ninguno de los pacientes con CP localizadotuvo más de 3 CTC (88% ≤ 2 CTC) y aquellos con CPM presentaban niveles de CTCs significativamente más altos [m: 29 (1-178)] comparados con los otros dos grupos (P: 0.000). Se demostró una correlación positiva entre el número de CTC y cifras de PSA, con el tamaño del tumor y con la presencia o no de adenopatías.El grado Gleason fue el único parámetro que no mostró correlación con los niveles de CTC y aunque el número de CTC fue mayor en aquellos con metástasis viscerales [m: 297 (0-416)] comparado con los que tenían metástasis óseas [m: 68 (9,5-168)] estas diferenciasno fueron significativas.CONCLUSIONES: El análisis inmunomagnético nos permite cuantificar las CTC en sangre periférica y podríapresentar una posibilidad para lograr una estadificacióncorrecta y estimar un pronóstico adecuado de la enfermedad metastática(AU)
OBJECTIVES: To detect and enumerate circulating prostatic tumor cells (CTC) in the peripheral blood of patients with prostate cancer (PC) and study the relationship between CTCs and clinical-pathological parameters. METHODS: Prospective three-arm study: 26 patients (p) with localised PC (LPC); 24 P with metastatic PC (MPC) and 30 healthy volunteer controls. A single 7.5 ml sample of peripheral blood was retrieved; CTCs were isolated using an immunomagnetic method based on the CellSearch system (Veridex). CTCs were identified as nucleated cells negative for CD45 (leukocytes) and positive for cytokeratins. (8, 18 y 19) The relationship between CTC numbers and PSA levels, Gleason score and TNM classification was studied.RESULTS: Only 10% of the healthy controls had 1 CTC/7.5 mL, none of the patients with localised PC had more than 3 CTCs (88% ≤ 2 CTCs), and patients with MPC had significantly higher CTC levels [m: 29 (1-178)] compared with the other two groups (P: 0.000). A positive correlation was demonstrated between the CTC count and PSA levels, tumor size, and presen-ce or absence of enlarged lymph nodes. Gleason score was the only parameter that did not show any correlation with CTC levels, and although the number of CTCs was higher in patients with visceral metasta-ses [m: 297 (0-416)] compared with bone metastases patients [m: 68 (9.5-168)], these differences were not significant(AU)
Subject(s)
Humans , Male , Middle Aged , Immunomagnetic Separation/methods , Neoplastic Cells, Circulating/pathology , Prostatic Neoplasms/diagnosis , Prostatic Neoplasms/immunology , Adenocarcinoma/complications , Adenocarcinoma/diagnosis , Prostatectomy/methods , Neoplasm Staging/methods , Prospective Studies , FluoresceinABSTRACT
This study was designed to establish the role of microsatellite instability (MSI) in the development of sporadic tumors of the ovary. The instability of 6 microsatellites (BAT25, BAT26, NME1, D17S250, D5S346 and D2S123) was determined by comparing MSI in healthy and tumoral tissue in each of 40 patients undergoing surgery for a sporadic ovarian tumor. BAT26 and D2S123 instability was detected in borderline tumors, and ovarian carcinomas were found to present instability in the microsatellites BAT25, NME1 and D17S250. Our findings indicate that microsatellite instability lacks a significant role in the appearance or progression of sporadic ovarian tumors.
