Quality of broiler meat of the free-range type submitted to diets containing alternative feedstuffs / Qualidade da carne de frangos de corte do tipo caipira submetidos a dietas contendo alimentos alternativos

The work had the intention of investigating the effect of the use of alternative feeds as part replacers in diet-formulating, evaluating the characteristic physicochemical alterations and centesimal composition of the free-range chicken. In the experiment a total of 192 (one hundred and ninety-two) birds of the Pescoço Pelado (Label Rouge) strain arranged in a completely randomized design (CRD) formed by 4 treatments (Treatment 1 (Control), Treatment 2 (10% of the inclusion of rice bran), Treatment 3 (10% of the inclusion of ground cassava leaf) and Treatment 4 (10% of the inclusion of ground leucaena hay) with 8 replicates per treatment were used. The results revealed greater values of b* (yellow), Saturation (C*) and pH for broiler meat with inclusion of ground cassava leaf and leucaena, while for the other variables of physicochemical composition, no influences of the treatments were not found. For centesimal composition the treatments showed greater values of moisture in relation to the control treatment. For sex, only a difference for the content of b* and C* was found, with higher values for female. The use of the alternative feedstuffs has not revealed marked influences on the chemical composition and quality parameters of free-ranging chicken’s meat with the use of replacement up to 10% in the diets.

O trabalho teve como objetivo analisar o efeito do uso alimentos alternativos como substitutos parciais na formulação das rações, avaliando as alterações características físico-químicas e de composição centesimal de frango caipira. No experimento foi utilizado um total de 192 (cento e noventa e duas) aves da linhagem Pescoço Pelado (Label Rouge), sendo organizado através de um Delineamento Inteiramente ao acaso (DIC) formado por 4 tratamentos (Tratamento1 (Controle), Tratamento 2 (10% de inclusão de Farelo de Arroz), Tratamento 3 (10% de inclusão da Folha da Mandioca Triturada) e Tratamento 4 (10% da inclusão de Feno de Leucena Triturado) com 8 repetições por tratamento. Os resultados revelaram maiores valores de b* (amarelo), Índice de Saturação (C*) e pH para carne de frango com inclusão da Folha de Mandioca e Leucena triturada, enquanto as demais variáveis de composição físico-química não foram verificado influência dos tratamentos. Para composição centesimal os tratamentos apresentaram maiores valores de umidade em relação ao tratamento controle. Para sexo, somente foi verificada diferença para o teor de b* e C*, com maiores valores para fêmea. O uso dos ingredientes alternativos não revelaram influências marcantes sobre a composição química e parâmetros de qualidade das carnes de frango caipira com o uso de substituição até 10% nas rações.

Meat quality and lipid profiles in crossbred lambs finished on clover-rich pastures.

Meat quality traits were compared in Texel x Polwarth and Texel x Corriedale ram lambs, with live weight of 25kg, finished on pastures rich in white clover. The two crossbred groups showed similar results (P>0.05) for pH, moisture, protein, meat color, individual SFA and total SFA. Fat content was higher and cholesterol lower in Texel x Polwarth lambs (P<0.05), which also had higher amounts of MUFA (P<0.05) and lower levels of total PUFA and n-6 PUFA (P<0.01). Differences in meat quality among the two crossbred groups were minor, and possibly not perceptible from the consumer standpoint. However, both groups of lambs produced lean meat with high amounts of PUFA (>16%), possibly due to the ingestion of white clover in the finishing period. Overall, these results indicate that a differentiated product can be obtained in these conditions, with higher nutritional value than conventional lamb meat.

Study of p53 codon 72 polymorphism in patients with breast cancer.

Breast cancer is a common disease in Western societies, with an incidence of 46.31/100,000 women/year in Brazil. The tumor suppressor gene TP53 is one of the most studied genes regarding the presence of mutations. Indeed, 50% of all tumors are known to exhibit changes in the TP53 nucleotide sequence due to carcinogenic processes. As to the presence of polymorphism, the TP53 gene is polymorphic at the nucleotide residue 347 (codon 72). In the current study, we examine if this polymorphism is associated with the clinicopathological parameters of breast cancer patients in a Brazilian population. One hundred and thirteen patients with breast cancer were included. The polymorphic region of the TP53 gene was PCR-amplified from genomic DNA obtained from buccal cells. Specific primers for the Pro and Arg allele were used. Correlations of polymorphism with age, staging, nuclear grade, lymph node status, estrogen receptor status and lymphatic and/or blood vessel invasion were evaluated. Statistical analysis was performed using the Fisher's exact test. The frequency of p53 Arg/Arg was 57% and of the heterozygous allele Arg/Pro it was 39%. There was no correlation between polymorphism and clinicopathological parameters. According to our results, the TP53 polymorphism, at the 347 residue, is not associated with any clinicopathological findings of patients with breast cancer.

Results of surgery in patients with refractory extratemporal epilepsy with normal or nonlocalizing magnetic resonance findings investigated with subdural grids.

PURPOSE: To study the efficacy of extensive coverage of the brain surface with subdural grids in defining extratemporal cortical areas amenable for resection in patients with refractory extratemporal epilepy (R-ExTE) and normal or nonlocalizing magnetic resonance imaging (MRI) scans. METHODS: Sixteen patients with R-ExTE were studied. Eleven patients had simple partial, eight had complex partial, and three had supplementary motor area seizures. Seizure frequency ranged from three per month to daily episodes. Interictal EEG showed large focal spiking areas in 11 patients, secondary bilateral synchrony in four, and was normal in one patient. Surface ictal recordings were nonlocalizing in six patients, and in 10, they disclosed large ictal focal spiking areas. MRI was normal in 10 patients, and in six patients, focal nonlocalizing potentially epileptogenic lesions were found. All patients were given an extensive coverage of the cortical convexity with subdural electrodes through large unilateral (n = 13) or bilateral (n = 3) craniotomies. Bipolar cortical stimulation was carried out through the implanted electrodes. RESULTS: Interictal invasive recording findings showed widespread spiking areas in 13 patients and secondary bilateral synchrony in three. Ictal invasive recordings showed focal seizure onset in all patients. There were six frontal, two parietal, one temporooccipital, four rolandic, and three posterior quadrant resections. Thirteen patients had been rendered seizure free after surgery, and three had > or =90% of seizure-frequency reduction. Pathologic findings included gliosis (n = 10), cortical dysplasia (n = 5), or no abnormalities (n = 1). Six patients had transient postoperative neurologic morbidity. CONCLUSIONS: Extensive subdural electrodes coverage seems to be an effective way to investigate patients with R-ExTE and normal or nonlocalizing MRI findings.

Intravascular papillary endothelial hyperplasia causing cauda equina compression: case report.

OBJECTIVE AND IMPORTANCE: Intravascular papillary endothelial hyperplasia (Masson's vegetant hemangioendothelioma) is a rare condition affecting the neuraxis. In the literature, only one case of this lesion involving the vertebral canal with spinal cord compression has been reported. We present a case of cauda equina compression due to this abnormality. CLINICAL PRESENTATION: A 17-year-old boy was admitted at our hospital with pain, numbness, paresis of the left lower extremity, and bladder dysfunction of approximately 1 month's duration. Computed tomography and magnetic resonance imaging of the spine revealed a tumor within the spinal canal at the T12-L1 level. INTERVENTION: The patient underwent a T12-L1 laminectomy. An epidural red nodular tumor was visualized and totally resected. The findings of the pathological examination were compatible with intravascular papillary endothelial hyperplasia. At follow-up examination 1 month after the operation, the patient had complete resolution of the pain, and the motor deficit and bladder dysfunction had improved significantly. CONCLUSION: This rare benign vascular lesion may be clinically and histopathologically mistaken for an angiosarcoma. Because the intravascular papillary endothelial hyperplasia can be cured by complete surgical resection, it is important to distinguish between these two lesions to avoid inappropriate aggressive treatment.