[Characteristics of infection in children during prolonged oxidative stress after radiation exposure]. / Osobennosti infektsionnogo protsessa u detei v usloviiakh dlitel'nogo okislitel'nogo stressa vsledstvie radiatsionnogo vozdeistviia.

In the article on discusses the problems of origin and duration of chlamydia and herpes-virus infection in children with chronic somatic pathology and different degrees of radiation stress in contaminated territories of Brianskaya region (the level of contamination according to 137Cs = 9.37-19.73 Ci/km2).

[The diagnosis of herpesvirus infection in patients with organ allografts]. / Diagnostika gerpesvirusnoi infektsii u bol'nykh s allotransplantirovannymi organami.

The diagnostic value of three methods for detection of herpes virus infection caused by herpes simplex virus and cytomegaloviruses was investigated in patients with allotransplanted organs. The method of molecular hybridization was demonstrated to be more sensitive in diagnosing herpes virus infection than virological and cytological methods. Herpes virus infection can be effectively diagnosed by a complex of methods including the detection of cytomegalic cells in the urine and saliva.

[The incidence of detecting HB viral infection in the families of patients with kidney diseases]. / Chastota vyiavleniia HB-virusnoi infektsii v sem'iakh bol'nykh s zabolevaniiami pochek.

A total of 33 families were surveyed with a view to determine the presence of viral hepatitis B markers in persons with diagnosed renal diseases. In all patients markers of hepatitis B virus infection (HBsAg, anti-HBs antibodies, anti-HBc total and IgM antibodies) were determined in the enzyme immunoassay. Those families in which examinees with renal pathology were found to have markers of hepatitis B virus infection exhibited a high level of contamination with hepatitis B virus. A high proportion of examinees with viral hepatitis B was established in the families where mothers were found to have markers of hepatitis B virus infection. These facts indicate that everyday contacts play an important role in the transmission of the virus. The study points out that in the family foci of hepatitis B virus infection non-manifest or slightly manifest forms of this infection are mainly registered.

[A comparison of the results of analyzing the clinical data from gynecological, ophthalmological and dental patients using a set of rapid methods for the diagnosis of herpesvirus infection]. / Sopostavlenie rezul'tatov analiza klinicheskikh materialov ginekologicheskogo, oftal'mologicheskogo i stomatologicheskogo profilei naborom ékspress-metodov diagnostiki gerpesvirusnoi infektsii.

Analysis of biological specimens from gynecological, ophthalmological, and stomatological patients was carried out using current express methods for diagnosis of herpes virus infection developed by the authors: enzyme immunoassay (EIA), time-resolved fluoroimmunoassay (tr-FIA), molecular hybridization and conventional immunofluorescence technique. Monoclonal antibodies (MCA) generated by us with epitope specificity for gpD HSV-2 were used in EIA and tr-FIA. F(ab')2-fragments of MCA used as sorbents and for preparation of conjugates were found to be by 250% more sensitive than intact IgG used for similar purposes. The employment of EIA based on MCA F(ab')2-fragments contributed to detection of 0.2-0.6 ng of virus-specific protein in a specimen. Comparison of the results of analysis of clinical specimens of broad spectrum (mucous secreta from the cervical canal of the uterus and posterior vault of the vagina, saliva, lacrimal fluid) by the above express methods for diagnosis of herpes virus infection revealed a high percentage of positive findings, none of the methods providing 100% diagnosis. The use of a set of methods makes the resultant information more objective. Therefore, in future the data obtained by a number of current express methods for herpes diagnosis should be compared.

[Intrauterine and postnatal pneumonia in acquired immunodeficiency of infants]. / Vnutriutrobnye i postnatal'nye pnevmonii v usloviiakh priobretennogo immunodefitsita u detei rannego vozrasta.

The autopsy material of 15 children aged from 2 months to 3 years from the zonal group of increased risk of the ecologic pathology, acquired immunodeficiency and viral infections was assessed morphologically and clinically. Decreased number of T-cells (T4, T8), an increase of the level of serum IgA, IgE and immune complexes, HIV-antibodies (4 cases) were found in the patients. The method of the molecular hybridization by means of virus-specific 32P-DNA probes was used. Bronchopneumonia was the cause of death. Severe deficiency of the organs and cells of the immune system, alternative-proliferative lung inflammation, mainly in the form of pneumonitis and alveolitis, were found. The latter differed either individually or as a result of the predominant infectious agent (RNA- or DNA-viruses, pneumocysts, bacterial flora, fungi). Considerable immunity dysfunctions enhanced the intensity of the specific features in pneumonia morphology.

[The role of Chlamydia infection in the development of infectious inflammatory diseases in premature newborn infants]. / Znachenie infitsirovaniia khlamidiami v formirovanii infektsionno-vospalitel'nykh zabolevanii u nedonoshennykh novorozhdennykh detei.

It is highly probable that neonates may be infected with chlamydiae provided their mothers are chlamydia carriers, especially in disease exacerbation before delivery. Parallelism was noted between the intensity of antigen demonstration in the mother and the child. Infection with chlamydiae potentiates secondary infectious inflammatory diseases in premature neonates. However, diseases of chlamydia etiology developed only in 20.5% of children of all the infected and manifested themselves by pneumonias or total toxicosis without any localized focus of infection.

[Regularities of the content and organization of pyrimidine oligonucleotide sequences in insect DNA]. / Zakonomernosti v soderzhanii i organizatsii pirimidinovykh oligonukleotidnykh posledovatel'nostei v DNK nasekomykh.

Certain regularities in content and organization of pyrimidine oligonucleotide sequences of DNA from 15 insect species belonging to 4 orders were studied. The degree of nucleotide clusterization in insect DNA was found to be species-specific, being the highest in Hymenoptera and lowest in Lepidoptera; the Blattodea and Coleoptera occupy an intermediate position by this index between them. The changes in the DNA cluster structure during the evolution of insect species are not of vector type; the degree of clusterization of DNA nucleotide is either increased (Hymenoptera) or decreased (Lepidoptera as compared with Blattodea). In the DNA oligonucleotide fractions containing both pyrimidine nucleotides the percentage content of thymidyl nucleotides is much higher than that of cytidyl nucleotides, the thymine content being increased with the lengthening of oligopyrimidine clusters. The insect species with a higher degree of clusterization of DNA pyrimidine nucleotides contain more thymidyl nucleotide residues. These results agree well with the hypothesis suggesting that during the evolution of large taxons the accumulation of long pyrimidine sequences in animal DNA is accompanied by an increase of thymidyl nucleotide content in them. This can largely be due to the increase of matrix resistance during the evolution and is biologically significant for animals of any taxons, including insects.