Impact of chitosan-incorporated toothpaste on roughness, gloss, and antifungal potential of acrylic resin.

This study aimed to test the efficacy of different silica-based toothpastes with or without chitosan, as a method of cleaning the acrylic surfaces of denture prostheses. Acrylic resin specimens were prepared to evaluate surface roughness and gloss (n = 10), and Candida albicans adhesion/inhibition (n = 2). Two toothpastes with different degrees of abrasiveness were used: Colgate (CT) and Elmex (EX), with or without 0.5% chitosan (Ch) microparticles (CTCh or EXCh, respectively). The negative control was brushed with distilled water. Brushing was simulated with a machine. Surface roughness and gloss were analyzed before and after brushing. Candida albicans incidence/inhibition was tested qualitatively to determine the acrylic resin antifungal activity. The roughness and gloss data were analyzed with a generalized linear model, and the Kruskal Wallis and Dunn tests, respectively (α = 5%). Brushing with toothpastes increased roughness and reduced gloss, compared with the negative control (p < 0.05). CT showed a more significantly different change in roughness and gloss, in relation to the other groups (p < 0.05). Addition of chitosan to CT reduced its abrasive potential, and yielded results similar to those of EX and EXCh. Specimens brushed with CT showed a higher potential for Candida albicans adherence, despite its higher antifungal action. Addition of chitosan to the toothpaste made both toothpaste and brushing more effective in inhibiting Candida albicans. CT had the potential to increase roughness, reduce gloss, and increase Candida albicans adherence. In contrast, chitosan added to CT showed greater antifungal potential, and a higher synergistic effect than EX.

In-office tooth bleaching with chitosan-enriched hydrogen peroxide gels: in vitro results.

OBJECTIVE: This study aimed to evaluate the effects of adding chitosan to 35% hydrogen peroxide gels (for in-office bleaching), with or without calcium gluconate, on tooth properties and bleaching efficacy. METHODS: Bovine enamel/dentin specimens (4 × 4 × 2.5 mm) were randomly allocated into groups (n = 10): negative control (unbleached), bleaching with 35% hydrogen peroxide gel (35% HP, commercial gel); 35% HP with 2% chitosan (% wt) (35% HP + chitosan), 35% HP and calcium (35% HP + Ca, commercial gel), and 35% HP + Ca + 2% chitosan. Variation of surface profile (ΔRa) and color analyses (ΔL*, Δa*, Δb*, ΔE*ab, ΔE00, and ΔWID) were performed comparing specimens at baseline (initial) and 24 h after of storage in artificial saliva (final). Surface microhardness (KHN) values and scanning electron microscopy (SEM) images were obtained on conclusion. The data were analyzed by ANOVA and Tukey's tests (KHN), generalized linear models (ΔL*, ΔEab, ΔE00, ΔWID, ΔRa), and Kruskal-Wallis and Dunn tests (Δa*, Δb*) (α = 0.05). RESULTS: Considering ΔL*, Δa*, Δb*, ΔE*ab, ΔE00, and ΔWID values, the bleached groups differed from negative control. For ΔRa, chitosan-based groups showed lower variation in surface roughness compared to 35% HP, without significant difference from negative control. For KHN, chitosan groups did not differ from negative control (unbleached control = chitosan groups > 35% HP + Ca > 35% HP). For SEM, slight surface changes were observed in all bleached groups, but the intensity varied according to gel used (35% HP > gels with Ca > gels with chitosan). CONCLUSION: Chitosan-enriched hydrogen peroxide gels can reduce negative impacts on tooth properties without affecting bleaching efficacy. CLINICAL RELEVANCE: Although commercial gels containing remineralizing agents such as calcium reduce the negative effects on the properties of teeth, the addition of chitosan appears to be a promising approach to preservation of dental properties without interfering in bleaching efficacy.