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1.
Clin Genet ; 93(2): 206-215, 2018 02.
Article in English | MEDLINE | ID: mdl-28374897

ABSTRACT

Galactosemia type 1 is an autosomal recessive disorder of galactose metabolism, determined by a deficiency in the enzyme galactose-1-phosphate uridyltransferase (GALT). GALT deficiency is classified as severe or variant depending on biochemical phenotype, genotype and potential to develop acute and long-term complications. Neonatal symptoms usually resolve after galactose-restricted diet; however, some patients, despite the diet, can develop long-term complications, in particular when the GALT enzyme activity results absent or severely decreased. The mechanisms of acute and long-term complications are still discussed and several hypotheses are presented in the literature like enzymatic inhibition, osmotic stress, endoplasmic reticulum stress, oxidative stress, defects of glycosylation or epigenetic modification. This review summarizes the current knowledge of galactosemia, in particular the putative mechanisms of neonatal and long-term complications and the molecular genetics of GALT deficiency.


Subject(s)
Epigenesis, Genetic/genetics , Galactosemias/genetics , Oxidative Stress/genetics , UTP-Hexose-1-Phosphate Uridylyltransferase/genetics , Alleles , Galactosemias/pathology , Genotype , Glycosylation , Humans , Phenotype
2.
Gene ; 559(2): 112-8, 2015 Apr 01.
Article in English | MEDLINE | ID: mdl-25592817

ABSTRACT

Classical galactosemia is an autosomal recessive inborn error of metabolism due to mutations of the GALT gene leading to toxic accumulation of galactose and derived metabolites. With the benefit of early diagnosis by neonatal screening and early therapy, the acute presentation of classical galactosemia can be prevented. However, despite early diagnosis and treatment, the long term outcome for these patients is still unpredictable because they may go on to develop cognitive disability, speech problems, neurological and/or movement disorders and, in females, ovarian dysfunction. The objectives of the current study were to report our experience with a group of galactosemic patients identified through the neonatal screening programs in northeastern Italy during the last 30years. No neonatal deaths due to galactosemia complications occurred after the introduction of the neonatal screening program. However, despite the early diagnosis and dietary treatment, the patients with classical galactosemia showed one or more long-term complications. A total of 18 different variations in the GALT gene were found in the patient cohort: 12 missense, 2 frameshift, 1 nonsense, 1 deletion, 1 silent variation, and 1 intronic. Six (p.R33P, p.G83V, p.P244S, p.L267R, p.L267V, p.E271D) were new variations. The most common variation was p.Q188R (12 alleles, 31.5%), followed by p.K285N (6 alleles, 15.7%) and p.N314D (6 alleles, 15.7%). The other variations comprised 1 or 2 alleles. In the patients carrying a new mutation, the biochemical analysis of GALT activity in erythrocytes showed an activity of <1%. In silico analysis (SIFT, PolyPhen-2 and the computational analysis on the static protein structure) showed potentially damaging effects of the six new variations on the GALT protein, thus expanding the genetic spectrum of GALT variations in Italy. The study emphasizes the difficulty in establishing a genotype-phenotype correlation in classical galactosemia and underlines the importance of molecular diagnostic testing prior to making any treatment.


Subject(s)
Galactosemias/genetics , UDPglucose-Hexose-1-Phosphate Uridylyltransferase/genetics , Adolescent , Adult , Child , Child, Preschool , DNA Mutational Analysis , Female , Galactosemias/diagnosis , Genetic Association Studies , Humans , Infant , Infant, Newborn , Italy , Male , Mutation, Missense , Neonatal Screening , Young Adult
3.
Clin Genet ; 84(3): 265-70, 2013 Sep.
Article in English | MEDLINE | ID: mdl-23110537

ABSTRACT

Female carriers of Duchenne muscular dystrophy (DMD) are usually asymptomatic. However, 2.5-7.8% of them may present muscle symptoms and cardiomyopathy, attributed to a reduced production of dystrophin, probably because of skewed patterns of X-chromosome inactivation (XCI). To evaluate the role of XCI in symptomatic (at muscle or heart level) and asymptomatic DMD carriers, 44 subjects were selected from our database (12 manifesting, 21 non-manifesting, 11 healthy females), and XCI pattern determined in the lymphocytes by the androgen receptor methylation-based assay. The results showed that DMD-manifesting carriers had a preferential inactivation of the X-chromosome carrying the normal allele, while non-manifesting carriers and healthy females showed a random XCI pattern. Moreover, when comparing muscle with heart manifesting carriers, the former group showed a higher degree of skewing. No concordance in XCI was found between mothers and daughters, when symptomatic/asymptomatic mother-daughter pairs were analyzed. The results confirm that DMD clinical manifestations in carriers are associated with non-random patterns of X inactivation.


Subject(s)
Heterozygote , Muscular Dystrophy, Duchenne/genetics , X Chromosome Inactivation , Adolescent , Adult , Aged , Child , Dystrophin/genetics , Female , Humans , Middle Aged , Muscle, Skeletal/metabolism , Muscular Dystrophy, Duchenne/diagnosis , Myocardium/metabolism , Phenotype , Young Adult
4.
Gen Comp Endocrinol ; 178(1): 54-63, 2012 Aug 01.
Article in English | MEDLINE | ID: mdl-22531466

ABSTRACT

Endocrine disruptor chemicals (EDCs), which are predominantly present in the environment, are able to mimic or antagonise the biological activity of hormones primarily through the interaction with specific receptors. The main consequences are adverse effects on the growth and development of reproductive organs, the induction of cancer and effects on neuronal differentiation. In this study, we investigated the ability of certain EDCs, Bisphenol A (BPA), Bisphenol B (BPB), Bisphenol F (BPF), 4-n Nonylphenol (NP) and Octylphenol (OP), belonging to a homogeneous group of phenol origin, to interfere with specific cellular processes, namely, proliferation, by using MCF-7 breast carcinoma cells, and differentiation, by using murine bone marrow dendritic cells. We correlated the data on cell growth with the stimulation of cell cycle progression, which could become a step in the development of cancer, and we established a proliferation ranking between the tested EDCs: NP>BPA>OP>BPB>BPF. In addition, we investigated the ability of NP, BPA and OP to induce the differentiation of dendritic cells, the powerful antigen-presenting cells of the immune system. The differentiation and activation of these cells could affect a well-regulated immune response and determine an allergic sensitisation. We found that BPA and NP were active in determining differentiation.


Subject(s)
Cell Cycle/drug effects , Cell Differentiation/drug effects , Dendritic Cells/cytology , Dendritic Cells/drug effects , Endocrine Disruptors/pharmacology , Animals , Benzhydryl Compounds , Cell Line, Tumor , Cells, Cultured , Female , Humans , Mice , Mice, Inbred C57BL , Phenols/pharmacology
5.
Environ Toxicol Pharmacol ; 33(1): 9-15, 2012 Jan.
Article in English | MEDLINE | ID: mdl-22047638

ABSTRACT

Pregnant adult Balb-C mice were exposed daily to two different doses of Bisphenol A (BPA) by subcutaneous injection beginning on gestational day 1 through the seventh day after delivery. The mothers were sacrificed on postpartum day 21, and the offspring were sacrificed at 3 months of age. Control mice were subjected to the same experimental protocol but received saline injections. The liver, muscles, hindbrain and forebrain of the offspring were dissected and processed using HPLC to assess the level of BPA in the tissues and to determine its dependence on the exposure dose and gender. For comparison, the same tissues were dissected from the mothers and analysed. We report the following results: (1) the level of BPA that accumulated in a given tissue was dependent on the exposure dose; (2) the rank order of BPA accumulation in the various tissues was dependent on the gender of the offspring; (3) the average BPA concentrations in the liver and muscle of the female offspring were higher than in the males; and (4) the average BPA concentration in the central nervous system (i.e., the hindbrain and forebrain) of the male offspring was higher than in the females.


Subject(s)
Estrogens, Non-Steroidal/metabolism , Phenols/metabolism , Animals , Benzhydryl Compounds , Estrogens, Non-Steroidal/administration & dosage , Female , Humans , Male , Mice , Mice, Inbred BALB C , Phenols/administration & dosage , Pregnancy , Tissue Distribution
6.
Environ Toxicol Pharmacol ; 31(1): 198-204, 2011 Jan.
Article in English | MEDLINE | ID: mdl-21787686

ABSTRACT

Octylphenol (OP) is an endocrine-disrupting chemical that accumulates in various organs. It has also been shown to exert noxious effects on the central nervous system. In the present study, we measured in Sprague-Dawley rats the degree of OP accumulation in different areas of the brain and investigated the effect of OP in pain modulation. Two groups of male Sprague-Dawley rats were treated for 20 days with 50mg/kg BW/day of OP (group 1) or vehicle (group 2). At the end of the treatment, the formalin test was performed to evaluate the effect of OP exposure on pain. Soon after, rats were sacrificed, and the accumulation of OP in the cerebral cortex, hippocampus, hypothalamus, cerebellum, thalamus, striatum, mesencephalus and ventral hindbrain was measured by HPLC analysis. The results showed a greater accumulation of OP in the cerebral cortex compared to all the other areas; there was also more accumulation in the cerebellum compared to the mesencephalus and thalamus. No accumulation was found in the striatum. These results suggest that there is a preferential accumulation of OP in different areas of the brain with consequences to neural behaviour. On the contrary, experiments on facial grooming did not show significant effects of OP on pain.


Subject(s)
Brain/metabolism , Endocrine Disruptors/pharmacokinetics , Environmental Pollutants/pharmacokinetics , Environmental Pollution/adverse effects , Phenols/pharmacokinetics , Animals , Chromatography, High Pressure Liquid , Grooming/drug effects , Growth/drug effects , Male , Rats , Rats, Sprague-Dawley
7.
Chemosphere ; 82(3): 405-10, 2011 Jan.
Article in English | MEDLINE | ID: mdl-20971495

ABSTRACT

Bisphenol A (BPA) is an endocrine disruptor (ED) that is abundant in the environment because of its extensive use in human-manufactured products. In this study, the BPA concentration was measured in the muscle and liver of five edible fish, characterized by different habitat and habits, caught in two different sites of the Tyrrhenian Sea (Italy). Our results show that: (i) fish livers are about 2.5 times more polluted than muscle; (ii) fish caught in the Gulf of Naples are more polluted than those from the Latium coasts, ranging from 1.2-fold more for White Bream to 6.6-fold for Grey Mullet; and (iii) the percentages of fish found to be BPA-polluted in the Gulf of Naples ranged from 73% (for Bass) to 90% (for Mullet), while the Latium fish range from 60% (for Bass) to 90% (for Mullet). These data indicate that consumers of fish caught in the Gulf of Naples are at a greater risk for BPA-induced endocrine pathologies compared to those who consume fish caught along the Latium coasts.


Subject(s)
Endocrine Disruptors/metabolism , Fishes/metabolism , Phenols/metabolism , Water Pollutants, Chemical/metabolism , Animals , Benzhydryl Compounds , Environmental Monitoring , Italy , Liver/metabolism , Muscles/metabolism , Oceans and Seas , Seawater/chemistry
8.
Neuroscience ; 166(2): 416-21, 2010 Mar 17.
Article in English | MEDLINE | ID: mdl-20045451

ABSTRACT

The aim of the present study was to evaluate the production of superoxide anion (O(2)(-)) in the trigeminal complex nuclei after a functional mechanical overload of the teeth due to the preference for masticating on one side in rats. The preference for masticating on one side was induced by the discomfort due to a small abrasion of one molar; such lateralisation in mastication was confirmed by electromyography. The production of O(2)(-) was evaluated in the trigeminal nuclei by fluorescence microscopy after an injection of dihydroethidium. The results showed that there was an increased production of O(2)(-) in the subnucleus oralis of the spinal trigeminal nucleus in the same side where the mastication was preferred. This result demonstrates that an increased activity of non-painful sensory neurons can enhance the production of reactive oxygen species within the central second order sensory nuclei.


Subject(s)
Mastication/physiology , Superoxides/metabolism , Trigeminal Nuclei/metabolism , Afferent Pathways/metabolism , Analysis of Variance , Animals , Cold Temperature , Electromyography , Male , Microscopy, Fluorescence , Physical Stimulation , Rats , Rats, Sprague-Dawley , Sensory Receptor Cells/metabolism , Signal Processing, Computer-Assisted , Tooth
9.
J Muscle Res Cell Motil ; 30(3-4): 139-44, 2009.
Article in English | MEDLINE | ID: mdl-19526318

ABSTRACT

MyoD is a myogenic regulatory factor with a critical role in skeletal muscle development and regeneration. As muscle regeneration comes with an inflammatory process, it has been proposed that the inflammatory cells can play an important role in the induction of muscle fibres regeneration. The aim of the present work was to verify if a cyclooxygenase inhibitory drug (ketoprofen) would alter the normal expression of MyoD in a regenerating rat soleus muscle after an over-load lesion. Using immunohistochemical techniques, the numbers of m-cadherin-positive cells, a selective marker of satellite cells, and MyoD-positive cells were evaluated in functionally overloaded rat soleus muscles 4 days after a gastrocnemius tendon cut. The same study was conducted either with four rats injected with ketoprofen (100 mg/kg b.w./day) or with four rats injected with saline solution. The data obtained showed a very large decrease in the number of MyoD positive/m-cadherin positive cells in the ketoprofen injected group compared to the control group. Although further studies are needed to elucidate the sequence of biochemical events that induce a reduction of MyoD expression due to ketoprofen, the results demonstrate that prostaglandin synthesis is required for the induction of MyoD expression and that ketoprofen can affect this expression, with possible adverse effects on muscle regeneration.


Subject(s)
Cyclooxygenase Inhibitors/pharmacology , Ketoprofen/pharmacology , Muscle, Skeletal/drug effects , MyoD Protein/biosynthesis , Prostaglandins/biosynthesis , Regeneration/drug effects , Animals , Cadherins/metabolism , Male , Muscle, Skeletal/physiology , Rats , Rats, Sprague-Dawley
10.
Neuroscience ; 153(1): 182-8, 2008 Apr 22.
Article in English | MEDLINE | ID: mdl-18358626

ABSTRACT

The mechanisms of tolerance to subsequent episodes of ischemia induced by cortical spreading depression (CSD) are not clear. The effects of CSD on the expression of inducible nitric oxide synthase (iNOS), hypoxia inducible factor-1alpha (HIF-1alpha), and lactate dehydrogenase-A (LDH-A) were evaluated in the present experiment. Unilateral CSD was induced in Sprague-Dawley rats by application of KCl on the right cortex and the mRNA levels of iNOS, HIF-1alpha, and LDH-A were evaluated at 15 min, 2 h, 4 h, 6 h or 24 h after CSD. RT-PCR analysis showed: 1) an increase of iNOS mRNA at 15 min, 2 h, 4 h; 2) an increase of HIF-1alpha mRNA at 6 h; 3) an increase of LDH-A mRNA at 4 h. In situ hybridization with specific digoxigenin-labeled oligonucleotides revealed that the mRNA levels were increased at 15 min-2 h for iNOS, 2-4 h for LDH-A and 6 h for HIF-1 after CSD. Immunohistochemistry analysis revealed that levels of iNOS and HIF-1alpha were increased, respectively, at 2 h and 6 h after CSD. These data suggest that CSD promotes the expression of iNOS, HIF-1alpha, and LDH-A in nervous cells giving a neuroprotective effect.


Subject(s)
Cerebral Cortex/metabolism , Cortical Spreading Depression/physiology , Cytoprotection/genetics , Hypoxia-Inducible Factor 1, alpha Subunit/genetics , L-Lactate Dehydrogenase/genetics , Nitric Oxide Synthase Type II/genetics , Animals , Cell Survival/genetics , Cerebral Cortex/physiopathology , Gene Expression Regulation/genetics , Hypoxia-Inducible Factor 1, alpha Subunit/metabolism , Immunohistochemistry , Isoenzymes/genetics , Isoenzymes/metabolism , L-Lactate Dehydrogenase/metabolism , Lactate Dehydrogenase 5 , Male , Neurons/metabolism , Nitric Oxide/biosynthesis , Nitric Oxide Synthase Type II/metabolism , RNA, Messenger/metabolism , Rats , Rats, Sprague-Dawley , Time Factors , Up-Regulation/genetics
11.
Peptides ; 29(1): 120-6, 2008 Jan.
Article in English | MEDLINE | ID: mdl-18053616

ABSTRACT

Since experiments regarding a possible relation between olanzapine and orexin A has been scarcely reported in international literature, this experiment tested the effect of olanzapine on the sympathetic and thermogenic effects induced by orexin A. The firing rates of the sympathetic nerves to interscapular brown adipose tissue (IBAT), along with IBAT, colonic temperatures and heart rate were monitored in urethane-anesthetized male Sprague-Dawley rats before an injection of orexin A (1.5 nmol) into the lateral cerebral ventricle and over a period of 150 min after the injection. The same variables were monitored in rats with an intraperitoneal administration of olanzapine (10mg/kg bw), injected 30 min before the orexin administration. The results show that orexin A increases the sympathetic firing rate, IBAT, colonic temperatures and heart rate. This increase is blocked by the injection of olanzapine. These findings indicate that olanzapine affects the complex reactions related to activation of orexinergic system.


Subject(s)
Benzodiazepines/administration & dosage , Hyperthermia, Induced , Intracellular Signaling Peptides and Proteins/administration & dosage , Intracellular Signaling Peptides and Proteins/antagonists & inhibitors , Neuropeptides/administration & dosage , Neuropeptides/antagonists & inhibitors , Selective Serotonin Reuptake Inhibitors/administration & dosage , Sympathetic Nervous System/drug effects , Animals , Body Temperature/drug effects , Heart Rate/drug effects , Injections, Intraperitoneal , Injections, Intraventricular , Male , Olanzapine , Orexins , Rats , Rats, Sprague-Dawley
12.
Physiol Res ; 57(2): 269-273, 2008.
Article in English | MEDLINE | ID: mdl-17465698

ABSTRACT

Production of superoxide anions in the incubation medium of hippocampal slices can induce long-term potentiation (LTP). Other reactive oxygen species (ROS) such as hydrogen peroxide are able to modulate LTP and are likely to be involved in aging mechanisms. The present study explored whether intracerebro-ventricular (ICV) injection of oxidant or antioxidant molecules could affect LTP in vivo. With this aim in mind, field excitatory post-synaptic potentials (fEPSPs) elicited by stimulation of the perforant pathway were recorded in the dentate gyrus of the hippocampal formation in urethane-anesthetized rats. N-acetyl-L-cysteine, hydrogen peroxide (H2O2) or hypoxanthine/xanthine-oxidase solution (a superoxide producing system) were administrated by ICV injection. The control was represented by a group injected with saline ICV. Ten minutes after the injection, LTP was induced in the granule cells of the dentate gyrus by high frequency stimulation of the perforant pathway. Neither the H(2)O(2) injection or the N-acetyl-L-cysteine injection caused any variation in the fEPSP at the 10-min post-injection time point, whereas the superoxide generating system caused a significant increase in the fEPSP. Moreover, at 60 min after tetanic stimulation, all treatments attenuated LTP compared with the control group. These results show that ICV administration of oxidant or antioxidant molecules can modulate LTP in vivo in the dentate gyrus. Particularly, a superoxide producing system can induce potentiation of the synaptic response. Interestingly, ICV injection of oxidants or antioxidants prevented a full expression of LTP compared to the saline injection.


Subject(s)
Dentate Gyrus/physiology , Excitatory Postsynaptic Potentials/physiology , Free Radical Scavengers/metabolism , Long-Term Potentiation/physiology , Perforant Pathway/physiology , Acetylcysteine/administration & dosage , Acetylcysteine/metabolism , Analysis of Variance , Anesthetics, Intravenous/pharmacology , Animals , Dentate Gyrus/drug effects , Electric Stimulation , Excitatory Postsynaptic Potentials/drug effects , Free Radical Scavengers/administration & dosage , Hydrogen Peroxide/administration & dosage , Hydrogen Peroxide/metabolism , Hypoxanthine/administration & dosage , Injections, Intraventricular , Long-Term Potentiation/drug effects , Male , Oxidants/administration & dosage , Oxidants/metabolism , Oxidation-Reduction/drug effects , Rats , Rats, Sprague-Dawley , Urethane/pharmacology , Xanthine Oxidase/administration & dosage
13.
Regul Pept ; 139(1-3): 39-44, 2007 Mar 01.
Article in English | MEDLINE | ID: mdl-17134769

ABSTRACT

This experiment tested the effect of a lesion of cerebral catecholaminergic neurons on the sympathetic and thermogenic effects induced by an intracerebroventicular (icv) injection of orexin A. The firing rates of the sympathetic nerves to the interscapular brown adipose tissue (IBAT), along with IBAT, colonic temperatures and heart rate were monitored in urethane-anesthetized male Sprague-Dawley rats before an injection of orexin A (1.5 nmol) into the lateral cerebral ventricle and over a period of 150 min after the injection. Three days before the experiment, the rats were pre-treated with an icv injection of 6-hydroxydopamine (6-OHDA) or 6-OHDA plus desipramine or saline. The results show that orexin A increases the sympathetic firing rate, IBAT, colonic temperatures and heart rate in the rats pre-treated with saline. This increase is blocked by the pre-treatment with 6-OHDA alone or 6-OHDA plus desipramine. These findings indicate that cerebral catecholaminergic neurons (particularly the dopaminergic pathway) play a fundamental role in the complex reactions related to activation of the orexinergic system.


Subject(s)
Catecholamines/metabolism , Intracellular Signaling Peptides and Proteins/pharmacology , Neurons/drug effects , Neuropeptides/pharmacology , Sympathetic Nervous System/drug effects , Animals , Antipsychotic Agents/administration & dosage , Antipsychotic Agents/pharmacology , Body Temperature/drug effects , Cerebral Cortex/cytology , Cerebral Cortex/drug effects , Cerebral Cortex/metabolism , Desipramine/administration & dosage , Desipramine/pharmacology , Heart Rate/drug effects , Injections, Intraventricular , Intracellular Signaling Peptides and Proteins/administration & dosage , Male , Neurons/metabolism , Neurons/physiology , Neuropeptides/administration & dosage , Orexins , Oxidopamine/administration & dosage , Oxidopamine/pharmacology , Rats , Rats, Sprague-Dawley , Sympathetic Nervous System/cytology , Sympathetic Nervous System/physiology , Sympathomimetics/administration & dosage , Sympathomimetics/pharmacology , Thermogenesis/drug effects
14.
Neuropeptides ; 40(5): 357-63, 2006 Oct.
Article in English | MEDLINE | ID: mdl-17010428

ABSTRACT

Since no experiment regarding a possible relation between quetiapine and orexin A has been reported in international literature, this experiment tested the effect of quetiapine on the sympathetic and thermogenic effects induced by orexin A. The firing rates of the sympathetic nerves to interscapular brown adipose tissue (IBAT), along with IBAT, colonic temperatures and heart rate were monitored in urethane-anesthetized male Sprague-Dawley rats before an injection of orexin A (1.5 nmol) into the lateral cerebral ventricle and over a period of 150 min after the injection. The same variables were monitored in rats with an intraperitoneal administration of quetiapine (5 or 10 mg/kg bw), injected 30 min before the orexin administration. The results show that orexin A increases the sympathetic firing rate, IBAT, colonic temperatures and heart rate. This increase is delayed or reduced by the injection of quetiapine. These findings indicate that quetiapine affects the complex reactions related to activation of orexinergic system. Possible influences on the control of body weight and temperature are discussed.


Subject(s)
Antipsychotic Agents/pharmacology , Dibenzothiazepines/pharmacology , Fever/physiopathology , Intracellular Signaling Peptides and Proteins/pharmacology , Neuropeptides/pharmacology , Sympathetic Nervous System/drug effects , Adipose Tissue, Brown/drug effects , Adipose Tissue, Brown/physiology , Animals , Body Weight/drug effects , Electrodes, Implanted , Heart Rate/drug effects , Injections, Intraventricular , Intracellular Signaling Peptides and Proteins/administration & dosage , Male , Neuropeptides/administration & dosage , Orexins , Quetiapine Fumarate , Rats , Rats, Sprague-Dawley
15.
Physiol Res ; 55(1): 73-78, 2006.
Article in English | MEDLINE | ID: mdl-15857165

ABSTRACT

This experiment tested the effect of risperidone on the sympathetic and thermogenic effects induced by orexin A. The firing rates of sympathetic nerves to interscapular brown adipose tissue (IBAT), along with IBAT and colon temperatures and heart rate were monitored in urethane-anesthetized male Sprague-Dawley rats before an injection of orexin A (1.5 nmol) into the lateral cerebral ventricle and over a period of 2 hours after the injection. The same variables were monitored in rats with an intraperitoneal administration of risperidone (50 mg/kg bw), injected 30 min before the orexin administration. The results show that orexin A increases the sympathetic firing rate, IBAT, colonic temperatures and heart rate. This increase is enhanced by the injection of risperidone. These findings suggest that risperidone elevates the responses due to orexin, probably through an involvement of serotoninergic and dopaminergic pathways, which are affected by risperidone. Furthermore, we suggested the name "hyperthermine A" as additional denomination of "orexin A" by considering the strong influence of this neuropeptide on body temperature.


Subject(s)
Adrenergic Fibers/drug effects , Antipsychotic Agents/pharmacology , Hyperthermia, Induced , Intracellular Signaling Peptides and Proteins , Neuropeptides , Risperidone/pharmacology , Sympathomimetics , Action Potentials , Adipose Tissue, Brown/innervation , Animals , Antipsychotic Agents/administration & dosage , Body Temperature/drug effects , Drug Synergism , Heart Rate/drug effects , Injections, Intraperitoneal , Injections, Intraventricular , Male , Orexins , Rats , Rats, Sprague-Dawley , Risperidone/administration & dosage , Time Factors
16.
Eur J Neurosci ; 22(5): 1169-75, 2005 Sep.
Article in English | MEDLINE | ID: mdl-16176359

ABSTRACT

This experiment tested the involvement of the ventromedial hypothalamus (VMH) in the sympathetic and hyperthermic reactions induced by an intracerebroventricular (i.c.v.) injection of orexin A (1.5 nmol). In the first part of the experiment, the firing rate and cytochrome oxidase activity of the VMH neurons, and the colonic temperature were monitored in 12 urethane-anaesthetized rats before an i.c.v. injection of orexin and over a period of 2 h after the injection. Orexin induced an increase in the firing rate, colonic temperature and cytochrome oxidase activity. A group of 12 rats was used as a control: saline, but not orexin, was injected. No modifications in the firing rate, cytochrome oxidase reactivity and colonic temperature were noted. In the second part of the experiment, 12 rats were anaesthetized and lesioned bilaterally in the VMH with an injection of ibotenic acid. Sham lesions were carried out in 12 control rats. After 48 h, all animals were anaesthetized with ethyl-urethane. The firing rates of the sympathetic nerves to interscapular brown adipose tissue (IBAT), along with IBAT and colonic temperatures and heart rate were monitored before and over a period of 2 h after an i.c.v. injection of orexin or saline in the lesioned and sham-lesioned rats. Orexin increased the sympathetic firing rate, IBAT and colonic temperatures and heart rate in the sham-lesioned rats. These increases were reduced by lesion of VMH. Saline did not induce any modification. These findings indicate that the VMH is involved in the control of the orexin-induced hyperthermia.


Subject(s)
Hyperthermia, Induced , Intracellular Signaling Peptides and Proteins/pharmacology , Neuropeptides/pharmacology , Ventromedial Hypothalamic Nucleus/drug effects , Action Potentials/drug effects , Adipose Tissue, Brown/drug effects , Adipose Tissue, Brown/physiology , Animals , Electron Transport Complex IV/metabolism , Heart Rate/drug effects , Injections, Intraventricular/methods , Male , Neurons/drug effects , Orexins , Rats , Rats, Sprague-Dawley , Ventromedial Hypothalamic Nucleus/cytology , Ventromedial Hypothalamic Nucleus/physiology
17.
Transfusion ; 24(1): 22-4, 1984.
Article in English | MEDLINE | ID: mdl-6695436

ABSTRACT

The in vivo survival of Kn(a+)McC(a+) red cells in a patient with anti- "Kna/McCa" was studied using 51Cr-labeled incompatible cells. The antibody was IgG4, demonstrable at 37 degrees C in the antiglobulin test, and did not bind complement. Survival of tagged cells was 82.2 percent after 24 hours. The patient was transfused with a total of 29 units of incompatible blood with no evidence of ill effects. The direct antiglobulin test became positive after transfusion and remained positive for 2 1/2 months of observation. The findings suggest that individuals with anti- "Kna/McCa" may be transfused with Kn(a+)McC(a+) red cells.


Subject(s)
Blood Group Antigens/immunology , Blood Group Incompatibility/blood , Erythrocyte Aging , Isoantibodies/biosynthesis , Blood Group Incompatibility/diagnosis , Blood Group Incompatibility/immunology , Blood Transfusion , Coombs Test , Female , Humans , Middle Aged
18.
Transfusion ; 22(4): 329-32, 1982.
Article in English | MEDLINE | ID: mdl-7101426

ABSTRACT

A patient with pancytopenia and hypercellular bone marrow but without evidence of hemolysis had a positive direct antiglobulin test due to a red blood cell autoantibody. Although the patient was K-negative, eluates made from his erythrocytes contained what seemed to be anti-K antibody. The latter could be adsorbed onto and eluted from both K-positive and K-negative cells. We concluded that this patient is another example of autoanti-K antibody mimicking an alloanti-K antibody.


Subject(s)
Autoantibodies/analysis , Blood Group Antigens/immunology , Coombs Test , Isoantibodies/analysis , Kell Blood-Group System/immunology , Aged , Antibody Specificity , Autoantibodies/immunology , Blood Grouping and Crossmatching , Blood Transfusion , Erythrocytes/immunology , Humans , Isoantibodies/immunology , Male , Pancytopenia/immunology
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