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Does the DNA methylase Eco dam pair nucleotide sequences to form site-specific duplexes?
Zinoviev, V V; Vienozhinskis, M T; Malygin, E G; Nesterenko, V F; Popov, S G.
FEBS Lett ; 168(1): 166-8, 1984 Mar 12.
Article in English | MEDLINE | ID: mdl-6368265

ABSTRACT

The Eco dam methylase is active on denatured DNA and single-stranded synthetic oligonucleotides containing GATC sites. The results suggest that on interaction with single-stranded oligonucleotides the Eco dam methylase is able to form a duplex structure within the GATC site, and that this duplex site is a substrate for enzyme.


Subject(s)
Methyltransferases/metabolism , Animals , Base Sequence , Cattle , DNA , Escherichia coli/enzymology , Kinetics , Oligodeoxyribonucleotides , Site-Specific DNA-Methyltransferase (Adenine-Specific) , Substrate Specificity , Thymus Gland
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