Many-excitation removal of a transmon qubit using a single-junction quantum-circuit refrigerator and a two-tone microwave drive.

Achieving fast and precise initialization of qubits is a critical requirement for the successful operation of quantum computers. The combination of engineered environments with all-microwave techniques has recently emerged as a promising approach for the reset of superconducting quantum devices. In this work, we experimentally demonstrate the utilization of a single-junction quantum-circuit refrigerator (QCR) for an expeditious removal of several excitations from a transmon qubit. The QCR is indirectly coupled to the transmon through a resonator in the dispersive regime, constituting a carefully engineered environmental spectrum for the transmon. Using single-shot readout, we observe excitation stabilization times down to roughly 500 ns, a 20-fold speedup with QCR and a simultaneous two-tone drive addressing the e-f and f0-g1 transitions of the system. Our results are obtained at a 48-mK fridge temperature and without postselection, fully capturing the advantage of the protocol for the short-time dynamics and the drive-induced detrimental asymptotic behavior in the presence of relatively hot other baths of the transmon. We validate our results with a detailed Liouvillian model truncated up to the three-excitation subspace, from which we estimate the performance of the protocol in optimized scenarios, such as cold transmon baths and fine-tuned driving frequencies. These results pave the way for optimized reset of quantum-electric devices using engineered environments and for dissipation-engineered state preparation.

Stem cell mTOR signaling directs region-specific cell fate decisions during intestinal nutrient adaptation.

The adult intestine is a regionalized organ, whose size and cellular composition are adjusted in response to nutrient status. This involves dynamic regulation of intestinal stem cell (ISC) proliferation and differentiation. How nutrient signaling controls cell fate decisions to drive regional changes in cell-type composition remains unclear. Here, we show that intestinal nutrient adaptation involves region-specific control of cell size, cell number, and differentiation. We uncovered that activation of mTOR complex 1 (mTORC1) increases ISC size in a region-specific manner. mTORC1 activity promotes Delta expression to direct cell fate toward the absorptive enteroblast lineage while inhibiting secretory enteroendocrine cell differentiation. In aged flies, the ISC mTORC1 signaling is deregulated, being constitutively high and unresponsive to diet, which can be mitigated through lifelong intermittent fasting. In conclusion, mTORC1 signaling contributes to the ISC fate decision, enabling regional control of intestinal cell differentiation in response to nutrition.

An image analysis method for regionally defined cellular phenotyping of the Drosophila midgut.

The intestine is divided into functionally distinct regions along the anteroposterior (A/P) axis. How the regional identity influences the function of intestinal stem cells (ISCs) and their offspring remain largely unresolved. We introduce an imaging-based method, "Linear Analysis of Midgut" (LAM), which allows quantitative, regionally defined cellular phenotyping of the whole Drosophila midgut. LAM transforms image-derived cellular data from three-dimensional midguts into a linearized representation, binning it into segments along the A/P axis. Through automated multivariate determination of regional borders, LAM allows mapping and comparison of cellular features and frequencies with subregional resolution. Through the use of LAM, we quantify the distributions of ISCs, enteroblasts, and enteroendocrine cells in a steady-state midgut, and reveal unprecedented regional heterogeneity in the ISC response to a Drosophila model of colitis. Altogether, LAM is a powerful tool for organ-wide quantitative analysis of the regional heterogeneity of midgut cells.