Unlocking the potential of biosurfactants: Production, applications, market challenges, and opportunities for agro-industrial waste valorization.

Biosurfactants are eco-friendly compounds with unique properties and promising potential as sustainable alternatives to chemical surfactants. The current review explores the multifaceted nature of biosurfactant production and applications, highlighting key fermentative parameters and microorganisms able to convert carbon-containing sources into biosurfactants. A spotlight is given on biosurfactants' obstacles in the global market, focusing on production costs and the challenges of large-scale synthesis. Innovative approaches to valorizing agro-industrial waste were discussed, documenting the utilization of lignocellulosic waste, food waste, oily waste, and agro-industrial wastewater in the segment. This strategy strongly contributes to large-scale, cost-effective, and environmentally friendly biosurfactant production, while the recent advances in waste valorization pave the way for a sustainable society.

Biosurfactant Production from Pineapple Waste and Application of Experimental Design and Statistical Analysis.

The use of non-conventional carbon sources for biosurfactant-producing microorganisms is a promising alternative in fermentation to substitute costly substrates. So, the current research used pineapple peel as a cost-effective and renewable substrate because of its rich composition in minerals and sugars and high availability. Following a 22 full factorial design, a bacterial strain of Bacillus subtilis produced biosurfactants in fermentative media containing different concentrations of glucose and concentrated pineapple peel juice (CPPJ). The influence of these two independent variables was evaluated according to three different responses: surface tension reduction rate (STRR), emulsification index (EI24), and concentration of semi-purified biosurfactant (SPB). The maximum value for STRR (57.63%) was obtained in media containing 0.58% glucose (w/v) and 5.82% CPPJ (v/v), while the highest EI24 response (58.60%) was observed at 2% glucose (w/v) and 20% CPPJ (v/v) and maximum SPB (1.28 g/L) at 3.42% glucose (w/v) and 34.18% CPPJ (v/v). Statistical analysis indicated that the CPPJ variable mostly influenced the STRR and SPB responses, whereas the EI24 was significantly influenced by pineapple peel juice and glucose contents.

Handheld Echocardiography in a Clinical Practice Scenario: Concordances Compared to Standard Echocardiographic Reports.

BACKGROUND: The purpose of this study was to assess the utility of a handheld device (HH) used during common daily practice and its agreement with the results of a standard echocardiography study (STD) performed by experienced sonographers and echocardiographer. METHODS: A prospective follow-up was conducted in an adult outpatient echocardiography clinic. Experienced sonographers performed the STD and an experienced echocardiographer performed the HH. STD included 2-dimensional images, Doppler and hemodynamics analysis. Hemodynamic assessment was not performed with the HH device because the HH does not include such technology. The images were interpreted by blinded echocardiographers, and the agreement between the reports was analyzed. RESULTS: A total of 108 patients were included; and the concordance for left ventricle (LV) ejection fraction (EF), wall motion score index, LV and right ventricle (RV) function, RV size, and mitral and aortic stenosis was excellent with κ values greater than 0.80. Wall motion abnormalities had good concordance (κ value 0.78). The agreement for LV hypertrophy, mitral and aortic regurgitation was moderate, and tricuspid and pulmonary regurgitation agreements were low (κ values of 0.26 and 0.25, respectively). CONCLUSIONS: In a daily practice scenario with experienced hands, HH demonstrated good correlation for most echocardiography indications, such as ventricular size and function assessment and stenosis valve lesion analyses.

Effects of SNVs in ABCA1, ABCG1, ABCG5, ABCG8, and SCARB1 Genes on Plasma Lipids, Lipoproteins, and Adiposity Markers in a Brazilian Population.

Several proteins are involved in cholesterol homeostasis, as scavenger receptor class B type I and ATP-binding cassette (ABC) transporters including ABCA1, ABCG1, ABCG5, and ABCG8. This study aimed to determine the effects of single nucleotide variants (SNVs) rs2275543 (ABCA1), rs1893590 (ABCG1), rs6720173 (ABCG5), rs6544718 (ABCG8), and rs5888 (SCARB1) on plasma lipids, lipoproteins, and adiposity markers in an asymptomatic population and its sex-specific effects. Volunteers (n = 590) were selected and plasma lipids, lipoproteins, and adiposity markers (waist-to-hip and waist-to-height ratios, lipid accumulation product and body adiposity index) were measured. Genomic DNA was isolated from peripheral blood cells according to the method adapted from Gross-Bellard. SNVs were detected in the TaqMan® OpenArray® Real-Time polymerase chain reaction platform and data analyses were performed using the TaqMan® Genotyper Software. The rs2275543*C point to an increase of high-density lipoprotein size in females while in males very-low-density lipoprotein, cholesterol, and triglycerides were statistically lower (P value < 0.05). The rs1893590*C was statistically associated with lower apolipoprotein A-I levels and higher activities of paraoxonase-1 and cholesteryl ester transfer protein (P value < 0.05). The rs6720173 was statistically associated with an increase in cholesterol and low-density lipoprotein cholesterol in males; moreover, rs6544718*T reduced adiposity markers in females (P value < 0.05). Regarding the rs5888, a decreased adiposity marker in the total population and in females occurred (P value < 0.05). Multivariate analysis of variance showed that SNVs could influence components of high-density lipoprotein metabolism, mainly through ABCG1 (P value < 0.05). The ABCA1 and ABCG5 variants showed sex-specific effects on lipids and lipoproteins, while SCARB1 and ABCG8 variants might influence adiposity markers in females. Our data indicate a possible role of ABCG1 on HDL metabolism.

Biosurfactants produced from corncob: a bibliometric perspective of a renewable and promising substrate.

The reuse of agro-industrial waste has been a recurring issue since the 20th century. With a composition rich in carbohydrates and because of the massive amount of residue produced daily all over the world, corncob became a low-cost and suitable substrate to produce high added-value compounds. Biosurfactants are bioproducts of versatile applications due to their chemical structure with hydrophilic and hydrophobic regions. The current work performed a bibliometric analysis to identify research related to the synthesis of biosurfactants using corncob as substrate. Despite the high availability of corncobs, only nine articles were found in Scopus and Web of Science using different pretreatment processes and microorganisms. After an initial screening, data regarding research organizations, scientific journals, citations, countries, institutions, and keywords were analyzed. Results indicated that corncobs were also used to produce enzymes, adsorbents, activated carbon, and furfural. The presented evaluation updated the status of art, identifying a serious need for more research, especially because of corncob's high potential to provide fermentable sugars and the wide range of variables influencing fermentation processes that still need to be studied. A future association of this low-cost substrate with other methods can result in a promising scenario for technology transference.

Potential of pineapple peel in the alternative composition of culture media for biosurfactant production.

The large pineapple's consumption and processing have generated a massive amount of waste yearly, which requires adequate treatment measures to avoid damages to the environment. Pineapple peel is one of the main residues obtained from this fruit and a promising strategy to take advantage of its potential is using it for biosurfactant production due to the peel's rich composition in fermentable sugars and nutrients, such as potassium and magnesium that favor the Bacillus subtilis growth and biosurfactant excretion as well. The current research performed a central composite design (CCD) with four independent variables (glucose, pineapple peel, potassium, and magnesium), evaluating substrates' influence on the surface tension reduction rate (STRR) and the emulsification index (EI24). The results indicated that pineapple peel has the necessary potential to act as a partial substitute for glucose and salt nutrients, minimizing the costs of supplementing with exogenous minerals. The highest surface tension reduction rate (57.744%) was obtained at 2.18% glucose (w/v); 14.67% pineapple peel (v/v); 2.38 g/L KH2PO4; and 0.15 g/L MgSO4.7H2O; whereas to obtain the maximum predicted value for EI24 (61.92%) the medium was composed by 2.24% glucose (w/v); 12.63% pineapple peel (v/v); 2.53 g/L KH2PO4; and 0.29 g/L MgSO4.7H2O.

Evaluation of a new strategy in the elaboration of culture media to produce surfactin from hemicellulosic corncob liquor.

The biosurfactant production is characterized by high costs with substrates, which does not make them sufficiently competitive against synthetic surfactants. The insertion of alternative sources of low cost, especially agro-industrial residue, is an excellent alternative to make this competitiveness viable. An alkaline pretreatment was used to extract the hemicellulose from corncob in order to enhance its C5 fraction, common to vegetable biomasses. The hemicellulosic corncob liquor was used with glucose and mineral salt solution as carbon and nutrients sources in a fermentation process for the growth of Bacillus subtilis. It was performed a 23 full factorial design to determine the best conditions for the surfactin production in relation to the following response variables: surface tension reduction rate (STRR) and emulsification index (EI24), from which were obtained two optimized bioproducts under specific conditions. The optimized biosurfactants found to be effected presenting a critical micelle concentration of 100 mg.L-1 and a maximum bioremediation potential of 85.18%, as well as maximum values of 57.38% and 65.30% for STRR and EI24 variables, respectively. Overall results pointed for a successful commercial application for the surfactin produced.

Burnout e estresse: entre medicalização e psicologização / Burnout and stress: between medicalization and psychologization

Resumo Em 1974, o conceito de burnout surgiu para nomear o esgotamento típico das profissões de cuidado. É geralmente definido como síndrome psicológica decorrente do estresse crônico laboral, composta por três dimensões: exaustão emocional, despersonalização/cinismo e baixa realização pessoal. Este artigo analisa o burnout em uma perspectiva sociocultural, a partir dos referenciais teóricos de Loriol/Elias e Duarte/Dumont, investigando sua difusão através da associação conceitual com a noção de estresse laboral, ligada, por sua vez, à individualização e aos processos de medicalização/psicologização. Apesar da origem nas ciências físicas, a força da categoria estresse se deve muito menos a seu caráter técnico (teórico) que a seu aspecto simbólico. Ao promover uma linguagem comum entre biologização e psicologização, o estresse é capaz de circular nos mais diversos meios, do acadêmico ao senso comum, integrando em um código próprio ora representações psicologizadas, ora representações não psicologizadas de pessoa e perturbação. Pode assim funcionar como uma categoria "biopsicossocial". São essas características que "abrem caminho" para a difusão social do burnout, na medida em que ele é concebido como um tipo de estresse laboral - uma experiência que se encontra, hoje em dia, extremamente difundida, sendo vivenciada como parte da vida.

Abstract In 1974, the concept of burnout was created to express the exhaustion typical of helping professions. Generally defined as a psychological syndrome resulting from chronic occupational stress, it is composed by three dimensions: emotional exhaustion, depersonalization/cynicism, and low personal accomplishment. This article analyzes burnout from a sociocultural perspective, based on the theoretical frameworks of Loriol/Elias and Duarte/Dumont, investigating its diffusion by means of a conceptual association with the idea of work stress, which may also be associated to the processes of individualization and medicalization/psychologization. Despite its origins in the physical sciences, the category "stress" owes its strength less to its technical (theoretic) character than to its symbolic aspect. By promoting a common language between biologization and psychologization, the category stress is present in many different environments, from academic discussions to common sense conversation, integrating in a particular code psychologized and non-psychologized representations of the person and of physical and moral distress. It can therefore serve as a "biopsychosocial" category. These characteristics pave the way to burnout's social diffusion, as far as it is conceived as a type of work stress - an experience that, nowadays, is regarded as part of normal life.

Effects of scale and Froude number on the hydraulics of waste stabilization ponds.

This paper presents the findings from a series of computational fluid dynamics simulations to estimate the effect of scale and Froude number on hydraulic performance and effluent pollutant fraction of scaled waste stabilization ponds designed using Froude similarity. Prior to its application, the model was verified by comparing the computational and experimental results of a model scaled pond, showing good agreement and confirming that the model accurately reproduces the hydrodynamics and tracer transport processes. Our results showed that the scale and the interaction between scale and Froude number has an effect on the hydraulics of ponds. At 1:5 scale, the increase of scale increased short-circuiting and decreased mixing. Furthermore, at 1:10 scale, the increase of scale decreased the effluent pollutant fraction. Since the Reynolds effect cannot be ignored, a ratio of Reynolds and Froude numbers was suggested to predict the effluent pollutant fraction for flows with different Reynolds numbers.

O papel do dolutegravir na terapia antiretroviral / The role of dolutegravir in antiretroviral therapy

os inibidores da integrase são a mais nova classe de antirretroviral aprovada, que agem impedindo a incorporação do DNA do HIV no genoma do linfócito T CD4+ (LTCD4+) do hospedeiro, limitando a propagação do vírus. o Dolutegravir e o inibidor da integrase mais moderno e como os demais inibidores apresenta de alta performance, boa tolerância; alta barreira genética para mutações de resistência, além de apresentar eficácia em pacientes já submetidos a tratamento antirretroviral anterior. Neste contexto o presente estudo trata-se de um estudo de revisão bibliográfica realizada de janeiro a junho de 2018, de artigos científicos de artigos científicos que abordam aspectos exclusivos do dolutegravir na terapia antirretroviral em comparação com outros esquemas terapêuticos. Concluindo que o tratamento com dolutegravir apresenta como principais vantagens à rápida supressão virológica; boa tolerância e alta barreira genética para mutações de resistência.

Integrase inhibitors are the newest class of approved antiretroviral drugs that act by preventing the incorporation of HIV DNA into the CD4 + T lymphocyte (LTCD4 +) genome of the host, limiting the spread of the virus. Dolutegravir and the most modern integrase inhibitor and like the other inhibitors presents high performance, good tolerance; high genetic barrier for resistance mutations, in addition to being effective in patients already submitted to previous antiretroviral treatment. In this context, the present study is a bibliographical review study conducted from January to June, 2018, of scientific papers on scientific articles dealing with exclusive aspects of dolutegravir in antiretroviral therapy compared to other therapeutic regimens. Concluding that dolutegravir treatment has the main advantages of rapid virological suppression; good tolerance and high genetic barrier for resistance mutations

Diversos nomes para o cansaço: categorias emergentes e sua relação com o mundo do trabalho / Several names for tiredness: emergent categories and their relationship with the world of work / Diversos nombres para el cansancio: categorías emergentes y su relación con el mundo del trabajo

Este artigo tem como objetivo explorar, do ponto de vista sociocultural, a emergência de duas entidades clínicas entre 1970 e 1980 que, no campo da clínica geral e dos saberes psi, nomeiam quadros centrados no sintoma da fadiga: a síndrome da fadiga crônica (CID-10 G93.3) e o burnout (CID-10 Z73.0). Pretendemos, após uma breve apresentação de ambas, analisar o surgimento dessas categorias, para indicar o cansaço e a exaustão como sintomas a serem tratados medicamente e explorar os pontos de aproximação e afastamento entre elas...

The objective of this article was, from a sociocultural perspective, to explore the emergence of two clinical entities between 1970 and 1980, which, in the fields of general clinical medicine and psycho-knowledge, give names to conditions centered on the symptom of fatigue: chronic fatigue syndrome (ICD-10 G93.3) and burnout (ICD-10 Z73.0). We intend, after a brief presentation of both of these, to analyze the emergence of these two categories, to acknowledge fatigue and exhaustion as symptoms to be medically treated, and to explore similarities and differences between them...

El objetivo de este artículo es explorar, desde el punto de vista sociocultural, la emergencia de dos entidades clínicas entre 1970 y 1980 que, en el campo de la clínica general y de los saberes psi, dan nombre a cuadros centrados en el síntoma de la fatiga: el síndrome de la fatiga crónica (CID-10 G93.3) y el burnout (CID-10 Z73.0). Pretendemos, después de una breve presentación de ambas, analizar el surgimiento de esas categorías, para indicar el cansancio y el agotamiento como síntomas que hay que tratar médicamente y explorar los puntos de aproximación y de separación entre ellas...

p.Q192R SNP of PON1 seems not to be Associated with Carotid Atherosclerosis Risk Factors in an Asymptomatic and Normolipidemic Brazilian Population Sample / O SNP p.Q192R da PON1 não Parece Associar-se a Fatores de Risco para a Aterosclerose Carotídea em Amostra de População Brasileira Normolipidêmica e Assintomática

Evidences suggest that paraoxonase 1 (PON1) confers important antioxidant and anti-inflammatory properties when associated with high-density lipoprotein (HDL).

p.Q192R SNP of PON1 seems not to be Associated with Carotid Atherosclerosis Risk Factors in an Asymptomatic and Normolipidemic Brazilian Population Sample.

BACKGROUND: Evidences suggest that paraoxonase 1 (PON1) confers important antioxidant and anti-inflammatory properties when associated with high-density lipoprotein (HDL). OBJECTIVE: To investigate the relationships between p.Q192R SNP of PON1, biochemical parameters and carotid atherosclerosis in an asymptomatic, normolipidemic Brazilian population sample. METHODS: We studied 584 volunteers (females n = 326, males n = 258; 19-75 years of age). Total genomic DNA was extracted and SNP was detected in the TaqMan® SNP OpenArray® genotyping platform (Applied Biosystems, Foster City, CA). Plasma lipoproteins and apolipoproteins were determined and PON1 activity was measured using paraoxon as a substrate. High-resolution ß-mode ultrasonography was used to measure cIMT and the presence of carotid atherosclerotic plaques in a subgroup of individuals (n = 317). RESULTS: The presence of p.192Q was associated with a significant increase in PON1 activity (RR = 12.30 (11.38); RQ = 46.96 (22.35); QQ = 85.35 (24.83) µmol/min; p < 0.0001), HDL-C (RR= 45 (37); RQ = 62 (39); QQ = 69 (29) mg/dL; p < 0.001) and apo A-I (RR = 140.76 ± 36.39; RQ = 147.62 ± 36.92; QQ = 147.49 ± 36.65 mg/dL; p = 0.019). Stepwise regression analysis revealed that heterozygous and p.192Q carriers influenced by 58% PON1 activity towards paraoxon. The univariate linear regression analysis demonstrated that p.Q192R SNP was not associated with mean cIMT; as a result, in the multiple regression analysis, no variables were selected with 5% significance. In logistic regression analysis, the studied parameters were not associated with the presence of carotid plaques. CONCLUSION: In low-risk individuals, the presence of the p.192Q variant of PON1 is associated with a beneficial plasma lipid profile but not with carotid atherosclerosis.

Chemical modification of high density lipoprotein subfractions - HDL2 and HDL3 - after use of atorvastatin.

Regardless of its effect on the concentrations of serum cholesterol, statins exert pleiotropic effects, including the regulation of endothelial function, reduced oxidative stress and inflammation, as well as a slight improvement in the concentrations of high density lipoprotein (HDL). However, its role on the composition of HDL is not yet established. The aim of this study was to evaluate the composition of HDL subfractions, HDLsub>2 and HDL3, after 14 days of placebo and atorvastatin (10 mg/day) use in 30 asymptomatic volunteers. The serum parameters and the HDL subfractions compositions were determined using radiometric, nephelometric and biochemical enzymatic methods. We observed significant reductions of total cholesterol, low density lipoprotein (LDL) and apolipoprotein B-100 by 28%, 40% and 38%, respectively. The analyses of chemical composition of the subfractions revealed a lower lipid protein ratio in HDL2, suggesting enrichment in proteins, and also lower in HDL3, probably by an increase in the number of particles. Several mechanisms can be suggested for the effects observed after the use of atorvastatin, such as a possible action on the reverse cholesterol transport (decreased activity of hepatic lipase and increased phospholipid transfer protein, PLTP), which would explain the enrichment of HDL. The results suggest that statin use may be relevant in the primary prevention of atherosclerosis not only by its lowering effect on LDLcholesterol and its anti-inflammatory effect but also by beneficial changes in HDL subfractions.

Impact of seasonality on the prevalence of dyslipidemia: a large population study.

Assessment of lipid profile parameters has been considered a cornerstone in classifying individuals and populations at risk for cardiovascular disease. Recently, however, preliminary data have raised the possibility of seasonal variations in these parameters, which may cause under- or overestimation. Biological rhythms and seasonal variation of lipid profile was investigated in 227 359 consecutive individuals who underwent health checkups in primary care centers between 2008 and 2010. Plasma low-density lipoprotein cholesterol (LDL-C) >130 mg/dL was 8% more prevalent during winter than summer, with a larger difference among women and middle-aged adults (p < 0.001). High-density lipoprotein cholesterol (HDL-C) <40 mg/dL and triglycerides (TG) >150 mg/dL were respectively 9% and 5% more prevalent during the summer (p < 0.001). Variation amplitude was 3.4 ± 0.3 mg/dL for HDL-C (p = 0.005), 7 ± 2 mg/dL for LDL-C (p = 0.047), and 12 ± 9 mg/dL for TG (p = 0.058). Based on a large population sample, this study confirms the existence of biological rhythms and seasonal variation in lipid profile. This finding must be particularly accounted for in cross-sectional analyses of relative risk, prevalence, or the rate of goal achievement for lipid parameters.

Development of a clinical laboratory data base of hyper and hypo alpha lipoproteins in Campinas-SP and neighboring region / Construção de um banco de dados clínico laboratorial de hiper e hipoalfalipoproteinêmicos em Campinas-SP e região

INTRODUCTION: The development of research for diagnosis, prevention and treatment of atherosclerotic cardiovascular disease is of utmost importance due to the fact that it is the main cause of morbidity and mortality in Brazil. OBJECTIVE: To demonstrate the phases of the selection process for candidates with the aim to develop a clinical-laboratorial database of hyper alpha lipoproteinemic patients (hyper A) - high density lipoprotein cholesterol (HDL-C) ≥ 68 mg/dl) and hypo alpha lipoproteinemic patients (hypo A) - HDL-C < 39 mg/dl. MATERIAL AND METHODS: The volunteers were contacted after selection of lipid profiles from individuals treated at the Sistema Único de Saúde (SUS), Campinas-SP and neighboring area. Afterwards, the selected patients went through blood collection, clinical examinations and answered questionnaires on dietary frequency and physical activity. After this preliminary evaluation, some individuals were convened to another blood collection and, subsequently, were submitted to an ultrasonographic exam of the carotid arteries. RESULTS: Only 0.6% and 0.3% from 598,288 lipid profiles were selected for hyper A and hypo A groups, respectively, including gender disparity. Lack of effective questionnaires (75%), missing calls (60%) and non-inclusion were the major hindrances in the construction of this database. DISCUSSION: The difficulties to obtain eligible candidates were also due to the low prevalence of both groups hypo A and hyper A and the high prevalence of pathologies that contribute to non-genetic variations of HDL-C. CONCLUSION: In spite of the obstacles in the development of this database, this study brought about several scientific publications. Furthermore, the development of molecular analyzes and functionality will shortly generate other findings, contributing to the diagnosis and follow-up of HDL dyslipidemias.

INTRODUÇÃO: O desenvolvimento de pesquisa para diagnóstico e prevenção da doença aterosclerótica cardiovascular no Brasil é de grande importância por esta ser a principal causa de morbimortalidade no país. OBJETIVO: Demonstrar as etapas do processo de seleção de voluntários para a construção de um banco de dados clínico-laboratorial de indivíduos hiperalfalipoproteinêmicos (hiper A) - colesterol da lipoproteína de alta densidade (HDL-C) ≥ 68 mg/dl - e hipoalfalipoproteinêmicos (hipo A) - HDL-C < 39 mg/dl. MATERIAL E MÉTODOS: Os voluntários são contatados a partir de resultados de perfis lipídicos de indivíduos atendidos pelo Sistema Único de Saúde (SUS) de Campinas-SP e região e, se selecionados, são convidados para coleta de sangue, exames clínicos e responder a questionários de atividade física e de frequência alimentar. Após essa avaliação, os indivíduos podem ser convocados para nova coleta de sangue e, posteriormente, para a ultrassonografia de carótidas. RESULTADOS: Entre 598.288 perfis lipídicos recebidos das redes públicas, apenas 0,6% e 0,3% compuseram os nossos grupos hiper A e hipo A, com disparidade entre os gêneros. A falta de questionários efetivos (75%), das chamadas não atendidas (60%) e a não inclusão foram os pontos mais difíceis na construção do banco de dados. DISCUSSÃO: A dificuldade de obtenção de voluntários elegíveis também se deve à baixa prevalência de hipo A e hiper A e à alta prevalência de patologias que contribuem para variações não genéticas do HDL-C. CONCLUSÃO: Apesar das dificuldades na criação da base de dados, este estudo gerou várias publicações e, com o desenvolvimento das análises moleculares e da funcionalidade, muitas outras seguirão em curto período, fatos contribuintes para o diagnóstico e o acompanhamento das dislipidemias envolvendo a HDL.

Análise quantitativa de Cândida sp em portadores de prótese total no município de Cataguases/MG / Quantitative analysis of candida species in patients with complete dentures in the city of cataguases - MG (brazil)

Este estudo visou quantificar a presença de Cândida sp em indivíduos portadores de prótese total convencional de ambos os sexos, com idade entre 40 e 80 anos (média 60,32 ± 10,82 anos), usuários do Serviço de Prótese do Centro de Especialidades Odontológicas do município de Cataguases/MG. Especificamente, três espécies de Cândida sp foram quantificadas em indivíduos com presença e/ou ausência de lesões bucais. Participaram deste estudo, 22 pacientes que foram divididos em dois grupos: G1) usuários de prótese total sem lesões, onde foi coletado material apenas da prótese total e G2) usuários de prótese total com lesões, onde o material foi coletado da prótese total e da região que apresentou alguma lesão, com rigoroso padrão de biossegurança. As placas foram incubadas à temperatura de 35ºC e o crescimento acompanhado por até 48h. Houve prevalência de 77,30% no sexo feminino e de 81,81% nos leucodermas. A C. albicans obteve a maior média (2,13 ± 2,43) na prótese. A prova de Mann-Whitney apontou uma diferença estatisticamente significante (p = 0,039) para a C. albicans na prótese em relação às outras espécies e locais pesquisados. Concluiu-se que após a instalação das próteses totais, as lesões fúngicas podem ser prevenidas com orientações quanto aos métodos de higienização e manutenção da saúde bucal

This study aimed at quantifying the presence of Candida sp in individuals with conventional full denture from both gender, aged between 40 and 80 years (average 60.32 ± 10.82 years), users of the Dental Specialties Service Center in the city of Cataguases - MG. Three species of Candida were specifically quantified in individuals with and/or without absence of oral lesions. The study included 22 patients divided into two groups: G1) composed by denture users without lesions, with samples collected only from the denture and G2) composed by denture users with injuries, with samples collected from the denture and from the injured region according to rigorous standard of biosecurity. The plates were incubated at 35°C and growth monitored for up to 48 hours. There was a prevalence of 77.30% in females and 81.81% in Caucasian patients. C. albicans obtained the highest average (2.13 ± 2.43) in the prosthesis. The Mann-Whitney test showed a statistically significant difference (p = 0.039) for C. albicans in denture in comparison to other species and areas also tested. It was concluded that after the installation of dentures, fungal lesions can be prevented by guidance on methods of cleaning and maintenance of oral health

Diffusion MR imaging: an important tool in the assessment of brain tumors.

For over 20 years, conventional MR imaging has been used for assessing brain tumors. However, conventional MR imaging tends to underestimate the extent of the tumor, perhaps leading to suboptimal treatment. New MR imaging tools have been widely used to determine the grade, heterogeneity, and extent of brain tumors. Diffusion-weighted imaging has been studied extensively, helping in tumor grading, differential diagnosis, and postoperative evaluation. Diffusion tensor imaging can apparently delineate more accurately the tumor versus the infiltrating tumor between the peritumoral edema and the normal brain parenchyma. This article shows the main clinical applications of these sequences.

Implantodontia estética na região anterior da maxila - pilar metálico ou cerâmico? Uma revisão de literatura / Aesthetics implantology in maxillary anterior region - metallic or ceramic abutment? A review

Atualmente a exigência estética é predominante na odontologia inclusive na implantodontia. Este fato tem levado ao desenvolvimento de novas técnicas e materiais. Os pilares cerâmicos surgem como uma alternativa concreta nas reabilitações protéticas em regiões onde a estética é essencial. O objetivo desta revisão de literatura é avaliar e comparar as indicações, propriedades mecânicas, biocompatibilidade, adaptação e longevidade clínica dos pilares estéticos e metálicos. Os resultados deste estudo mostraram que os pilares metálicos ainda são os mais indicados na grande maioria dos casos protéticos, sem comprometimento da estética e da função, com exceção nos casos de linha de sorriso alto com gengiva delgada e/ou recessões

Currently the aesthetic is predominant in dentistry, even in implantology. This fact has led to development of new techniques and materials. Ceramics abutment appears to be a viable alternative in prosthetic rehabilitations in esthetic regions where the esthectic is important. The purpose of this review is to evaluate and compare the indications, mechanical properties, biocompatibility, adaptation and clinical performance of aesthetic and metal abutments. The results of this study showed that the metal abutments are the most suitable in most cases prosthetic, without compromising the aesthetics and function, except in cases of high smile line with thin gingival thin and / or recessions